1. Anti-Interleukin-10 Unleashes Transcriptional Response to Leishmanial Antigens in Visceral Leishmaniasis Patients.
- Author
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Singh, Om Prakash, Syn, Genevieve, Nylén, Susanne, Engwerda, Christian, Sacks, David, Wilson, Mary E, Kumar, Rajiv, Chakravarty, Jaya, Sundar, Shyam, Blackwell, Jenefer M, and Fakiola, Michaela
- Subjects
VISCERAL leishmaniasis ,TUMOR necrosis factors ,ANTIGENS ,CYTOKINE release syndrome ,LEISHMANIA donovani - Abstract
Visceral leishmaniasis (VL; Leishmania donovani) cases produce interferon-γ and tumor necrosis factor in response to soluble leishmanial antigen (SLA) in whole-blood assays. Using transcriptional profiling, we demonstrate the impact of interleukin-10 (IL-10), a cytokine implicated in VL, on this response. SLA stimulation identified 28 differentially expressed genes (DEGs), 17/28 in a single network with TNF as hub. SLA plus anti-IL-10 produced 454 DEGs, 292 in a single network with TNF, IFNG, NFKBIA, IL6, and IL1B as hubs in concert with a remarkable chemokine/cytokine storm. Our data demonstrate the singular effect of IL-10 as a potent immune modulator in VL. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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