1. Adoptive Transfer of Treg Cells Counters Adverse Effects of Toxoplasma gondii Infection on Pregnancy.
- Author
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Liu, Yang, Zhao, Mingdong, Xu, Xiaoyan, Liu, Xianbing, Zhang, Haixia, Jiang, Yuzhu, Zhang, Ling, and Hu, Xuemei
- Subjects
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TOXOPLASMA gondii , *PREGNANCY complications , *T cells , *LABORATORY mice , *APOPTOSIS - Abstract
Acute infection with Toxoplasma gondii (T. gondii) during pregnancy is associated with adverse outcomes. The mechanisms that cause this phenomenon are not clear. Regulatory T cells (Tregs) are involved in maternal tolerance, and here we observed a decrease in the absolute numbers of CTLA-4+ Tregs and PD-1+ Tregs in spleen and at the fetal-maternal interface in T. gondii-infected mice. Our results suggest that T. gondii induces apoptosis of Tregs. Additionally, we found that the expression of CTLA-4 and PD-1 on Tregs at fetal-maternal interface were higher than on spleen cells from normal pregnant mice. Therefore, we adoptively transferred Tregs from fetal-maternal interface or from spleens of normal pregnant mice into infected pregnant mice. Pregnancy outcomes were improved when Tregs were transferred from the fetal-maternal interface but not from the spleen. The mechanism appears to be through up-regulation of the number of CTLA-4+ Tregs and PD-1+ Tregs and correction of the imbalance between tolerant cytokines (IL-10, TGF-β) and inflammatory cytokines (IFN-γ). Our data indicate that Tregs at fetal-maternal interface express high levels of inhibitory molecules that play a vital immuno-protective role during pregnancy. [ABSTRACT FROM PUBLISHER]
- Published
- 2014
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