1. Gene expression profiling of fast- and slow-growing non-functioning gonadotroph pituitary adenomas.
- Author
-
Falch, Camilla Maria, Sundaram, Arvind Y. M., Øystese, Kristin Astrid, Normann, Kjersti Ringvoll, Lekva, Tove, Silamikelis, Ivars, Eieland, Alexander Kirkeby, Andersen, Marianne, Bollerslev, Jens, and Olarescu, Nicoleta Cristina
- Subjects
- *
GENE expression , *GONADOTROPIN , *ADENOMA - Abstract
Objective: Reliable biomarkers associated with aggressiveness of non-functioning gonadotroph adenomas (GAs) are lacking. As the growth of tumor remnants is highly variable, molecular markers for growth potential prediction are necessary. We hypothesized that fast- and slow-growing GAs present different gene expression profiles and reliable biomarkers for tumor growth potential could be identified, focusing on the specific role of epithelial-mesenchymal transition (EMT). Design and methods: Eight GAs selected for RNA sequencing were equally divided into fast- and slow-growing group by the tumor volume doubling time (TVDT) median (27.75 months). Data were analyzed by tophat2, cufflinks and cummeRbund pipeline. 40 genes were selected for RT-qPCR validation in 20 GAs based on significance, fold-change and pathway analyses. The effect of silencing MTDH (metadherin) and EMCN (endomucin) on in vitro migration of human adenoma cells was evaluated. Results: 350 genes were significantly differentially expressed (282 genes upregulated and 68 downregulated in the fast group, P-adjusted <0.05). Among 40 selected genes, 11 showed associations with TVDT (--0.669
- Published
- 2018
- Full Text
- View/download PDF