8 results
Search Results
2. Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs
- Author
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Phillip R. Bennett, Suraj Pavagada, Vasso Terzidou, David A. MacIntyre, Robert B. Channon, Sung Hye Kim, Jason Y. Chang, and Sylvain Ladame
- Subjects
Paper ,Chemistry, Multidisciplinary ,BIOMARKERS ,Oligonucleotides ,Catalysis ,Fluorescence ,chemistry.chemical_compound ,Materials Chemistry ,Humans ,NUCLEIC-ACIDS ,Circulating MicroRNA ,Oligonucleotide Array Sequence Analysis ,CELL-FREE MICRORNAS ,Science & Technology ,Oligonucleotide ,Chemistry ,Organic Chemistry ,Metals and Alloys ,RNA ,General Chemistry ,DNA ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Biochemistry ,Physical Sciences ,Ceramics and Composites ,Nucleic acid ,Biomarker (medicine) ,03 Chemical Sciences ,Quantitative analysis (chemistry) ,PNA - Abstract
Herein we demonstrate the first example of oligonucleotide-templated reaction (OTR) performed on paper, using lateral flow to capture and concentrate specific nucleic acid biomarkers on a test line. Quantitative analysis, using a low-cost benchtop fluorescence reader showed very high specificity down to the single nucleotide level and proved sensitive enough for amplification-free, on-chip, detection of endogenous concentrations of miR-150-5p, a recently identified predictive blood biomarker for preterm birth.
- Published
- 2019
3. 'All-in-Gel' design for supercapacitors towards solid-state energy devices with thermal and mechanical compliance
- Author
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Xinhua Liu, Chengyao Yin, Mengzheng Ouyang, Billy Wu, Rui Tan, Junjie Wei, Huizhi Wang, Samuel J. Cooper, Qigang Wang, Jie Zhou, Chandramohan George, Engineering & Physical Science Research Council (EPSRC), Engineering & Physical Science Research Council (E, and Innovate UK
- Subjects
GRAPHENE ,Technology ,Materials science ,Energy & Fuels ,Composite number ,Materials Science ,Materials Science, Multidisciplinary ,02 engineering and technology ,Electrolyte ,0915 Interdisciplinary Engineering ,Capacitance ,chemistry.chemical_compound ,ELECTRONICS ,Ionic conductivity ,General Materials Science ,0912 Materials Engineering ,Supercapacitor ,Science & Technology ,Renewable Energy, Sustainability and the Environment ,Chemistry, Physical ,General Chemistry ,0303 Macromolecular and Materials Chemistry ,021001 nanoscience & nanotechnology ,Chemistry ,chemistry ,Chemical engineering ,Ionic liquid ,Electrode ,Physical Sciences ,PAPER ,0210 nano-technology ,Current density ,ELECTROLYTE - Abstract
Ionogels are semi-solid, ion conductive and mechanically compliant materials that hold promise for flexible, shape-conformable and all-solid-state energy storage devices. However, identifying facile routes for manufacturing ionogels into devices with highly resilient electrode/electrolyte interfaces remains a challenge. Here we present a novel all-in-gel supercapacitor consisting of an ionogel composite electrolyte and bucky gel electrodes processed using a one-step method. Compared with the mechanical properties and ionic conductivities of pure ionogels, our composite ionogels offer enhanced self-recovery (retaining 78% of mechanical robustness after 300 cycles at 60% strain) and a high ionic conductivity of 8.7 mS cm−1, which is attributed to the robust amorphous polymer phase that enables facile permeation of ionic liquids, facilitating effective diffusion of charge carriers. We show that development of a supercapacitor with these gel electrodes and electrolytes significantly improves the interfacial contact between electrodes and electrolyte, yielding an area specific capacitance of 43 mF cm−2 at a current density of 1.0 mA cm−2. Additionally, through this all-in-gel design a supercapacitor can achieve a capacitance between 22–81 mF cm−2 over a wide operating temperature range of −40 °C to 100 °C at a current density of 0.2 mA cm−2.
- Published
- 2019
4. Paper-based microfluidic system for tear electrolyte analysis
- Author
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Yetisen, Ali K., Jiang, Nan, Tamayol, Ali, Ruiz-Esparza, Guillermo U., Zhang, Yu Shrike, Medina-Pando, Sofía, Gupta, Aditi, Wolffsohn, James S., Butt, Haider, Khademhosseini, Ali, and Yun, Seok-Hyun
- Subjects
Paper ,Technology ,Biochemistry & Molecular Biology ,Chemistry, Multidisciplinary ,OSMOLARITY ,DEVICE ,Biochemical Research Methods ,09 Engineering ,Analytical Chemistry ,Electrolytes ,Humans ,Nanoscience & Nanotechnology ,DRY-EYE DISEASE ,Instruments & Instrumentation ,Science & Technology ,DIAGNOSTIC-TESTS ,Chemistry, Analytical ,MATRIX-METALLOPROTEINASE-9 ,Sodium ,OCULAR SURFACE ,Equipment Design ,Microfluidic Analytical Techniques ,eye diseases ,DYSFUNCTION ,CONTACT-LENS ,Chemistry ,Tears ,Physical Sciences ,Potassium ,Science & Technology - Other Topics ,Dry Eye Syndromes ,HEALTH ,03 Chemical Sciences ,Life Sciences & Biomedicine ,FILM - Abstract
The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry eye subclassifications. Here, we demonstrate a portable microfluidic system that allows quantitative analysis of electrolytes in the tear fluid that is suited for point-of-care settings. The microfluidic system consists of a capillary tube for sample collection, a reservoir for sample dilution, and a paper-based microfluidic device for electrolyte analysis. The sensing regions are functionalized with fluorescent crown ethers, o-acetanisidide, and seminaphtorhodafluor that are sensitive to mono- and divalent electrolytes, and their fluorescence outputs are measured with a smartphone readout device. The measured sensitivity values of Na(+), K(+), Ca(2+) ions and pH in artificial tear fluid were matched with the known ion concentrations within the physiological range. The microfluidic system was tested with samples having different ionic concentrations, demonstrating the feasibility for the detection of early-stage dry eye, differential diagnosis of dry eye sub-types, and their severity staging.
- Published
- 2017
5. A paper-based in vitro model for on-chip investigation of the human respiratory system
- Author
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Rahim Rahimi, Babak Ziaie, Michael A.J. Zieger, Amir M. Ghaemmaghami, Rajiv Sood, Amy Donaldson, Su Su Htwe, Ali Tamayol, Manuel Ochoa, and Ali Khademhosseini
- Subjects
Paper ,0301 basic medicine ,Cell Culture Techniques ,Biomedical Engineering ,Bioengineering ,Nanotechnology ,Respiratory Mucosa ,02 engineering and technology ,Biochemistry ,Permeability ,03 medical and health sciences ,Oxygen permeability ,Lab-On-A-Chip Devices ,Ultimate tensile strength ,Monolayer ,Humans ,Mechanical Phenomena ,Aqueous solution ,Chemistry ,General Chemistry ,021001 nanoscience & nanotechnology ,In vitro ,Oxygen ,030104 developmental biology ,Membrane ,Tissue Array Analysis ,Cell culture ,Permeability (electromagnetism) ,Wettability ,Biophysics ,0210 nano-technology - Abstract
Culturing cells at the air–liquid interface (ALI) is essential for creating functional in vitro models of lung tissues. We present the use of direct-patterned laser-treated hydrophobic paper as an effective semi-permeable membrane, ideal for ALI cell culture. The surface properties of the paper are modified through a selective CO2 laser-assisted treatment to create a unique porous substrate with hydrophilic regions that regulate fluid diffusion and cell attachment. To select the appropriate model, four promising hydrophobic films were compared with each other in terms of gas permeability and long-term strength in an aqueous environment (wet-strength). Among the investigated substrates, parchment paper showed the fastest rate of oxygen permeability (3 times more than conventional transwell cell culture membranes), with the least variation in its dry and wet tensile strengths (124 MPa and 58 MPa, remaining unchanged after 7 days of submersion in PBS).The final paper-based platform provides an ideal, robust, and inexpensive device for generating monolayers of lung epithelial cells on-chip in a high-throughput fashion for disease modelling and in vitro drug testing.
- Published
- 2016
6. Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria
- Author
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Hadley D. Sikes, Shefali Lathwal, Massachusetts Institute of Technology. Department of Chemical Engineering, Lathwal, Shefali, and Sikes, Hadley
- Subjects
Paper ,Time Factors ,Computer science ,Biomedical Engineering ,Protozoan Proteins ,Color ,Bioengineering ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,Biochemistry ,Silver deposition ,Limit of Detection ,False positive paradox ,medicine ,Humans ,Malaria, Falciparum ,Colorimetry ,Detection limit ,Malarial infection ,Immunoassay ,Chromatography ,medicine.diagnostic_test ,010401 analytical chemistry ,General Chemistry ,Paper based ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Diagnosis of malaria ,0210 nano-technology - Abstract
Colorimetric detection methods that produce results readable by eye are important for diagnostic tests in resource-limited settings. In this work, we have compared three main types of colorimetric methods – enzymatic reactions, silver deposition catalyzed by gold nanoparticles, and polymerization-based amplification – in a paper-based immunoassay for detection of Plasmodium falciparum histidine-rich protein 2, a biomarker of malarial infection. We kept the binding events in the immunoassay constant in order to isolate the effect of the detection method on the outcome of the test. We have highlighted that the optimal readout time in a test can vary significantly – ranging from immediately after addition of a visualization agent to 25 minutes after addition of a visualization agent – depending on the colorimetric method being used, and accurate time keeping is essential to prevent false positives in methods where substantial color develops over time in negative tests. We have also shown that the choice of a colorimetric method impacts the calculated limit-of-detection, the ease of visual perception of the readout, and the total cost of the assay, and therefore directly impacts the feasibility and the ease-of-use of a test in field settings., Massachusetts Institute of Technology (Jonathan Whitney Fund), Massachusetts Institute of Technology (Haas Family Fellowship in Chemical Engineering), Massachusetts Institute of Technology (Charles E. Reed Faculty Initiative Fund)
- Published
- 2016
7. Nanopapers for organic solvent nanofiltration
- Author
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Koon-Yang Lee, Andreas Mautner, Alexander Bismarck, Kang Li, Minna Hakalahti, Panu Lahtinen, and Tekla Tammelin
- Subjects
Paper ,Flocculation ,Materials science ,Organic chemistry ,02 engineering and technology ,010402 general chemistry ,7. Clean energy ,01 natural sciences ,Catalysis ,law.invention ,Nanocellulose ,Fibre ,chemistry.chemical_compound ,law ,nanostructures ,Materials Chemistry ,SDG 7 - Affordable and Clean Energy ,Cellulose ,Organic Chemicals ,Filtration ,Organic solvent ,Papermaking ,Metals and Alloys ,Membranes, Artificial ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Nanostructures ,Membrane ,chemistry ,Chemical engineering ,membranes ,Ceramics and Composites ,Solvents ,Nanofiltration ,0210 nano-technology - Abstract
Would it not be nice to have an organic solvent nanofiltration membrane made from renewable resources that can be manufactured as simply as producing paper? Here the production of nanofiltration membranes made from nanocellulose by applying a papermaking process is demonstrated. Manufacture of the nanopapers was enabled by inducing flocculation of nanofibrils upon addition of trivalent ions.
- Published
- 2014
8. Smartphone-based simultaneous pH and nitrite colorimetric determination for paper microfluidic devices
- Author
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Dermot Diamond, Luis Fermín Capitán-Vallvey, Nuria López-Ruiz, Miguel M. Erenas, Fernando Benito-Lopez, Alberto J. Palma, and Vincenzo F. Curto
- Subjects
Paper ,Microfluidics ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,02 engineering and technology ,HSL and HSV ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Flash (photography) ,Light source ,Sampling (signal processing) ,Nitrite ,Nitrites ,Hue ,Chromatography ,010401 analytical chemistry ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Sample (graphics) ,0104 chemical sciences ,chemistry ,Colorimetry ,0210 nano-technology ,Biological system ,Cell Phone - Abstract
In this work, an Android application for measurement of nitrite concentration and pH determination in combination with a low-cost paper-based microfluidic device is presented. The application uses seven sensing areas, containing the corresponding immobilized reagents, to produce selective color changes when a sample solution is placed in the sampling area. Under controlled conditions of light, using the flash of the smartphone as a light source, the image captured with the built-in camera is processed using a customized algorithm for multidetection of the colored sensing areas. The developed image-processing allows reducing the influence of the light source and the positioning of the microfluidic device in the picture. Then, the H (hue) and S (saturation) coordinates of the HSV color space are extracted and related to pH and nitrite concentration, respectively. A complete characterization of the sensing elements has been carried out as well as a full description of the image analysis for detection. The results show good use of a mobile phone as an analytical instrument. For the pH, the resolution obtained is 0.04 units of pH, 0.09 of accuracy, and a mean squared error of 0.167. With regard to nitrite, 0.51% at 4.0 mg L(-1) of resolution and 0.52 mg L(-1) as the limit of detection was achieved.
- Published
- 2014
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