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To cope with the problem of global warming caused by greenhouse gases, an environmentally friendly working fluid with low global warming potential (GWP) value was proposed to replace R507A which has a higher GWP value. This paper evaluated the thermodynamic properties and cycle performance of the alternative named RTB1. Firstly, the thermodynamic properties of RTB1 were investigated experimentally, including saturated vapor pressure and miscibility with polyol ester (POE) lubricating oil. The results showed that the saturated vapor pressure of RTB1 is 7.14% higher than that of R507A on average, and RTB1 has good miscibility with POE 32. Secondly, the flammability of RTB1 was tested by the flammability limits test system, and the experimental results showed that RTB1 was a non-flammable working fluid. Finally, the cycle performance of RTB1 and R507A under different operating conditions was compared. RTB1 is better than R507A in terms of cooling capacity per unit volume and coefficient of performance, however, the discharge temperature of RTB1 is higher than that of R507A. This work is of great significance to promote the elimination process of high-GWP working fluids and reduce emissions of greenhouse gases. [ABSTRACT FROM AUTHOR]