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2. A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

Author

Ruiguo Wang, Xiaoou Su, Peilong Wang, and Wei Zhang

Subjects
Agonist, Paper, Multi residues, medicine.drug_class, Swine, Food Contamination, 02 engineering and technology, Fluorescent nanoparticles, Monoclonal antibody, 01 natural sciences, Fluorophotometry, Analytical Chemistry, Pork tissue, chemistry.chemical_compound, Cimaterol, Limit of Detection, medicine, Animals, Detection limit, Immunoassay, Original Paper, Chromatography, medicine.diagnostic_test, Chemistry, Mabuterol, Paper-based assay, 010401 analytical chemistry, Antibodies, Monoclonal, Adrenergic beta-Agonists, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Red Meat, β-Agonist, Clenbuterol, Nanoparticles, 0210 nano-technology, medicine.drug
Abstract

An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several β-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free β-agonists compete for the binding sites. This enables rapid screening for the 7 β-agonists in a single run that takes about 8 min. Detection limits for the seven β-agonists are

Published
2018