1. The Effect of a Lipid Surface Coating on the Permeation of Upconverting Nanoparticles through a 3D Human Lung Epithelial Model.
- Author
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Kim, Doyoung, Mandl, Gabrielle A., Balkota, Marta, Vernaz, Jimmy, Huang, Song, Constant, Samuel, Maechler, Pierre, DeWolf, Christine, Capobianco, John A., and Bonacina, Luigi
- Subjects
SKIN permeability ,MUCOCILIARY system ,LUNGS ,NANOPARTICLES ,CELL culture ,SURFACE properties - Abstract
Preclinical studies of nanoparticles for pulmonary therapeutics are often performed on 2D cell cultures or in vitro models that do not include a mucus barrier. However, the mucus layer lining the lungs is an essential barrier for drugs to permeate in order to exert a therapeutic effect. Herein, the role of surface coating of lanthanide‐doped upconverting nanoparticles (UCNPs) and their interaction with the mucus barrier are explored using a patient‐derived 3D cell culture model. The upconverted emissions from the UCNPs are used to track them throughout the 3D model and study their localization as a function of administration time and mucus thickness. Positively charged, ligand‐free, and negatively charged, supported lipid bilayer‐coated UCNPs are evaluated. A substantial difference in the residence time in mucus and mucociliary clearance of each type of UCNP is observed in a realistic and relevant model. As such, these results underscore the need for preclinical investigations in tissue models, especially with respect to the surface properties of the nanoparticles under study. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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