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Blood cell classification is widely used in the biomedical research. Appropriate separation between touching and overlapping blood cells is of great importance for the successful classification. In this paper, a novel algorithm based on shape information is proposed for the separation of partially overlapping blood cells. It is motivated by the problem of finding the borders between adjacent blood cells in microscopy images. The algorithm can not only separate the simple touching blood cells, but also large clusters of overlapping blood cells. In addition, this algorithm can be adopted for other applications, where separation between touching and overlapping particles is required. [ABSTRACT FROM AUTHOR]