4 results on '"Wang Rong"'
Search Results
2. MiR‐205‐5p promotes lung cancer progression and is valuable for the diagnosis of lung cancer.
- Author
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Zhao, Yu‐Long, Zhang, Jia‐Xiang, Yang, Juan‐Juan, Wei, Yu‐Bo, Peng, Jie‐Fei, Fu, Chang‐Jin, Huang, Min‐Hua, Wang, Rong, Wang, Ping‐Yu, Sun, Guang‐Bin, and Xie, Shu‐Yang
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DISEASE progression , *REVERSE transcriptase polymerase chain reaction , *FLOW cytometry , *CELL culture , *CANCER invasiveness , *LUNG tumors , *MICRORNA , *METASTASIS , *GENE expression , *IMMUNOBLOTTING , *CELL survival , *CELL proliferation , *GENES , *TUMOR markers , *POLYMERASE chain reaction , *RECEIVER operating characteristic curves - Abstract
Background: MicroRNAs (miRNAs) function as potential diagnostic biomarkers in various cancers. This study aimed to evaluate the roles of miR‐205‐5p in lung cancer progression and diagnosis. Materials and Methods: MiR‐205‐5p was detected by quantitative real‐time PCR. The effect of miR‐205‐5p on cell proliferation and metastasis was estimated by MTT and flow cytometry. The expression of TP53INP1 and related genes was analyzed by immunoblotting. The diagnostic value of miR‐205‐5p was analyzed using receiver operating characteristic (ROC) curve analysis, sensitivity, and specificity. Results: The miR‐205‐5p was increased in lung cancer tissues. MiR‐205‐5p mimics were promoted but its inhibitor suppressed cell proliferation and metastasis compared with control treatment in vitro and in vivo. By regulating the 3′ untranslated region, miR‐205‐5p could negatively regulate TP53INP1 expression, which further inhibited the expression of RB1 and P21, but increased that of cyclinD1. Moreover, the serum miR‐205‐5p levels of patients with lung cancer were significantly higher than those of normal controls, and they were correlated with patients' gender, drinking status, and clinical stage. The area under the ROC curve of serum miR‐205‐5p in the diagnosis of non‐small‐cell lung cancer was 0.8250, respectively. The finding supported its possession of high diagnostic efficiency for lung cancer. Conclusions: MiR‐205‐5p promoted lung cancer cell proliferation and metastasis by negatively regulating the novel target TP53INP1, which further affected the expression of P21, RB1, and cyclin D1. Serum miR‐205‐5p is a novel and valuable biomarker for lung cancer diagnosis. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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3. TIM-4 promotes the growth of non-small-cell lung cancer in a RGD motif-dependent manner.
- Author
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Zhang, Qianqian, Wang, Hongxing, Wu, Xiaodong, Liu, Bing, Liu, Wen, Wang, Rong, Liang, Xiaohong, Ma, Chunhong, and Gao, Lifen
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IMMUNOGLOBULINS , *T cells , *MUCINS , *NON-small-cell lung carcinoma , *CANCER invasiveness , *GENE expression , *IMMUNOHISTOCHEMISTRY , *GENETICS , *ANALYSIS of variance , *ANIMAL experimentation , *BINDING sites , *CELL lines , *CELL physiology , *CHI-squared test , *GENES , *LUNG cancer , *LUNG tumors , *MEMBRANE proteins , *MICE , *MYOCARDIAL infarction , *PROBABILITY theory , *STATISTICS , *TUMOR markers , *DATA analysis software , *LOG-rank test - Abstract
Background: T-cell immunoglobulin domain and mucin domain 4 (TIM-4) is exclusively expressed in antigen-presenting cells and involved in immune regulation. However, the role of TIM-4 expressed in tumour cells remains completely unknown.Methods: Immunohistochemistry staining was used to examine TIM-4 or Ki-67 expression in tumour tissues. Real-time PCR or RT-PCR was performed to detect TIM-4 mRNA expression. Lung cancer cell growth and proliferation were conducted by CCK-8 assay and EdU staining. Cell cycle progression was analysed by flow cytometry. The PCNA and cell cycle-related proteins were verified by western blot. Co-IP assay was used to identify the interaction of TIM-4 and integrin αvβ3. The efficacy of TIM-4 in vivo was evaluated using xenograft tumour model.Results: The expression of TIM-4 in non-small-cell lung cancer (NSCLC) tissues was significantly higher than that of the adjacent tissues. Enhanced TIM-4 expression was negatively correlated with histological differentiation of lung carcinoma and lifespan of patients. Overexpression of TIM-4 promoted lung cancer cell growth and proliferation, and upregulated the expression of PCNA, cyclin A, cyclin B1 and cyclin D1, accompanied by accumulation of lung cancer cells in S phase. Interestingly, Arg-Gly-Asp (RGD) motif mutation abolished the effect of TIM-4 on lung cancer cells, which was further verified by tumour xenografts in mice. Furthermore, we found that TIM-4 interacted with αvβ3 integrin through RGD motif.Conclusions: This finding suggests that TIM-4 might be a potential biomarker for NSCLC that promotes lung cancer progression by RGD motif. [ABSTRACT FROM AUTHOR]- Published
- 2015
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4. MiR-181b-5p Downregulates NOVA1 to Suppress Proliferation, Migration and Invasion and Promote Apoptosis in Astrocytoma.
- Author
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Zhi, Feng, Wang, Qiang, Deng, Danni, Shao, Naiyuan, Wang, Rong, Xue, Lian, Wang, Suinuan, Xia, Xiwei, and Yang, Yilin
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ASTROCYTOMAS , *APOPTOSIS , *MICRORNA , *GENE expression , *CANCER invasiveness - Abstract
MicroRNAs (miRNAs) are small, short noncoding RNAs that modulate the expression of numerous genes by targeting their mRNA. Numerous abnormal miRNA expression patterns are observed in various human malignancies, and certain miRNAs can act as oncogenes or tumor suppressors. Astrocytoma, the most common neuroepithelial cancer, represents the majority of malignant brain tumors in humans. In our previous studies, we found that the downregulation of miR-181b-5p in astrocytomas is associated with a poor prognosis. The aim of the present study was to investigate the functional role of miR-181b-5p and its possible target genes. miR-181b-5p was significantly downregulated in astrocytoma specimens, and the reduced expression of miR-181b-5p was inversely correlated with the clinical stage. The ectopic expression of miR-181b-5p inhibited proliferation, migration and invasion and induced apoptosis in astrocytoma cancer cells in vitro. The NOVA1 (neuro-oncological ventral antigen 1) gene was further identified as a novel direct target of miR-181b-5p. Specifically, miR-181b-5p bound directly to the 3'-untranslated region (UTR) of NOVA1 and suppressed its expression. In clinical specimens, NOVA1 was overexpressed, and its protein levels were inversely correlated with miR-181b-5p expression. Furthermore, the changing level of NOVA1 was significantly associated with a poor survival outcome. Similar to restoring miR-181b-5p expression, downregulating NOVA1 inhibited cell growth, migration and invasion. Overexpression of NOVA1 reversed the inhibitory effects of miR-181b-5p. Our results indicate that miR-181b-5p is a tumor suppressor in astrocytoma that inhibits tumor progression by targeting NOVA1. These findings suggest that miR-181b-5p may serve as a novel therapeutic target for astrocytoma. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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