1. Anti-Inflammatory Activities of Leaf Oil from Cinnamomum subavenium In Vitro and In Vivo.
- Author
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Hao, Xincai, Sun, Weiguang, Ke, Changbin, Wang, Fuqian, Xue, Yongbo, Luo, Zengwei, Wang, Xuanbin, Zhang, Jinwen, and Zhang, Yonghui
- Subjects
INFLAMMATION treatment ,ANIMAL experimentation ,ANTI-inflammatory agents ,CELL lines ,EDEMA ,INTERLEUKINS ,MEDICINAL plants ,MICE ,NITRIC oxide ,PROSTAGLANDINS E ,TUMOR necrosis factors ,VEGETABLE oils ,DNA-binding proteins ,NITRIC-oxide synthases ,MALONDIALDEHYDE ,PLANT extracts ,IN vitro studies ,IN vivo studies ,PHARMACODYNAMICS - Abstract
The study determined the chemical constituents and anti-inflammatory effects of leaf oil from Cinnamomum subavenium (CS-LO) that has been used in folk medicine to treat various symptoms including inflammation. The anti-inflammatory effects of the oil were evaluated by LPS-stimulated RAW264.7 cells and the Carr-induced hind mouse paw edema model, respectively. In vitro, nitric oxide (NO), prostaglandin E2 (PGE
2 ), TNF-α, IL-6, and IL-1β were significantly decreased by CS-LO, and the expression of nuclear factor-κB (NF-κB) protein was blocked as well. In in vivo, the malondialdehyde (MDA) and paw edema levels were decreased by CS-LO, and the same result came up on the NO and tumor necrosis factor (TNF-a) of serum at the 5th h after Carr injection. In addition, iNOS and COX-2 immunoreactive cells of the paw tissue were decreased significantly by CS-LO (200 mg/kg) in histological examination. The present findings indicated that CS-LO have anti-inflammatory properties, and the effects might be caused through inhibiting iNOS, COX-2, TNF-α, IL-1β, and IL-6 expression via affecting NF-κB pathway, which will provide a power scientific basis for CS-LO to be used as the treatment of inflammatory diseases. [ABSTRACT FROM AUTHOR]- Published
- 2019
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