1. CLC-3 Channels Modulate Excitatory Synaptic Transmission in Hippocampal Neurons
- Author
-
Wang, Xue Qing, Deriy, Ludmila V., Foss, Sarah, Huang, Ping, Lamb, Fred S., Kaetzel, Marcia A., Bindokas, Vytautas, Marks, Jeremy D., and Nelson, Deborah J.
- Subjects
- *
NEURAL transmission , *NERVOUS system , *CELL membranes , *IMMUNOHISTOCHEMISTRY - Abstract
Summary: It is well established that ligand-gated chloride flux across the plasma membrane modulates neuronal excitability. We find that a voltage-dependent Cl− conductance increases neuronal excitability in immature rodents as well, enhancing the time course of NMDA receptor-mediated miniature excitatory postsynaptic potentials (mEPSPs). This Cl− conductance is activated by CaMKII, is electrophysiologically identical to the CaMKII-activated CLC-3 conductance in nonneuronal cells, and is absent in clc-3−/− mice. Systematically decreasing [Cl−]i to mimic postnatal [Cl−]i regulation progressively decreases the amplitude and decay time constant of spontaneous mEPSPs. This Cl−-dependent change in synaptic strength is absent in clc-3 −/− mice. Using surface biotinylation, immunohistochemistry, electron microscopy, and coimmunoprecipitation studies, we find that CLC-3 channels are localized on the plasma membrane, at postsynaptic sites, and in association with NMDA receptors. This is the first demonstration that a voltage-dependent chloride conductance modulates neuronal excitability. By increasing postsynaptic potentials in a Cl− dependent fashion, CLC-3 channels regulate neuronal excitability postsynaptically in immature neurons. [Copyright &y& Elsevier]
- Published
- 2006
- Full Text
- View/download PDF