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27 results on '"Jacobs, Joe"'

1. A Chiral-LC-MS Method for the Simultaneous Quantification of Short-Chain Fatty Acids and D/L-Lactate in the Ruminal Fluid of Dairy Cows.

Author

Liu, Zhiqian, Williams, S. Richard O., Jacobs, Joe L., Neachtain, Aodan S. O., and Rochfort, Simone

Subjects
SHORT-chain fatty acids, DAIRY cattle, FATTY acids, FLUIDS, BLOOD lactate, LIQUID chromatography-mass spectrometry, LACTATES
Abstract

Short-chain fatty acids (SCFA) and lactate in ruminal fluid are products resulting from the microbial fermentation of substrates and can be used to reflect the composition and activity of the ruminal microbiome. Determination of SCFA and D-/L-lactate in ruminal fluid currently requires two separate protocols, which is time-consuming and costly. In this study, we have optimised and validated a simple and unified 3-nitrophenylhydrazine (3-NPH) derivatisation protocol and a 20 min chiral-LC-MS method for the simultaneous quantification of all SCFA and D- and L-lactate in ruminal fluid. This method, which requires no sample pretreatment or purification shows adequate sensitivity (limit of detection (LOD): 0.01 µg/mL), satisfactory accuracy (recovery: 88–103%), and excellent reproducibility (relative standard deviation (RSD) for repeated analyses < 3% for most analytes). The application of this method to a cohort of 24 animals allowed us to reveal a large inter-cow variation in ruminal SCFA and lactate level, the concentration range for each species, the widespread correlation between different SCFA, and the strong correlation between D- and L-lactate. [ABSTRACT FROM AUTHOR]

Published
2024
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10. What machines are used to identify DNA in a forensic laboratory?

Author

Jacobs, Joe

Subjects
DNA, Machinery, Magneto-electric machines, Science and technology
Abstract

A thermostatic cycler can separate and amplify the sample, creating copies. This is useful if the sample is small or has deteriorated. A DNA analyser is used to match samples [...]

Published
2021

21. Microbium

Author

Jacobs, Joela and Malinowska, Agnes

Subjects
animalcules, bacteria, microbiome, microbiology, viruses, fungi, lichen, protozoa, pollen, coral, bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PSG Microbiology (non-medical), bic Book Industry Communication::R Earth sciences, geography, environment, planning::RN The environment::RNA Environmentalist thought & ideology
Abstract

Microbium: The Neglected Lives of Micro-matter tells the story of small matter such as bacteria, coral, fungi, lichen, pollen, protozoa, and viruses. With short entries that are organized like a herbarium or similar specimen collection, the book is a “microbium”—both the term for a single microbe and a play on “microbiome.” As such, Microbium makes visible the often overseen but huge impact of miniscule matter on human culture and the environment. Each entry is a “microscopic reading” that describes the natural history and scientific discovery of a particular form of micro-matter, while also telling a story about the cultural and artistic roles it has played over the centuries. From the poetry of Emily Dickinson to the “coralness” of coral reefs to contemporary literature about the COVID-19 pandemic, this book places micro-matter under a cultural microscope and translates the significance of the invisible interspecies social realm to the human scale, magnifying the many ways in which micro-matter matters. Ultimately, Microbium shows the potential of micro-matter to teach us how to revitalize our political and cultural systems, habits of thought, and aesthetic or representational modes.

Published
2023
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22. mail call.

Author

Conway, James T., White, Jim, Topliff, Gregory, Benaci, Joe, Jacobs, Joe, Dillon, K. W., Hall, Wynton, Johnson, Dan E., Thompson, Steve D., Harmening, Don, Triggs, Ronald E., Ruyle, Thomas M., Krohn, Charles, and Hemingway, Al

Subjects
LETTERS to the editor, AMERICAN veterans
Abstract

Several letters to the editor are presented in response to articles published in previous issues, including "Valor in the face of the enemy," in the November 2006 issue, "I am a liar: Phony vets" in the November 2006 issue and about the social class of those who served.

Published
2007

23. Letters.

Author

Dinovitz, Noam, McClain, John, Billing, Karen, Dapkey, Rita M., Poirier, Michael P., Nigro, Samuel A., Herold, Rollin, Jacobs, Joe, Lynch, Frank, Calhoun, Zach, and Nichols, Elizabeth G.

Subjects
LETTERS to the editor, SPORTS teams, BASEBALL teams, FOOTBALL teams, DEFENSIVE backs (Football)
Abstract

Several letters to the editor are presented in response to articles from the October 27, 2008 issue, including "Historically, We Suck," "Go Strong or Get Toasted," and "Inside the Week in Sports."

Published
2008

24. Should the U.S. undertake nation-building/peacekeeping mission?

Author

Trombley Jr., James F., Wallace, Don, Du Bos, S., West, Robert E., Mac Kinnon, Robert, French, John D., Roper, Burns H., Du Bois, John A., Jacobs, Joe, Houston, Dean, Densmore, Chris, Clements, Royce, Freitag, Louis, Parshall, David, Valdez, Jess, Olberding, Francis A., and Wolf, Harlan Keith

Subjects
PEACE treaties, NATION building, POLITICAL development, MEDICAL care
Abstract

Presents views on the nation-building and peacekeeping missions of the U.S. government. Urge for the United Nations (UN) to play a larger role to help developing countries; Questions for the leaders to fix the UN so it will be able to do its mission; Focus of the mission on helping the U.S. military for medical care.

Published
2005

25. Letters.

Author

Kramer, Dick, Gutierrez, Michael, Gries, Ron, Zeman, Richard A., Jacobs, Joe, Randall, Jack, Covey, Keith A., Heller, Charles O., Reale, Anthony, and Williams, Doug

Subjects
LETTERS to the editor, SPORTS
Abstract

Presents several letters to the editor from readers in response to articles previously appearing in "Sports Illustrated." Letters discussing the year-end double issue, sport and science, sexual activity of athletes, sports in Oklahoma, racism in sports, and engaging in sporting activities with family members.

Published
2004

27. Lipid Oxidation and Colour Stability of Lamb and Yearling Meat (Muscle Longissimus Lumborum ) from SheepSupplemented with Camelina-Based Diets after Short-,Medium-, and Long-Term Storage.

Author

Ponnampalam EN, Butler KL, Muir SK, Plozza TE, Kerr MG, Brown WG, Jacobs JL, and Knight MI

Abstract

This study investigated the impact of feeding pelleted diets containing camelina ( Camelina sativa L. Crantz) hay (CAHP) or camelina meal (CAMP) as a supplement compared with a control pellet (CONP) diet, without vitamin E fortification. The fatty acid profile, retail colour, and lipid oxidative stability of lamb and yearling meat ( m. longissimus lumborum ) stored for short-, medium-, or long-periods (2 days (fresh), 45 days and 90 days) under chilled to semi-frozen conditions were determined. The CAMP diet altered key fatty acids ( p < 0.05) in a nutritionally beneficial manner for human health compared to the other diets, with increased total omega-3, decreased omega-6 fatty acids and decreased omega-6/omega-3 ratio of muscle. Muscle vitamin E concentration was lower ( p < 0.05) for both camelina diets (CAMP and CAHP) when compared with the CONP diet, with the average concentrations less than 1 mg/kg muscle for all three treatments. Animal type and storage length were factors that all affected ( p < 0.05) colour and lipid oxidative stability of meat. These results emphasise the importance of vitamin E concentration in meat stored for extended periods under semi-frozen conditions to maintain desirable meat colour during retail display, and to avoid off-flavour development of the cooked meat.

Published
2021
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