1. Poly-functional T helper cells in human tonsillar mononuclear cells.
- Author
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Yu S, Luo X, Yang B, Xiao L, Wu X, Li H, and Wu C
- Subjects
- Adolescent, Adult, B-Lymphocytes cytology, B-Lymphocytes immunology, CD8-Positive T-Lymphocytes cytology, CD8-Positive T-Lymphocytes immunology, Female, Flow Cytometry, Humans, Interferon-gamma genetics, Interferon-gamma immunology, Interleukin-17 genetics, Interleukin-17 immunology, Interleukin-2 genetics, Interleukin-2 immunology, Interleukins genetics, Interleukins immunology, Ionomycin pharmacology, Lymphocyte Activation, Male, Palatine Tonsil cytology, Primary Cell Culture, Single-Cell Analysis, T-Lymphocytes, Helper-Inducer cytology, T-Lymphocytes, Helper-Inducer drug effects, Tetradecanoylphorbol Acetate pharmacology, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha immunology, Interleukin-22, Gene Expression immunology, Palatine Tonsil immunology, T-Lymphocytes, Helper-Inducer immunology
- Abstract
Tonsils are important lymphoid organs in which B cells and T cells complete their maturation and identify cells that are infected by pathogens. However, the functions of T cells in human tonsils remain unclear, especially the characteristics of polyfunctional CD4
+ T helper cells. In this study, we used multi-color flow cytometry to analyze the expression or co-expression of effector cytokines in CD4+ T cells from tonsillar tissues. We have demonstrated that tonsillar CD4+ T cell can express various Th effector cytokines after short-term polyclonal stimulation, and that cytokine-producing CD4+ T cells were CD45RO+ T cells. In addition, we analyzed the co-expression of two or more kinds of cytokines at the level of a single cell. The results showed that tonsillar CD4+ T cells exhibited polyfunctionality by co-expressing two to five kinds of cytokines in the same time. These data furnished a basic theory for further understanding the differentiation of polyfunctional Th cells in human tonsils and their functions in resisting invasive microorganisms.- Published
- 2019
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