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Your search keyword '"Manfred T. Reetz"' showing total 2 results
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2 results on '"Manfred T. Reetz"'

1. A genetic selection system for evolving enantioselectivity of enzymes.

Author

Manfred T. Reetz, Horst Höbenreich, Pankaj Soni, and Layla Fernández

Subjects
*ENZYMES, *ORGANIC compounds, *ENANTIOSELECTIVE catalysis, *LIPASES, *HYDROLYSIS, *CHEMICAL kinetics
Abstract

As an alternative to screening in the directed evolution of enantioselective enzymes, a selection system has been implemented for a lipase-catalyzed hydrolytic kinetic resolution of a chiral ester. [ABSTRACT FROM AUTHOR]

Published
2008
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2. Directed evolution and axial chirality: optimization of the enantioselectivity of Pseudomonas aeruginosa lipase towards the kinetic resolution of a racemic allene.

Author

José Daniel Carballeira, Patrik Krumlinde, Marco Bocola, Andreas Vogel, Manfred T. Reetz, and Jan-E. Bäckvall

Subjects
CHIRALITY, PSEUDOMONAS aeruginosa, LIPASES, ALLENE
Abstract

Directed evolution of Pseudomonas aeruginosa lipase by the use of combinatorial active site saturation test (CAST) criteria provided a highly enantioselective mutant (Leu162Phe) for kinetic resolution of an axially chiral allene, p-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate (E = 111); the high enantioselectivity of the Leu162Phe mutant was rationalized by π–π stacking. [ABSTRACT FROM AUTHOR]

Published
2007
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