11 results on '"Mortaz, Esmaeil"'
Search Results
2. Is There an Ending in Insight for COVID-19.
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Mortaz, Esmaeil, Jamaati, Hamidreza, and Adcock, Ian M.
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SARS-CoV-2 , *COVID-19 , *SARS-CoV-2 Omicron variant , *SARS-CoV-2 Delta variant - Published
- 2021
3. Serum cytokine levels of COVID-19 patients after 7 days of treatment with Favipiravir or Kaletra.
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Mortaz, Esmaeil, Bassir, Ali, Dalil Roofchayee, Neda, Dezfuli, Neda K., Jamaati, Hamidreza, Tabarsi, Payam, Moniri, Afshin, Rezaei, Mitra, Mehrian, Payam, Varahram, Mohammad, Marjani, Majid, Mumby, Sharon, and Adcock, Ian M.
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COVID-19 , *CYTOKINES , *COMPUTED tomography , *LOPINAVIR-ritonavir , *TUMOR necrosis factors , *C-reactive protein , *CYTOKINE release syndrome - Abstract
• Anti-viral therapy significantly. • Reduced CT scan scores and the elevated serum levels of CRP and LDH, serum levels of IL-6, CXCL8 and IFNγ were elevated at baseline in COVID-19. • IL-1β and TNFα levels were also enhanced after treatment. • Antiviral treatments did not suppress the inflammatory phase of COVID-19 after 7 days treatment although CT, CRP and LDH suggest a decline in lung inflammation. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease 2019 (COVID-19) has infected 86,4 M patients and resulted in 1,86 M deaths worldwide. Severe COVID-19 patients have elevated blood levels of interleukin-6 (IL-6), IL-1β, tumor necrosis factor (TNF)α, IL-8 and interferon (IFN)γ. To investigate the effect of antiviral treatment serum cytokines in severe COVID-19 patients. Blood was obtained from 29 patients (aged 32–79 yr) with laboratory-confirmed COVID-19 upon admission and 7 days after antiviral (Favipiravir or Lopinavir/Ritonavir) treatment. Patients also received standard supportive treatment in this retrospective observational study. Chest computed tomography (CT) scans were evaluated to investigate lung manifestations of COVID-19. Serum was also obtained and cytokines levels were evaluated. 19 age- and gender-matched healthy controls were studied. Anti-viral therapy significantly reduced CT scan scores and the elevated serum levels of C-reactive protein (CRP) and lactate dehydrogenase (LDH). In contrast, serum levels of IL-6, IL-8 and IFNγ were elevated at baseline in COVID-19 subjects compared to healthy subjects with IL-6 (p = 0.006) and IL-8 (p = 0.011) levels being further elevated after antiviral therapy. IL-1β (p = 0.01) and TNFα (p = 0.069) levels were also enhanced after treatment but baseline levels were similar to those of healthy controls. These changes occurred irrespective of whether patients were admitted to the intensive care unit. Antiviral treatments did not suppress the inflammatory phase of COVID-19 after 7 days treatment although CT, CRP and LDH suggest a decline in lung inflammation. There was limited evidence for a viral-mediated cytokine storm in these COVID-19 subjects. [ABSTRACT FROM AUTHOR]
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- 2021
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4. Immune cell profiling and antibody responses in patients with COVID-19.
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Rezaei, Mitra, Mahmoudi, Shima, Mortaz, Esmaeil, and Marjani, Majid
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ANTIBODY formation , *COVID-19 , *LEUCOCYTES , *KILLER cells , *LYMPHOCYTE subsets - Abstract
Background: Although there are a growing number of studies on evaluating lymphocyte subset counts as prognostic factors for COVID-19 disease severity, the lymphocyte subsets' analyses of both IgM and IgG responders and non-responders during the periods after onset of symptoms, have not been conducted yet. So, this study aimed to evaluate immune cell profiling of COVID-19 patients with and without antibody responses.Methods: In this cross-sectional study, the levels of peripheral lymphocyte subsets were measured using flow cytometry in 53 patients with positive SARS-CoV-2 RT-PCR, for whom antibody testing of COVID-19 was performed.Results: The white blood cell, neutrophil, and lymphocyte counts consistently decreased in the IgM and IgG non-responder group, while the differences in the median value between the two study groups were found to be statistically significant only in terms of neutrophil counts (P = 0.024 for IgM response and p-value = 0.046 for IgG response, respectively). Moreover, the level of neutrophil-to-lymphocyte ratio was observed to be significantly lower in the IgM or IgG non-responder group compared to the IgM or IgG responder group (3.6 ± 3.1 vs. 6.3 ± 4.2; p-value = 0.021). The patients with IgM antibody response had a significantly lower CD20+ lymphocytes (11% versus 15% in the groups without IgM antibody response, p-value = 0.031), The percentages of NK cells and CD4+ T cells significantly increased in the patients with IgG antibody response compared to those without IgG antibody response (13% versus 10%, p-value = 0.028, and 41.5% versus 34%; p-value = 0.03, respectively). Moreover, the patients who produced IgM or IgG antibody had significantly higher percentages of total T lymphocytes (64% versus 54%; p-value = 0.017), CD4+ T cells (41% versus 34%; p-value = 0.038), and NK cells (13% versus 9%, p-value = 0.023) compared to the group with no serological response. No significant difference was observed in the percentage of other lymphocyte subsets, including CD8+ T cells, Treg cells, and CD19+ B cells.Conclusion: Our results suggest that the total T cells, CD4+ T cells, and NK cells percentages are linked to serological response. Moreover, our findings suggested that neutrophil absolute counts and neutrophil-to-lymphocyte ratio may be valuable predictors of IgM or IgG antibody response. [ABSTRACT FROM AUTHOR]- Published
- 2021
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5. Possible cancer-causing capacity of COVID-19: Is SARS-CoV-2 an oncogenic agent?
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Jahankhani, Kasra, Ahangari, Fatemeh, Adcock, Ian M., and Mortaz, Esmaeil
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SARS-CoV-2 , *COVID-19 , *CANCER stem cells - Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has shown diverse life-threatening effects, most of which are considered short-term. In addition to its short-term effects, which has claimed many millions of lives since 2019, the long-term complications of this virus are still under investigation. Similar to many oncogenic viruses, it has been hypothesized that SARS-CoV-2 employs various strategies to cause cancer in different organs. These include leveraging the renin angiotensin system, altering tumor suppressing pathways by means of its nonstructural proteins, and triggering inflammatory cascades by enhancing cytokine production in the form of a "cytokine storm" paving the way for the emergence of cancer stem cells in target organs. Since infection with SARS-CoV-2 occurs in several organs either directly or indirectly, it is expected that cancer stem cells may develop in multiple organs. Thus, we have reviewed the impact of coronavirus disease 2019 (COVID-19) on the vulnerability and susceptibility of specific organs to cancer development. It is important to note that the cancer-related effects of SARS-CoV-2 proposed in this article are based on the ability of the virus and its proteins to cause cancer but that the long-term consequences of this infection will only be illustrated in the long run. • The long-term complications of this SARS-Cov-2 is still under investigation. • SARS-CoV-2 can employ various strategies to potentially cause cancer in different organs. • SARS-CoV-2 infects several organs which may enable cancer stem cells to develop in multiple organs. • Cancer-related effects of SARS-CoV-2 may take several years to manifest themselves. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Immunophenotype and function of circulating myeloid derived suppressor cells in COVID-19 patients.
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Kiaee, Fatemeh, Jamaati, Hamidreza, Shahi, Heshmat, Roofchayee, Neda Dalil, Varahram, Mohammad, Folkerts, Gert, Garssen, Johan, Adcock, Ian M., and Mortaz, Esmaeil
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MYELOID-derived suppressor cells , *SARS-CoV-2 , *REGULATORY T cells , *SUPPRESSOR cells , *COVID-19 , *T cells - Abstract
The pathogenesis of coronavirus disease 2019 (COVID-19) is not fully elucidated. COVID-19 is due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which causes severe illness and death in some people by causing immune dysregulation and blood T cell depletion. Increased numbers of myeloid-derived suppressor cells (MDSCs) play a diverse role in the pathogenesis of many infections and cancers but their function in COVID-19 remains unclear. To evaluate the function of MDSCs in relation with the severity of COVID-19. 26 PCR-confirmed COVID-19 patients including 12 moderate and 14 severe patients along with 11 healthy age- and sex-matched controls were enrolled. 10 ml whole blood was harvested for cell isolation, immunophenotyping and stimulation. The immunophenotype of MDSCs by flow cytometry and T cells proliferation in the presence of MDSCs was evaluated. Serum TGF-β was assessed by ELISA. High percentages of M-MDSCs in males and of P-MDSCs in female patients were found in severe and moderate affected patients. Isolated MDSCs of COVID-19 patients suppressed the proliferation and intracellular levels of IFN-γ in T cells despite significant suppression of T regulatory cells but up-regulation of precursor regulatory T cells. Serum analysis shows increased levels of TGF-β in severe patients compared to moderate and control subjects (HC) (P = 0.003, P < 0.0001, respectively). The frequency of MDSCs in blood shows higher frequency among both moderate and severe patients and may be considered as a predictive factor for disease severity. MDSCs may suppress T cell proliferation by releasing TGF-β. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Effects of toll-like receptor agonists and SARS-CoV-2 antigens on interferon (IFN) expression by peripheral blood CD3+ T cells from COVID-19 patients.
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Abdolmohammadi-Vahid, Samaneh, Baradaran, Behzad, Sadeghi, Armin, Bezemer, Gillina F.G., Kiaee, Fatemeh, Adcock, Ian M., Folkerts, Gert, Garssen, Johan, and Mortaz, Esmaeil
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INTERFERON receptors , *TOLL-like receptor agonists , *SARS-CoV-2 , *TOLL-like receptors , *T cells , *COVID-19 , *MONONUCLEAR leukocytes - Abstract
Signaling by toll-like receptors (TLRs) initiates important immune responses against viral infection. The role of TLRs in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is not well elucidated. Thus, we investigated the interaction of TLRs agonists and SARS-COV-2 antigens with immune cells in vitro. 30 coronavirus disease 2019 (COVID-19) patients (15 severe and 15 moderate) and 10 age and sex-matched healthy control (HC) were enrolled. Peripheral blood mononuclear cells (PBMCs) were isolated and activated with TLR3, 7, 8, and 9 agonists, the spike protein (SP) of SARS-CoV-2, and the receptor binding domain (RBD) of SP. Frequencies of CD3+IFN-β+ T cells, and CD3+IFN-γ+ T cells were evaluated by flow cytometry. Interferon (IFN)-β gene expression was assessed by qRT-PCR. The frequency of CD3+IFN-β+ T cells was higher in PBMCs from moderate (p < 0.0001) and severe (p = 0.009) patients at baseline in comparison with HCs. The highest increase in the frequency of CD3+IFN-β+ T cells in cell from moderate patients was induced by TLR8 agonist and SP (p < 0.0001 for both) when compared to HC, while, the highest increase of the frequency of CD3+IFN-β+ T cells in sample of severe patients was seen with TLR8 and TLR7 agonists (both p = 0.002). The frequency of CD3+IFN-γ+ T cells was significantly increased upon stimulation with TLR agonists in cell from patients with moderate and severe COVID-19, compared with HC (all p < 0.01), except with TLR7 and TLR8 agonists. The TLR8 agonist did not significantly increase the frequency of CD3+IFN-γ+ T cells in PBMCs of severe patients, but did so in cells from patients with moderate disease (p = 0.01). Moreover, IFN-β gene expression was significantly upregulated in CD3+T cells from moderate (p < 0.0001) and severe (p = 0.002) COVID-19 patients, compared to HC after stimulation with the TLR8 agonist, while, stimulation of T cells with SP, significantly up-regulated IFN-β mRNA expression in cells from patients with moderate (p = 0.0003), but not severe disease. Stimulation of PBMCs from COVID-19 patients, especially patients with moderate disease, with TLR8 agonist and SP increased the frequency of IFN-β-producing T cells and IFN-β gene expression. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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8. Comparative evaluation of SARS-CoV-2 IgG assays against nucleocapsid and spike antigens.
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Rezaei, Mitra, Sadeghi, Mohammadhadi, Korourian, Alireza, Tabarsi, Payam, Porabdollah, Mihan, Askari, Elham, Mortaz, Esmaeil, Mahmoudi, Shima, Marjani, Majid, and Velayati, Ali Akbar
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SARS-CoV-2 , *REVERSE transcriptase polymerase chain reaction , *COVID-19 , *IMMUNOGLOBULIN G , *ANTIGENS , *ANTIBODY formation , *VIRAL antibodies - Abstract
BACKGROUND: There are few studies to compare antibody response against anti-spike (S) and anti- nucleoprotein (N) SARS-CoV-2. OBJECTIVE: The aim of this study was to evaluate the IgG antibody production against S and N antigens of the virus and their correlation with the time and severity of the disease. METHODS: The IgG antibodies against S and N antigens of SARS-CoV-2 in serum specimens of 72 symptomatic patients who tested real-time reverse transcription polymerase chain reaction positive for SARS-CoV-2 were detected using the ELISA technique. Different antibody response was compared and the correlation with the time from disease onset and the severity was evaluated. RESULTS: Forty-eight of 72 (67%) patients tested positive for anti-SARS-CoV-2 antibodies, while 24 (33%) did not have detectable antibodies. Comparison of antibody levels for N and S antibodies showed that they correlate with each other well (r = 0.81; P < 0.001). However, sensitivity of anti-S SARS-CoV-2 IgG and anti-N SARS-CoV-2 IgG was 30% and 60%, during the first 7 days after symptom onset (r = 0.53; P = 0.111), but increased to 73% and 68% at more than 1-week post symptom onset (r = 0.89, P = 0.111), respectively. Cases with positive IgG response showed a decreased CD8 + T cells percentage compared to the negative IgG groups (26 ± 14 vs. 58 ± 32, p = 0.066 in anti-N IgG group and 28 ± 15 vs. 60 ± 45, p = 0.004 in anti-S IgG group, respectively). CONCLUSION: Nearly one-third of the confirmed COVID-19 patients had negative serology results. Lower percent positivity at early time points after symptom onset (less than 1 week) was seen using anti-S SARS-COV-2 IgG kit compare to the anti-N SARS-CoV-2 IgG; therefore, clinicians should interpret negative serology results of especially anti-S SARS-CoV-2 IgG with caution. [ABSTRACT FROM AUTHOR]
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- 2021
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9. Update on Immunology of COVID-19 Disease and Potential Strategy for Controlling.
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Dezfuli, Neda K., Adcock, Ian M., Montazami, Nooshin, Mortaz, Esmaeil, and Velayati, Aliakbar
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COVID-19 , *SARS-CoV-2 , *ADULT respiratory distress syndrome , *CYTOKINE release syndrome , *SARS virus - Abstract
Coronavirus disease 2019 (COVID-19) is caused by a novel form of the coronavirus that caused severe acute respiratory syndrome (SARS). SARS-CoV-2 raised in China and has broadcast to 261 countries globally. SARS-CoV-2 a member of β-coronavirus family and has an almost matching genome sequence to a bat coronavirus, pointing to the bat as the natural host before it was transmitted to humans. SARS-CoV-2 uses the same receptor, angiotensinconverting enzyme 2 (ACE2) as that used by SARS-CoV and principally infects the respiratory tract. The clinical symptoms of COVID-19 patients include fever, cough and fatigue whilst small populations of patients have gastrointestinal symptoms. The old people and people with underlying metabolic and cardiovascular diseases are more affected to infection and have worse outcomes. These may be associated with acute respiratory distress syndrome (ARDS) and a cytokine storm. In this review, we discuss the pathogenesis and clinical characteristics of disease and the pharmacologic approaches that may control COVID-19. [ABSTRACT FROM AUTHOR]
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- 2020
10. Immunopathogenesis of Pneumonia in COVID-19.
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Alipoor, Shamila D., Jamaati, Hamidreza, Tabarsi, Payam, and Mortaz, Esmaeil
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COVID-19 , *MIDDLE East respiratory syndrome , *SARS-CoV-2 , *TUMOR necrosis factors , *SARS disease , *PULMONARY fibrosis - Published
- 2020
11. No clinical benefit of high dose corticosteroid administration in patients with COVID-19: A preliminary report of a randomized clinical trial.
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Jamaati, Hamidreza, Hashemian, Seyed MohammadReza, Farzanegan, Behrooz, Malekmohammad, Majid, Tabarsi, Payam, Marjani, Majid, Moniri, Afshin, Abtahian, Zahra, Haseli, Sara, Mortaz, Esmaeil, Dastan, Alireza, Mohamadnia, Abdolreza, Vahedi, Abdolbaset, Monjazebi, Fatemeh, Yassari, Fatemeh, Fadaeizadeh, Lida, Saffaei, Ali, and Dastan, Farzaneh
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CLINICAL trial registries , *COVID-19 , *CLINICAL trials , *CORTICOSTEROIDS , *COMPUTED tomography - Abstract
The aim of this study was to evaluate the clinical effects of dexamethasone administration in patients with mild to moderate acute respiratory distress syndrome (ARDS) due to coronavirus disease 2019 (COVID-19). The study included 50 patients who were randomly assigned to the dexamethasone group or control group. Dexamethasone was administered at a dose of 20 mg/day from day 1–5 and then at 10 mg/day from day 6–10. The need for invasive mechanical ventilation, death rate, duration of clinical improvement, length of hospital stay, and radiological changes in the computed tomography scan were assessed. The results revealed that 92% and 96% of patients in the dexamethasone and control groups, respectively, required noninvasive ventilation (P = 0.500). Among them, 52% and 44% of patients in the dexamethasone and control groups, respectively, required invasive mechanical ventilation (P = 0.389). At the end of the study, 64% of patients in the dexamethasone group and 60% of patients in the control group died (P = 0.500); the remaining patients were discharged from the hospital during the 28-day follow-up period. The median length of hospital stay was 11 days in the dexamethasone group and 6 days in the control group (P = 0.036) and the median length of hospital stay was 7 days in the dexamethasone group and 3 days in the control group (P < 0.001). No significant differences were observed in the other outcomes. This study showed that corticosteroid administration had no clinical benefit in patients with COVID-19-induced mild to moderate ARDS. [ABSTRACT FROM AUTHOR]
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- 2021
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