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Hyperbilirubinemia is the condition when bilirubin exceeds normal concentration in body (19.80 mg/mL in newborns and 1.19 × 10 -2  mg/mL in adults). Bilirubin encephalopathy in newborns may cause irreversible neurological disorders. Current methods for detection of bilirubin suffer from compromising accuracy. In the present work, bovine serum albumin stabilized copper nanocluster (BSA-CuNCs) was synthesized via a one pot microwave assisted method as a turn on detector for bilirubin. The synthesized BSA-CuNCs having size less than 4 nm, exhibited bright blue emission at 405 nm. Interestingly, no observable change in fluorescence emission was noticed over a wide pH range (1-11) or at high ionic conditions. However, the interaction of Fe 3+ with BSA-CuNCs induces quenching of fluorescence. Moreover, the fluorescence can be regained by the addition of bilirubin over other possible coexisting biomolecules. A good linearity was observed for BSA-CuNCs based turn on probe with a Limit of Detection (LoD) 6.62 nM. Furthermore, real sample analyses were carried out with human serum and urine which showed good recovery percentage., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Hurdles to develop point of care diagnostic methods restrict the translation of progress in the health care sector from bench side to bedside. In this article a simple, cost effective fluorescent as well as colorimetric nanosensor was developed for the early and easy detection of hyperbilirubinemia. A stable, water soluble bovine serum albumin stabilised copper nanocluster (BSA CuNC) was used as the fluorescent probe which exhibited strong blue emission (404nm) upon 330nm excitation. The fluorescence of the BSA CuNC can be effectively quenched by the addition of bilirubin by the formation of copper-bilirubin complex. Meanwhile the copper-bilirubin complex resulted in an observable colour change from pale violet to green facilitating colorimetric detection. The prepared sensor displayed good selectivity and sensitivity over other co-existing molecules, and can be used for quantifying bilirubin with a detection limit down to 257fM. Additionally, the as-prepared probe was coated on a paper strip to develop a portable paper strip sensor of bilirubin. Moreover, the method was successfully applied in real sample analysis and obtained promising result., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Lead halide perovskites are an advanced class of materials with high photoluminescence and optoelectronic properties. However their instability towards moisture and lead toxicity hinders their applications especially in biological sensing. Hence the need for novel lead free perovskite nanocrystals are of utmost important. Herein we synthesized a lead free, highly efficient, stable, cost effective Cs 2 ZnCl 4 perovskite nanocrystals (PNCs) and extends its application in biosensing. The PNCs exhibits bright blue fluorescence upon UV irradiation and was able to selectively detect bilirubin (BR) - a clinically important jaundice biomarker. The intense fluorescence emission of the sensor was completely quenched upon the addition of bilirubin. The sensor could detect bilirubin even in the presence of other interfering biomolecules with a good limit of detection of 26.10 nM. The sensor could successively detect BR in human serum samples. Furthermore, for the visual detection of bilirubin, a paper strip sensor was also designed making the sensor more economical and adaptable. [Display omitted] • Lead free Cs 2 ZnCl 4 Perovskite nanocrystals were synthesized as the fluorescent probe. • The fluorescence intensity of the probe was drastically quenched upon bilirubin addition. • The sensor displayed a good selectivity and sensitivity towards bilirubin over other coexisting biomolecules. • The sensor shows an accurate recovery percent when applied to human serum samples. • For rapid visual detection, a paper strip sensor was also developed. [ABSTRACT FROM AUTHOR]

Schematic Representation of developed fluorescent "Turn-off" sensor for the detection of bilirubin. [Display omitted] • Bright green Tb-DPA luminescent complex was designed. • Tb-DPA act as a luminescent probe for the detection of bilirubin. • Selectivity and sensitivity with other coexisting biomolecules were evaluated. • Real sample analysis is performed in human serum and urine samples and obtained satisfactory result. The sensitive and selective fluorescent probes for biomolecule detection have significant application in clinical diagnosis and bioanalysis. Hyperbilirubinemia is a disease condition in which the excessive or less production of bilirubin in blood resulting in jaundice. In this work, we have synthesised Terbium based bright green, fluorescent lanthanide complex (Tb-DPA) with emission at λ em = 544 nm for the detection of Bilirubin. A one-step synthesis approach was adopted with 2,6-pyridine dicarboxylic acid (DPA) as ligand and Sodium dodecyl sulphate (SDS) as surfactant. The green fluorescence of Tb-DPA was quenched with the addition of bilirubin due to the inner filter effect mechanism. The fluorescence intensity of Tb-DPA decreases as bilirubin concentration increases and a linear " turn-off " response is obtained. Even in presence of other coexisting biomolecules and ions, the Tb-DPA probe exhibited good selectivity and sensitivity for the detection of bilirubin. The limit of detection (LOD) of 2.6 mg/dL was obtained. Furthermore, the proposed fluorescent probe was applied in Human serum and urine samples with satisfactory recoveries in the range of 92–122 %. Based on the result obtained, it has probability to be used as a rapid detection method for bilirubin in biological samples at bedside. [ABSTRACT FROM AUTHOR]