1. Inhibitory effects of tricin derivative from Sasa albo-marginata on replication of human cytomegalovirus
- Author
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Akuzawa, Kazuhiko, Yamada, Rie, Li, Zhuan, Li, Ying, Sadanari, Hidetaka, Matsubara, Keiko, Watanabe, Kunitomo, Koketsu, Mamoru, Tuchida, Yuuzo, and Murayama, Tsugiya
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CYTOMEGALOVIRUSES , *FIBROBLASTS , *CELL lines , *WESTERN immunoblotting , *VIRAL replication , *PLAQUE assay technique , *PROSTAGLANDINS , *DNA polymerases - Abstract
Abstract: The anti-human cytomegalovirus (HCMV) activity of tricin (4′,5,7-trihydroxy-3′,5′-dimethoxyflavone), a derivative from Sasa albo-marginata, was studied in the human embryonic fibroblast cell line MRC-5. In a plaque assay, tricin and ganciclovir (GCV) showed concentration-dependent inhibitory properties from 0.05 to 3.6μM and 0.01 to 1.0μM, respectively. Tricin had no virucidal effects on cell-free HCMV. Treatment with tricin 1h before, or 1h or 3h after viral infection significantly suppressed HCMV replication. Moreover, tricin inhibited the expression of immediate early (IE) 2 mRNA and DNA polymerase (UL54) mRNA in HCMV-infected cells. Western blot analysis also demonstrated that tricin decreased the expression of IE antigen (especially IE2) and cyclooxygenase 2 (COX-2) expression in HCMV-infected cells. In the presence of tricin, prostaglandin E2 (PGE2) accumulation by HCMV infection was completely inhibited. These results suggest that tricin is a novel compound with potential COX inhibitor-dependent anti-HCMV activity. [Copyright &y& Elsevier]
- Published
- 2011
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