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Simple Summary: In modern aquaculture, fish have been subjected to intensive stressful conditions, which threaten their growth rate and increase their susceptibility to bacterial diseases. Innovative and sustainable production strategies in aquaculture have encouraged the utilization of plant-derived bioactive molecules (phytogenics) in fish diets, especially when they were used in a unique blend offering enumerable growth and health benefits. Therefore, our research was conducted to uncover the potential nutritional and immunological impacts of thymol (Thy) and/or thymoquinone (ThQ) with a disease shielding effect against Aeromonas sobria. Herein, the integration of dietary Thy and ThQ provided an efficient way to improve fish growth-related parameters and muscle antioxidant capacity. Moreover, we presented evidence for the unique immune-stimulating role of a Thy and ThQ combination with a successful forefront defense against A. sobria experimental infection in Nile tilapia. Our key findings motived the application of a dietary blend comprising Thy and ThQ as a functional feed that fights the challenges facing the aquaculture industry with maximized fish productivity. Plant-derived bioactive compounds with promising nutritional and therapeutic attributes (phytogenics) are among the top priorities in the aquaculture sector. Therefore, the impact of thymol (Thy) and/or thymoquinone (ThQ) on the growth, immune response antioxidant capacity, and Aeromonas sobria (A. sobria) resistance of Nile tilapia was investigated. Four fish groups were fed a control diet and three basal diets supplemented with 200 mg/kg diet of Thy or ThQ and a blend of both Thy and ThQ at a level of 200 mg/kg diet each. At the end of the feeding trial (12 weeks), the tilapias were challenged intraperitoneally with virulent A. sobria (2.5 × 108 CFU/mL) harboring aerolysin (aero) and hemolysin (hly) genes. The results revealed that tilapias fed diets fortified with a combination of Thy and ThQ displayed significantly enhanced growth rate and feed conversion ratio. Notably, the expression of the genes encoding digestive enzymes (pepsinogen, chymotrypsinogen, α-amylase and lipase) and muscle and intestinal antioxidant enzymes (glutathione peroxidase, catalase and superoxide dismutase) was significantly upregulated in Thy/ThQ-fed fish. An excessive inflammatory response was subsided more prominently in the group administrated Thy/ThQ as supported by the downregulation of il-β, il-6 and il-8 genes and in contrast, the upregulation of the anti-inflammatory il-10 gene. Remarkably, dietary inclusion of Thy/ThQ augmented the expression of autophagy-related genes, whilst it downregulated that of mtor gene improving the autophagy process. Furthermore, Thy/ThQ protective effect against A. sobria was evidenced via downregulating the expression of its aero and hly virulence genes with higher fish survival rates. Overall, the current study encouraged the inclusion of Thy/ThQ in fish diets to boost their growth rates, promote digestive and antioxidant genes expression, improve their immune responses and provide defense against A. sorbia infections with great economic benefits. [ABSTRACT FROM AUTHOR]

The limit of tolerance to salinity in freshwater fish has become an important environmental factor influencing growth performance. Therefore, this study aimed to explore the impact of different levels of salinity on behavioural responses, growth performance, haematological, oxidative and biochemical stress parameters, in addition to immune parameters of African catfish. Three‐hundred and Sixty juvenile African catfish were randomly distributed into six groups and exposed to different salinity levels (0, 4, 8, 12, 16 and 20 ppt) with three replicate tanks per treatment group; each tank contained 20 individuals for 42 days. Behavioural responses of fish were monitored during the experimental period. Growth performance, haematological, oxidative and biochemical stress parameters, in addition to immune parameters and osmotic pressure, were also determined at the end of the treatment period. Results revealed that the proportion of fish performing abnormal stress behavioural responses increased directly to the salinity level. In contrast, the survival rate and growth performance were decreased with high feed conversion at the higher salinities. Inconsistent, the haematological, oxidative, biochemical, immune and osmotic pressure parameters were altered gradually with the increase in the salinity level. Therefore, salinity level could influence the African catfish's behaviour, survival and growth when they exceed the tolerance limit. [ABSTRACT FROM AUTHOR]

The poultry farms need a safe and effective alternative for antibiotics that can counteract the negative impacts of necrotic enteritis (NE), which causes severe mortalities and economic losses. The current study was aimed to examine the influence of antibiotic (Flagymox) and the microbial‐based administration on carcass traits in Clostridium(C.)perfringens‐infected Cobb and Arbor Acres broilers. A total number of 360 Cobb and Arbor Acres broiler chicks (180 numbers per breed) were allocated to four groups; negative control group (without any treatments); positive control group (administration of C. perfringens at the rate of 1 × 109cfu/bird via crop gavage twice daily from day 16 to 18 post‐hatch); C. perfringens challenge plus antibiotic (Flagymox®) group, and Clostridiumperfringens challenge plus microbial‐based treatment (Big‐lactoα®) group. The results indicated that the Flagymox and Big‐lactoα treated Cobb breed group achieved a significant increase in their body weight (BW) than the positive control group at the third week post‐infection. Also, the Arbor Acres breed gained significantly higher weight compared to the Cobb breed at the third week. Total weight gain (TWG) from 0 to the fifth week in the Cobb and Arbor Acres breeds were higher in the groups treated with Flagymox and Big‐lactoα compared to the birds challenged with C. perfringens without any treatment, thus, increasing the total return (TR) in the treated groups. Economic efficiency showed no significant differences (p <.05) between the treatment groups of both the breeds. Although the treatment cost of Flagymox is higher than the microbial‐based treatment (0.86 versus 0.35 LE), there were no mortalities reported in the microbial‐based groups in both the breeds resulting in significantly low losses compared to the Flagymox treated groups. The groups treated with the microbial‐based products in both breeds were superior in dressing percentage (75.16 and 77.06% for Cobb and Arbor Acres, respectively) compared to that of the other groups. In conclusion, microbial‐based therapy improved the growth rate, carcass traits, survival rate, and economic efficiency in necrotic enteritis induced in Cobb and Arbor Acres broilers. [ABSTRACT FROM AUTHOR]

Simple Summary: Despite the beneficial role of antibiotics in reducing bacterial infection in rabbits, there is an indirect harmful influence on human health. Thus, replacing antibiotics with friendly alternatives is a suitable strategy to protect the performance and welfare of rabbits. This study aimed at comparing the effects of including dietary egg lysozyme and zinc bacitracin antibiotic (ZnB) on the productivity and health conditions of rabbits. The results show a marked enhancement of the growth performance, antibacterial capacity, blood health, and antioxidative status in rabbits treated with egg lysozyme compared with those treated with ZnB. Thus, using egg lysozyme is recommended to replace the usage of ZnB in rabbit production. Dietary egg lysozyme has beneficial roles in the growth performance and health conditions of animals. The study was performed using 90 multicolored rabbits in three groups (each replicate with thirty rabbits). In the control group, rabbits were fed a diet without zinc bacitracin (ZnB) or egg lysozyme, while the second and third groups were treated with ZnB and lysozyme additive at 100 mg/kg, respectively. After eight weeks, the final weight and body weight gain (BWG) of rabbits fed dietary egg lysozyme and ZnB additives were meaningfully increased (p < 0.05). Nevertheless, the feed conversion ratio (FCR) was markedly decreased by dietary egg lysozyme and ZnB (p < 0.05). Interestingly, dietary egg lysozyme resulted in higher final weight and BWG and lower FCR than rabbits treated with ZnB (p < 0.05). Rabbits treated with egg lysozyme and ZnB additives had markedly lower populations of Clostridium spp. and Escherichia coli (p < 0.05) compared with the control. However, the counts of Lactobacillus and total bacteria were meaningfully increased in the the intestines of rabbits treated with egg lysozyme and ZnB (p < 0.05). The blood total protein and globulin of rabbits fed dietary egg lysozyme and ZnB additives were meaningfully increased (p < 0.05). Blood creatinine was significantly lowered by dietary egg lysozyme compared with the control and ZnB-treated rabbits (p < 0.05). The levels of blood urea, ALT, and AST were markedly lowered (p < 0.05) by dietary egg lysozyme and ZnB. The gene expressions of superoxide dismutase 1 (SOD1) and glutathione peroxidase (GPX) in the liver of rabbits fed dietary egg lysozyme and ZnB additives were markedly upregulated (p < 0.05) compared with the control. Dietary egg lysozyme resulted in higher expression of SOD1 and GPX genes than rabbits treated with ZnB (p < 0.05). In conclusion, the inclusion of egg lysozyme could replace the inclusion of ZnB in the diets of rabbits. [ABSTRACT FROM AUTHOR]

Simple Summary: Corn silk meal (CSM), the outer thread-like material found under corn husks, is a by-product of corn cultivation, which has been documented as a traditional medicine with multiple benefits for human health. Broilers cannot produce the enzymes needed to hydrolyze non-starch polysaccharide (NSP), which has been found to increase gut viscosity, and reduce the digestion and utilization of nutrients, thereby resulting in poor animal performance. The objective of this study was to examine the effects of diets supplemented with CSM and NSP on growth performance, blood biochemistry, immunological and growth-related gene expression in broiler chickens. A total of 270 broiler chickens were divided into six experimental groups: (1) basal diets (BD) as control; (2) BD supplemented with 0.5 g/kg feed NSP enzyme; (3) and (4) fed a diet containing 40 and 80 kg/ton of CSM; (5) and (6) fed a diet containing 40 and 80 kg/ton CSM and supplemented with 0.5 g/kg NSP enzyme. The results showed that the dietary inclusion of CSM with the NSP enzyme can improve growth performance, intestinal histopathology parameters, modify plasma lipids, and enhance immune response in broilers. The objective of this study was to examine the effects of diets supplemented with corn silk meal (CSM) and non-starch polysaccharide (NSP) enzyme on growth performance, blood biochemistry, immunological response, and growth-related gene expression in broiler chickens. A total of 270 broiler chickens were divided into six experimental groups: (1) basal diets (BD) as control; (2) BD supplemented with 0.5 g/kg feed NSP enzyme; the other four groups are CSM diets as following; (3) and (4) fed diet contain 40 and 80 kg/ton of CSM; (5) and (6) fed diet contain 40 and 80 kg/ton CSM and supplemented with 0.5 g/kg NSP enzyme. Body weight gain (BWG), feed conversion ratio (FCR), protein retention and fiber digestibility were synergistically improved (p < 0.05) when fed CSM supplemented with NSP enzyme. Moreover, a synergistic decrease (p < 0.05) in the serum glucose and total cholesterol were found. Immune organ weights and Newcastle disease virus titers were increased with CSM diets. Interestingly, the relative mRNAs of the growth hormone receptor (GHR) and insulin growth factor (IGF) were increased (p < 0.05) with the CSM and NSP enzyme: the relative mRNA expressions of cholecystokinin (CCK) and leptin were decreased by feeding CSM diets with the NSP enzyme. It could be concluded that the dietary inclusion of CSM with the NSP enzyme might improve growth performance, modify plasma lipids, and enhance immune response in broilers. [ABSTRACT FROM AUTHOR]