1. Micro RNA-101b attenuates cardiomyocyte hypertrophy by inhibiting protein kinase C epsilon signaling.
- Author
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Lee, Jong Sub, Yang, Dong Kwon, Park, Jei Hyoung, Kim, Jin Ock, Park, Woo Jin, Cho, Chunghee, and Kim, Do Han
- Subjects
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MICRORNA , *HEART cells , *HYPERTROPHY , *PROTEIN kinase C , *CELLULAR signal transduction , *VENTRICULAR remodeling , *CARDIAC hypertrophy , *REVERSE transcriptase polymerase chain reaction - Abstract
Previously, a surgical regression model identified micro RNA-101b ( miR-101b) as a potential inhibitor of cardiac hypertrophy. Here, we investigated the antihypertrophic mechanism of miR-101b using neonatal rat ventricular myocytes. miR-101b markedly suppressed agonist-induced cardiac hypertrophy as shown by cell size and fetal gene expression. By systems biology approaches, we identified protein kinase C epsilon ( PKCε) as the major target of miR-101b. Our results from qRT- PCR, western blot, and luciferase reporter assays confirm that PKCε is a direct target of miR-101b. In addition, we found that effectors downstream of PKCε (p- AKT, p- ERK1/2, p- NFAT, and p- GSK3β) are also affected by miR-101b. Our study reveals a novel inhibitory mechanism for miR-101b as a negative regulator of cardiac hypertrophy. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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