1. CFTR Deletion in Mouse Testis Induces VDAC1 Mediated Inflammatory Pathway Critical for Spermatogenesis
- Author
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Xie Jiang, Sun Huaqin, Xu Wenming, Liao Huijuan, Chen Yan, Qin Lang, and Yang Ming
- Subjects
0301 basic medicine ,Male ,Physiology ,lcsh:Medicine ,Cystic Fibrosis Transmembrane Conductance Regulator ,RNA-binding proteins ,Cystic fibrosis ,Biochemistry ,Heat Shock Response ,Epithelium ,Reproductive Physiology ,Animal Cells ,Immune Physiology ,Testis ,Medicine and Health Sciences ,Cell Cycle and Cell Division ,lcsh:Science ,Energy-Producing Organelles ,Cellular Stress Responses ,Regulation of gene expression ,Innate Immune System ,Multidisciplinary ,Chromosome Biology ,NF-kappa B ,respiratory system ,Sertoli cell ,Cell biology ,Mitochondria ,Meiosis ,medicine.anatomical_structure ,Cell Processes ,Cytokines ,Signal transduction ,Cellular Types ,Anatomy ,Cellular Structures and Organelles ,VDAC1 ,Germ cell ,Research Article ,Signal Transduction ,medicine.medical_specialty ,congenital, hereditary, and neonatal diseases and abnormalities ,Immunology ,Biology ,Bioenergetics ,Research and Analysis Methods ,03 medical and health sciences ,Internal medicine ,medicine ,Animals ,Mice, Inbred CFTR ,Heat shock ,Spermatogenesis ,Immunohistochemistry Techniques ,Inflammation ,Sertoli Cells ,Endoplasmic reticulum ,Voltage-Dependent Anion Channel 1 ,lcsh:R ,Biology and Life Sciences ,Proteins ,Epithelial Cells ,Cell Biology ,Molecular Development ,medicine.disease ,Histochemistry and Cytochemistry Techniques ,030104 developmental biology ,Endocrinology ,Germ Cells ,Biological Tissue ,Gene Expression Regulation ,Immune System ,Immunologic Techniques ,lcsh:Q ,Gene Deletion ,Developmental Biology - Abstract
Cystic fibrosis is the most common genetic disease among Caucasians and affects tissues including lung, pancreas and reproductive tracts. It has been shown that Endoplasmic Reticulum (ER) stress and heat shock response are two major deregulated functional modules related to CFTR dysfunction. To identify the impact of CFTR deletion during spermatogenesis, we examined the expression of spermiogenesis-related genes in the testis of CFTR mutant mice (CF mice). We confirmed expression changes of MSY2, a germ cell specific RNA binding protein, resulting from deletion of CFTR in testis. Furthermore, real time PCR and Western blot results showed that an inflammatory response was activated in CF mice testis, as reflected by the altered expression of cytokines. We demonstrate for the first time that expression of MSY2 is decreased in CF mice. Our results suggest that CFTR deletion in testis influences inflammatory responses and these features are likely to be due to the unique environment of the seminiferous tubule during the spermatogenesis process. The current study also suggests avenues to understand the pathophysiology of CFTR during spermatogenesis and provides targets for the possible treatment of CFTR-related infertility.
- Published
- 2016