1. Application of UV spectrophotometric method for easy and rapid estimation of sulfasalazine in pharmaceutical formulation (suspension).
- Author
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Mehmood, Yasir, Saleem, Noviara, Syed, Muhammad Ali, and Hanif, Sana
- Subjects
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ULTRAVIOLET spectrophotometry , *SOLUTION (Chemistry) , *SUSPENSIONS (Chemistry) , *BEER-Lambert law ,SULFONAMIDE drugs - Abstract
Introduction: The present research work is about the development of a simple, economic, accurate, quick and reproducible UV spectrophotometric method for sulfasalazine (SFZ) estimation in the pharmaceutical formulation (suspension) and also when it is in the bulk. Materials and Methods: Water and methanol was used in proportion of 1:1 for the preparation of stock solution. Different solutions of SFZ were prepared by diluting the stock solution with water. Results: The solution was prepared and determine at wavelength λmax 359 nm. The drug was determined at maximum wavelength (λmax 359 nm). When the drug concentration is in the range of 2-20 μg/ml it obeys Beers law with line equation y=0.073x+0.003 and correlation coefficient of 0.992. Results obtained were validated statistically and by recovery study method. Conclusion: The result of analysis was validated according to ICH guidelines and this proposed method can be used for the routine analysis of sulfasalazine suspension formulation. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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