1. Molecular epidemiology of rabies in Guangxi Province, south of China
- Author
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Liu, Qi, Xiong, Yi, Luo, Ting Rong, Wei, You-Chuan, Nan, Song-Jian, Liu, Fang, Pan, Yan, Feng, Li, Zhu, Wei, Liu, Ke, Guo, Jian-Gang, and Li, Hua-Ming
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MEDICAL research , *RABIES , *VIRUS diseases , *EPIDEMIOLOGY - Abstract
Abstract: Background: Surveillance data for rabies in Guangxi Province in China showed that human rabies cases have gradually increased since 1996. Objective: To evaluate the epidemiology of rabies at the molecular level and provide suggestions for effective prevention of rabies in Guangxi. Study design: Since 2000, 1569 brains from suspected rabid animals were collected from different areas of Guangxi. Rabies virus was isolated from 42 samples. RT-PCR was used to amplify a 455 nucleotide segment of the 3′-terminal of the N gene. The sequencing data from that segment was used for phylogenetic analysis. Results: Nucleotide homology comparisons and phylogenetic tree analysis based on this sequence indicated that all the rabies virus isolates from Guangxi belonged to genotype 1 and could be divided into four groups. Groups I, II and IV included 23, 10 and 8 isolates, respectively. These had nucleotide homologies of 97.1–100%, 98.2–100% and 99.1–99.6%, respectively. Only the GXN119 strain belonged to group III. Group I had two group-specific mutations: T90N and E110D. Group II had one group-specific mutation of T42S. Conclusions: This study showed that rabies virus isolates from Guangxi have a close genetic relationship and topographical distribution. [Copyright &y& Elsevier]
- Published
- 2007
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