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Pork quality is determined by several attributes, among which odor and taste are the utmost significant. Therefore, this study was aimed to assess the effects of boar odor hormone concentration on the quality traits and sensory acceptability of pork. A total twenty-six (26) non-castrated 3-way crossbred (Landrace × Yorkshire × Duroc) pigs were selected with an average body weight (ABW) 115.6 kg before to slaughter. The three treatment groups (low, medium and high) were divided according to the androstenone concentration. In experiment 1, for meat quality traits carcass was selected based on androstenone concentration: low (LC, 0.64–0.69 μg/g, n = 9), medium (MC, 0.70–0.99 μg/g, n = 7) and high (HC, 1.00–1.69 μg/g, n = 10). In experiment 2, for sensory evaluation carcasses were also selected based on the abovementioned conditions. Results revealed that androstenone concentration not effect on proximate components, meat quality traits and fatty acids except palmitoleic acid. Sensory evaluation data showed that boar taint and meat boar taint were significantly increased in a concentration-dependent manner from low to high, whereas, gravy and meat flavor preference were significantly increased in LC group than HC group. In addition, correlation analysis showed that boar taint and meat boar taint were positively, and gravy and meat flavor preference were negatively correlated with boar taint hormones. In essence, our findings indicate that androstenone concentration had no effect on meat qualities, but a high concentration of androstenone had a negative effect on the sensory characteristics in uncastrated pigs.

We aimed to determine the effects of oleic acid (OA) and palmitic acid (PA), alone or in combination, on proliferation, differentiation, triacylglycerol (TAG) content, and gene expression in porcine muscle satellite cells (PMSCs). Results revealed that OA-alone- and PA + OA-treated PMSCs showed significantly increased viability than those in the control or PA-alone-treated groups. No significant effects on apoptosis were observed in all three treatments, whereas necrosis was significantly lower in OA-alone- and PA + OA-treated groups than in the control and PA-alone-treated groups. Myotube formation significantly increased in OA-alone and PA + OA-treated PMSCs than in the control and PA-alone-treated PMSCs. mRNA expression of the myogenesis-related genes MyoD1 and MyoG and of the adipogenesis-related genes PPARα, C/EBPα, PLIN1, FABP4, and FAS was significantly upregulated in OA-alone- and PA + OA-treated cells compared to control and PA-alone-treated cells, consistent with immunoblotting results for MyoD1 and MyoG. Supplementation of unsaturated fatty acid (OA) with/without saturated fatty acid (PA) significantly stimulated TAG accumulation in treated cells compared to the control and PA-alone-treated PMSCs. These results indicate that OA (alone and with PA) promotes proliferation by inhibiting necrosis and promoting myotube formation and TAG accumulation, likely upregulating myogenesis- and adipogenesis-related gene expression by modulating the effects of PA in PMSCs.

Quail housing and diet significantly impact egg yield and quality. This study assessed the impact of diets and housing arrangements on Japanese quail's egg production, egg index, and quality. In two trials, birds were reared in cages and on the floor with a commercial layer diet (CLD), and an experimental diet (ED) for 32 weeks. Compared to floor-reared birds, cage birds achieved 50% egg production and sexual maturity first. With dietary effects, the CLD diet showed similar results. Furthermore, their feed efficiency, hen house egg production (HHEP), and hen day egg production (HDEP) were significantly higher in cage birds that consumed CLD. Bird livability was unaffected by the housing system, while birds fed CLD had longer lifespans. The housing system had no discernible effects on egg dry matter (DM), crude protein (CP), crude fat (CF), or ash percentage. Nevertheless, the egg albumen's DM, CP, ash%, yolk's DM, CP, and CF were greater in the birds fed CLD. Barring shell thickness, weight, and Haugh unit, birds raised in cages exhibited higher egg exterior index values. Besides the yolk ratio, yolk index, and albumen weight ratio, birds given CLD exhibited enhanced egg interior quality. Ultimately, the most optimal egg production performance, improved egg quality, and prolonged lifespan of Japanese quail were obtained with cage raising and feeding practices utilizing CLD.

Co-culture is mimicked in vivo condition, which is influenced by adjacent cells. This study aimed to determine different myogenic genes in mono- and co-culture of satellite cells and fibroblasts. Fibroblasts and satellite cells were isolated from the piglet’s thigh muscles. The expression of PAX-7, MyoD, MyoG, and PAX-3 was downregulated in 24 h co-culture satellite cells than in monoculture. The expression of Myf-5 and CAV-1 was upregulated in 24 h co-culture satellite cells than in 24 h monoculture. The expression of PAX-7, MyoD, and Myf-5 was downregulated in 24 and 48 h co-culture fibroblasts than 24 h monoculture. The expression of MyoG and PAX-3 was upregulated in 24 and 48 h co-culture than 48 h monoculture fibroblasts. The Cyto-C was downregulated in 24 and 48 h co-culture fibroblast than 24 h monoculture. The expression of MyoG and CAV-1 was upregulated in 48 h incubation period co-culture fibroblasts than 24 h monoculture. The MyoD and CAV-1 expression were upregulated in 24 h co-culture than 24 h satellite cells and fibroblasts. The Bcl-2 and BAX were downregulated in 48 h co-culture than 24 h. This result recommended that the satellite cells and fibroblasts interaction enhance the myogenesis marker expression without cellular morphology change.

Heat stress inhibits cell proliferation as well as animal production. Here, we aimed to demonstrate that 9-mer disulfide dimer peptide (CopA3) supplementation stabilizes porcine muscle satellite cell (PMSC) proliferation and heat shock protein (HSP) expression at different temperatures. Therefore, we investigated the beneficial effects of CopA3 on PMSCs at three different temperatures (37, 39, and 41 °C). Based on temperature and CopA3 treatment, PMSCs were divided into six different groups including treatment and control groups for each temperature. Cell viability was highest with 10 µg/mL CopA3 and decreased as the concentration increased in a dose-dependent manner. CopA3 significantly increased the cell viability at all temperatures at 24 and 48 h. It significantly decreased apoptosis compared to that in the untreated groups. In addition, it decreased the apoptosis-related protein, Bcl-2-associated X (BAX), expression at 41 °C. Notably, temperature and CopA3 had no effects on the apoptosis-related protein, caspase 3. Expression levels of HSP40, HSP70, and HSP90 were significantly upregulated, whereas those of HSP47 and HSP60 were not affected by temperature changes. Except HSP90, CopA3 did not cause temperature-dependent changes in protein expression. Therefore, CopA3 promotes cell proliferation, inhibits apoptosis, and maintains stable HSP expression, thereby enhancing the heat-stress-tolerance capacity of PMSCs.

Osteolytic lesions (OL) are frequent in multiple myeloma (MM), but are poorly understood. Here, the authors characterise OLs in MM patient samples using single-cell RNA-seq, revealing genes that are specifically regulated in OL compared to random bone marrow aspirates and that reflect the response to induction therapy.

Exploring 2D van der Waals (vdW) materials is of great importance to the various optoelectronics‐based applications. Herein, mono‐ and few‐layer nanoflakes of a novel 2D vdW MgTeMoO6 are prepared by facile mechanical exfoliation method. These nanoflakes remain highly stable when being exposed in air even for 1 month, which is unique compared to most of 2D materials. Temperature‐dependent Raman spectroscopy reveals that 2D MgTeMoO6 nanosheets exhibit stronger interlayer interaction than that of graphene. In situ, angle‐resolved polarized Raman spectroscopy investigation show evidences for the anisotropic photo–phonon interactions. Notably, strong in‐plane anisotropy for the second harmonic generation (SHG) independent of layer number is observed for 2D MgTeMoO6, making 2D MgTeMoO6 promising in polarization‐sensitive optoelectronic applications. [ABSTRACT FROM AUTHOR]

BackgroundRepetitive transcranial magnetic stimulation (rTMS) is a novel treatment for smoking cessation and delay discounting rate is novel therapeutic target. Research to determine optimal therapeutic targets and dosing parameters for long-term smoking cessation is needed. Due to potential biases and confounds introduced by the COVID-19 pandemic, we report preliminary results from an ongoing study among participants who reached study end prior to the pandemic.MethodsIn a 3 × 2 randomized factorial design, participants (n = 23) received 900 pulses of 20 Hz rTMS to the left dorsolateral prefrontal cortex (PFC) in one of three Durations (8, 12, or 16 days of stimulation) and two Intensities (1 or 2 sessions per day). We examined direction and magnitude of the effect sizes on latency to relapse, 6-month point-prevalence abstinence rates, research burden, and delay discounting rates.ResultsA large effect size was found for Duration and a medium for Intensity for latency to relapse. Increasing Duration increased the odds of abstinence 7–8-fold while increasing Intensity doubled the odds of abstinence. A large effect size was found for Duration, a small for Intensity for delay discounting rate. Increasing Duration and Intensity had a small effect on participant burden.ConclusionFindings provide preliminary support for delay discounting as a therapeutic target and for increasing Duration and Intensity to achieve larger effect sizes for long-term smoking cessation and will provide a pre-pandemic comparison for data collected during the pandemic.Clinical Trial Registration[www.ClinicalTrials.gov], identifier [NCT03865472].

The impact of Gestalt theoryon the photographic art image CompositionExpressive painting movement is a German and French for more points affecting the genesis and evolution of art, artists have called to resist the control of art in academic templates and obtain their freedom to express their feelings and self encloses herewith information and access to these feelings rather than show The external appearance of the subjects, or emotions. The research aims to determine the impact of the theory of Gestalt on the path of the photographic art movement, and its role in the evolution of the concepts of photography in the light of modern art schools and trends in the world. And prove that its principles are still valid in contemporary photographic art.The research problem is What are the changes brought about by Gestalt theories, ideas in the design of contemporary photo? And how it influenced the artistic vision of photography? Gestalt principles are in Visual perception are as follows:1. the ground Figure 2.Similarity 3-Proximity 4. Common region5. Continuity 6. Closure7. Focal Point-After completion of the research, the results can be formulated in the following points:- Gestalt principles adherents ideas tended to devise new forms of photographic compositions. - the principles Gestalt credit for laying the foundations for real photographic composition to become more nuanced compositions both for each component or as a whole.- Gestalt visions were formed a new photographic image are a no longer mere transfer of reality but a tool of creativity to the photographic artist, which has continued to this day and have become higher and improved production capability with the development of digital technologies and software image processors to produce photographically creative.The search ended for the following recommendations- Further studies and research for scholars and researchers in the field of professional photography ideas for principles of Gestalt.- the teaching principles of Gestalt within their curriculum in design at Department at the Faculty of applied arts Helwan University.- The photographers are working in the field of interest to study Gestalt principles to develop their creative and professional specializations.

This study was to investigate physicochemical characteristics, fatty acids, minerals and hematological alterations in broiler chickens artificially infected with 1 × 104 sporulated oocysts of Eimeria tenella. Results revealed that pH values were decreased (p ˂ .05) in the infected group (IG) at 7 and 9 days post infection (dpi) than those at 5 dpi, although no significant differences between the non-infected group (NIG) and IG. Infection with E. tenella did not affect meat colour parameters except that redness was lower (p ˂ .05) than that in the NIG. Cooking loss was decreased (p ˂ .05) in NIG and IG at 7 and 9 dpi than that at 5 dpi. Thiobarbituric acid reactive substance (TBARS) value was increased (p ˂ .05) in IG at 7 dpi than 5 or 9 dpi and NIG (p ˂ .01). Total protein (TP) and total cholesterol (TC) were significantly lowered in IG at 5 dpi. Other blood parameter results showed decreases of glucose (GLU), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL) in infected chicken at 7 dpi compared to those in the NIG without significance. However, albumin (ALB) level was decreased (p ˂ .01) in the IG compared with NIG at 5 dpi. Levels of aspartate aminotransferase (AST) were lowered (p < .05) in the IG than NIG at 7 dpi. Mineral contents of Ca, Mg, Cu, and Zn were lowered (p ˂ .01) in the IG than those in the NIG at 7 dpi. Based on these results indicate that a low dose of E. tenella has no impact on meat quality traits, fatty acids although it can slightly affect blood parameters and mineral contents.HIGHLIGHTS The low dose of Eimeria tenella has no impact on meat quality traits and fatty acids of broiler meat. A significant increase in some blood parameter is observed in infected broiler chickens at 9 dpi. Mineral contents of Ca, Mg, Cu and Zn decreases in infected broiler meat at 7 dpi.

Abstract Background Despite the considerable success of comprehensive tobacco control efforts, tobacco use remains one of the greatest preventable causes of death and disease today. Over half of all smokers in the US make quit attempts every year, but over 90% relapse within 12 months, choosing the immediate reinforcement of smoking over the long-term benefits of quitting. Conceptual and empirical evidence supports continued investigation of high frequency repetitive transcranial magnetic stimulation (rTMS) of the left dorsolateral prefrontal cortex in reducing relapse and decreasing cigarette consumption. While this evidence is compelling, an optimal dosing strategy must be determined before a long-term efficacy trial can be conducted. The goal of this study is to determine a dosing strategy for 20 Hz rTMS that will produce the best long-term abstinence outcomes with the fewest undesirable effects. Methods This is a fully crossed, double-blinded, sham-controlled, 3x2x2 randomized factorial study. The three factors are duration (stimulation days: 8, 12, and 16); intensity (900 or 1800 pulses per day); and sham control. Participants (n = 258) will consist of adults (18–65) who are motivated to quit smoking cigarettes and who will be followed for 6 months post-quit. Outcomes include latency to relapse, point prevalence abstinence rates, delay discounting rates, cognitive-behavioral skills acquisition, and multiple measures of potential undesirable effects that impact participant compliance. Discussion This study integrates existing theoretical concepts and methodologies from neuropsychology, behavioral economics, brain stimulation, clinical psychology, and the evidence-based treatment of tobacco dependence in the development of a promising and innovative approach to treat tobacco dependence. This study will establish an optimal dosing regimen for efficacy testing. Findings are expected to have a significant influence on advancing this approach as well as informing future research on clinical approaches that combine rTMS with other evidence-based treatments for tobacco dependence and perhaps other addictions. Trial registration Clinical Trials NCT03865472 (retrospectively registered). The first participant was fully enrolled on November 26, 2018. Registration was posted on March 7, 2019.

ABSTRACT: This study determined the relationship between inflammation and gluconeogenesis level in broilers in different durations of heat stress. A total of 240 Ross 308 broilers were offered control and heat stress temperature from 21 to 35 d post-hatch, each experimental group had 8 replications, and each replication obtained 15 broilers. The temperature in the control (Ctrl) group and heat stress group were maintained at 24 ± 1°C and 34 ± 1°C, respectively throughout the experimental period. Based on the duration of heat stress, the heat stress group was divided into 2 subgroups, like, 7-d heat stress (28-day-old broiler) designated ST group and 14-d heat stress (35-day-old broiler) designated the LT group. The ad libitum commercial feed and fresh water were provided to all experimental broilers during the experiment. The growth performance of experimental broilers was calculated at 35 d. However, the liver and blood samples were collected from the Ctrl group in 21 d, as well as these samples were collected from the heat stress ST and LT groups in 28-d and 35-d, respectively. Obvious gene expression of immunity, gluconeogenesis, glycogenolysis, and glycogenesis, as well as glucose-6-phosphate dehydrogenase and adenosine triphosphate was determined in the liver sample. The blood glucose concentration and histopathology of the liver was also examined in the different grouped broilers. Body weight, weight gain, and feed intake significantly decreased in the 35-d heat stress group than the Ctrl group. However, the feed conversion ratio increased at the 35-d heat stress group than the Ctrl group. The amount of glucose-6-phosphate dehydrogenase was significantly higher in ST and LT groups than Ctrl, whereas the blood glucose level was downregulated in the LT group. The amount of adenosine triphosphate was significantly decreased in the LT group than the Ctrl and ST groups. Heat stress acts as an impediment to the general relation between gluconeogenesis and immunity, as well as changes cellular structure. This experiment contributed to the establishment of a relationship between gluconeogenesis and immunity, which affects the growth performance of broilers during heat stress.

Most critics consider Contemporary Eric Johansson One of the greatest photographers of the 21st century. To the diversity of the methods of its photographic visual formulations, Despite his intellectual affiliation to the Surrealist school, he has developed his direction. Eric system In the production of a picture based on the idea of a composite of more than one topic and merged them creatively to appear as an integrated unit, named Realistic surrealism finally.Many equipment and tools must be available to be used in the production of photographs belonging to the real-life and the equipment for the general atmosphere designed for it, accessories and Special effects. Critics and theoreticians of art are classified Eric Johansson as a surreal and realistic because its visual treatment of subjects was not as classical surreal, abstract, or cubism, but it tends to use realistic forms that are one of the most distinctive features For photography.Research problem:The research problem is the central question:What is the impact of Eric Johansson work in Photographic Art in General and the surreal movement Special?A range of research questions and queries have emerged from this question, which formulated as follows: The Research Questions:1- Why do most critics consider Eric Johansson one of the greatest photographers of the 21st century?2- What is the limitation of technical trends in realism?3- Why did the critics and theorists classify the works of art Eric Johansson as surreal realistic?4- What equipment and tools should be available to be used in the production of photographic images belonging to Eric’s work?5- What characterized the works of Eric Johansson photographs?6- Is Eric Johansson Founder of one of the general surrealist trends and photographic Special؟7- What are the features and characteristics of surrealist photography?

Photograph design is an original part of it, and the creativity of the photographer depends on its ability to use the elements of the image to produce creative design, using these elements innovatively. The contemporary technological development has helped to create the image faster, and less time, not only when photography exists, Grid), but gave some cameras the possibility of cutting and modifying and even work on the image on the inside of the camera, and with the potential of broadcasting from the camera on the computer or mobile via Wi-Fi has become both photography and the transfer of the image quickly and directly from the location of photography to anywhere in the world does not constitute No difficulty like the previous.Technology is the key to photography, and its integration with the eye of creative photography produces original and innovative images, and with the development of digital processing programs for photographs such as Photoshop has been integrated some of the characteristics that enabled the rapid access to a distinctive photographic balance, but a number of factors helping to produce more innovative images accurately And faster.The size of the sensors varies depending on the manufacturer of the devices producing the digital image, whether it is a camera or a mobile phone.The problem of research is the definition of innovative strategy to produce and improve the level of innovation in the balance of the image through the integration of both technology and photography using the program Photoshop to provide a picture visually integrated and the extent of the possibility of applying the rules of configuration on all sizes of sensors and the main question is whether the application of theories and practices of composition with different The size of the sensations of a rectangle of a square as well as the difference of proportions of rectangles from one factory to another.In the research results, we find that technology is an integral part of creating a photographic image in our digital work. It encourages innovation by studying design, especially balancing, using the golden ratio, the primary element in any base of the composition, which helps to solve the problem of creativity and access to a distinct original production. And we are convinced that creativity is not affected by the different sizes of sensors, which ultimately leads to the difference of the final image.

This study was performed to elucidate the effects of linoleic acid (LA), oleic acid (OA) and their combination (LA + OA) on cell proliferation, apoptosis, necrosis, and the lipid metabolism related gene expression in bovine satellite cells (BSCs), isolated from bovine muscles. Cell viability was significantly increased with the OA and LA treatment. Furthermore, LA + OA enhanced cell proliferation in a dose-dependent manner (10 to 100 µM), whereas it lowered at 250 µM. In addition, a cell-cycle analysis showed that 100 µM of LA and OA markedly decreased the G0/G1 phase proportion (62.58% and 61.33%, respectively), compared to controls (68.02%), whereas the S-phase cells’ proportion was increased. The ratio of G2/M phase cells was not significantly different among the groups. Moreover, analyses with AO/EtBr staining showed that no apoptosis occurred. Necrosis were determined by flow cytometry using Annexin V-FITC/PI staining which revealed no early apoptosis in the cells pretreated with LA or OA, but occurred in the LA + OA group. We also analyzed the mRNA expression of lipid metabolizing genes such as peroxisome proliferator receptor alfa (PPARα), peroxisome proliferator receptor gamma (PPARγ), acyl-CoA oxidase (ACOX), lipoprotein lipase (LPL), carnitine palmitoyl transferase (CPT-1), and fatty-acid binding protein4 (FABP4), which were upregulated in LA or OA treated cells compared to the control group. In essence, LA and OA alone promote the cell proliferation without any apoptosis and necrosis, which might upregulate the lipid metabolism related gene expressions, and increase fatty-acid oxidation in the BSCs’ lipid metabolism.

Kaempferol, a phytochemical found in many edible plants, is known to alleviate diseases such as cancer, allergy, and inflammation. The objective of this study was to investigate whether kaempferol could reduce omega-6 and ovalbumin-mediated allergic reactions at lung and trachea in BALB/c mice. Mice were allocated into five groups: 1) control group (CON); 2) positive control group with orally administration of omega-6 (POS); 3) bovine serum albumin (BSA) sensitization group (with BSA injection and ovalbumin inhalation); 4) BSA+K10 group: BSA injection, 10 μg/g of kaempferol administration and ovalbumin inhalation; and 5) BSA+K20 group: BSA injection, 20 μg/g of kaempferol administration and ovalbumin inhalation. Results revealed that serum histamine level was the highest (p

This study was conducted to determine the effects of garlic on egg per gram (EPG) count of feces for gastrointestinal parasites, live weight, and hematological parameters in Black Bengal goat. A total of 18 dry does of 18-22 months of age were divided into 3 groups as T0, T1 and T2; where, each group comprised of 6 goats. The goats of T0 were feed with normal feeds, whereas the goats of T1 and T2 were fed with normal feeds plus 25 mL and 50 mL of 10% water solution of garlic twice per day, respectively for 60 days. The EPG count was performed by McMaster counting chamber, and live weight was measured by digital electric balance. EPG count for gastrointestinal parasites was found significantly lower in the treatment groups as compared to T0. Weight gain was recorded significantly higher in the treatment groups. The hematological parameters like total leucocyte count (TLC), erythrocyte sedimentation rate (ESR), packed cell volume (PCV), hemoglobin (Hb) and total erythrocyte count (TEC) showed significant changes in the treatment groups. The study suggests that 10% water solution of garlic is a useful supplementation to decrease EPG count, body weight gain; thus, the 10% aqueous garlic solution can improve the general health condition of goat. [J Adv Vet Anim Res 2015; 2(3.000): 326-331]