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33 results on '"Albarrán, Cristina"'

1. Referral of Patients to Dermatology and Teledermatology Consultations in Spain. DIADERM Study

Author

Buendía, Agustín, Fernández-Crehuet, Pablo, Husein-ElAhmed, Husein, Vega, Jesús, Viera, Agustín, Manuel Carrascosa, José, Ferrán, Marta, Gómez, Enrique, Ascanio, Lucia, García Doval, Ignacio, Arias, Salvador, Gilaberte, Yolanda, Sánchez, Juan A., Serrano, Amalia, Castillo, Rosa, Fernandez, Ramón, Armario, José, Lluc Cantalejo, Carolina, Albarrán, Cristina, Cruz Martín, María, Martín, Juan Antonio, Barabash, Román, Pérez, Lara, Salamanca, Manuel, Hernández, Carlos, Millán, José Francisco, Ruiz, Inmaculada, Armesto, Susana, González, Marta, Beteta, Valia, Cuadrado de Valles, Concepción, Cristóbal, Pilar, Roth, María Magdalena, Garcias, Juan, Fernandez de Misa, Ricardo, García, Estela, Rivero, María del Pino, Suárez, José, Farthmann, Birgit, Álvarez, Alba, García, Irene, Morales, Caridad Elena, Zemba, María Cristina, Repiso, Trinidad, Sastre, Carmen, Ubals, María, Fernández, Alejandro, González, Urbà, Grimalt, Ramón, Gómez, Sara, López, Ingrid, Gemigniani, Franco Antonio, Izquierdo, María José, Alfageme, Fernando, Barrientos, Nuria, Pericet, Laura María, Vidal, Santiago, Camarero, Celia, Lázaro, Pablo, García, Cristina, de Pablo, María Pilar, Herranz, Pedro, del Olmo, Natalia, Castellanos, María, Jiménez, Natalia, Aboín, Sonsoles, Aldanondo, Isabel, Juanes, Adriana, Arranz, Dulce María, González, Olga, Casas, Luis, Vázquez, Juan José, Peña, Carmen, Cubero, José Luis, Feal, Carlos, Mayo, María Eugenia, Iglesias, Nicolás, Rojo, Rafael, Aniz, Elfidia, Kindem, Sabrina, Barrado, Nerea, Tirado, Marisa, Quecedo, Ester, Hernández, Isabel, Sahuquillo, Antonio, Bella, Rebeca, García, Ramón, Calle, Anaid, Messeguer, Francesc, Alfaro, Alberto, Casanova, Luisa, Aspe, Libe, Moreno, María Pilar, Trébol, Izaskun, Serrano, Gonzalo, Alcalde, Víctor Manuel, García, Patricia, Coscojuela, Carmen, González-López, G., Descalzo-Gallego, M.Á., Arias-Santiago, S., Molina-Leyva, A., Gilaberte, Y., Fernández-Crehuet, P., Husein-El Ahmed, H., Viera-Ramírez, A., Fernández-Peñas, P., Taberner, R., García-Doval, I., and Buendía-Eisman, A.

Published
2019
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2. Derivación de pacientes en consulta de dermatología y de teledermatología en España. Estudio DIADERM

Author

Buendía, Agustín, Fernández-Crehuet, Pablo, Husein-ElAhmed, Husein, Vega, Jesús, Viera, Agustín, Manuel Carrascosa, José, Ferrán, Marta, Gómez, Enrique, Ascanio, Lucia, García Doval, Ignacio, Arias, Salvador, Gilaberte, Yolanda, Sánchez, Juan A., Serrano, Amalia, Castillo, Rosa, Fernandez, Ramón, Armario, José, Lluc Cantalejo, Carolina, Albarrán, Cristina, Cruz Martín, María, Martín, Juan Antonio, Barabash, Román, Pérez, Lara, Salamanca, Manuel, Hernández, Carlos, Millán, José Francisco, Ruiz, Inmaculada, Armesto, Susana, González, Marta, Beteta, Valia, Cuadrado de Valles, Concepción, Cristóbal, Pilar, Roth, María Magdalena, Garcias, Juan, Fernandez de Misa, Ricardo, García, Estela, Rivero, María del Pino, Suárez, José, Farthmann, Birgit, Álvarez, Alba, García, Irene, Morales, Caridad Elena, Zemba, María Cristina, Repiso, Trinidad, Sastre, Carmen, Ubals, María, Fernández, Alejandro, González, Urbà, Grimalt, Ramón, Gómez, Sara, López, Ingrid, Gemigniani, Franco Antonio, Izquierdo, María José, Alfageme, Fernando, Barrientos, Nuria, Pericet, Laura María, Vidal, Santiago, Camarero, Celia, Lázaro, Pablo, García, Cristina, de Pablo, María Pilar, Herranz, Pedro, del Olmo, Natalia, Castellanos, María, Jiménez, Natalia, Aboín, Sonsoles, Aldanondo, Isabel, Juanes, Adriana, Arranz, Dulce María, González, Olga, Casas, Luis, Vázquez, Juan José, Peña, Carmen, Cubero, José Luis, Feal, Carlos, Mayo, María Eugenia, Iglesias, Nicolás, Rojo, Rafael, Aniz, Elfidia, Kindem, Sabrina, Barrado, Nerea, Tirado, Marisa, Quecedo, Ester, Hernández, Isabel, Sahuquillo, Antonio, Bella, Rebeca, García, Ramón, Calle, Anaid, Messeguer, Francesc, Alfaro, Alberto, Casanova, Luisa, Aspe, Libe, Moreno, María Pilar, Trébol, Izaskun, Serrano, Gonzalo, Alcalde, Víctor Manuel, García, Patricia, Coscojuela, Carmen, González-López, G., Descalzo-Gallego, M.Á., Arias-Santiago, S., Molina-Leyva, A., Gilaberte, Y., Fernández-Crehuet, P., Husein-El Ahmed, H., Viera-Ramírez, A., Fernández-Peñas, P., Taberner, R., García-Doval, I., and Buendía-Eisman, A.

Published
2019
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3. Forensic analysis of dog (Canis lupus familiaris) mitochondrial DNA sequences: An inter-laboratory study of the GEP-ISFG working group

Author

van Asch, Barbara, Albarran, Cristina, Alonso, Antonio, Angulo, Ramón, Alves, Cíntia, Betancor, Eva, Catanesi, Cecilia I., Corach, Daniel, Crespillo, Manuel, Doutremepuich, Christian, Estonba, Andone, Fernandes, Ana Teresa, Fernandez, Eugenia, Garcia, Ana Maria, Garcia, Miguel Angel, Gilardi, Patricia, Gonçalves, Rita, Hernández, Alexis, Lima, Gabriela, Nascimento, Eugênio, de Pancorbo, Marian M., Parra, David, Pinheiro, Maria de Fátima, Prat, Elena, Puente, Jorge, Ramírez, José Luis, Rendo, Fernando, Rey, Isabel, Di Rocco, Florencia, Rodríguez, Anayanci, Sala, Andrea, Salla, Joel, Sanchez, Juan J., Solá, Dácil, Silva, Sandra, Pestano Brito, José Juan, and Amorim, António

Published
2009
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5. Referral of Patients to Dermatology and Teledermatology Consultations in Spain. DIADERM Study

Author

González-López, G., primary, Descalzo-Gallego, M.Á., additional, Arias-Santiago, S., additional, Molina-Leyva, A., additional, Gilaberte, Y., additional, Fernández-Crehuet, P., additional, Husein-El Ahmed, H., additional, Viera-Ramírez, A., additional, Fernández-Peñas, P., additional, Taberner, R., additional, García-Doval, I., additional, Buendía-Eisman, A., additional, Buendía, Agustín, additional, Fernández-Crehuet, Pablo, additional, Husein-ElAhmed, Husein, additional, Vega, Jesús, additional, Viera, Agustín, additional, Manuel Carrascosa, José, additional, Ferrán, Marta, additional, Gómez, Enrique, additional, Ascanio, Lucia, additional, García Doval, Ignacio, additional, Arias, Salvador, additional, Gilaberte, Yolanda, additional, Sánchez, Juan A., additional, Serrano, Amalia, additional, Castillo, Rosa, additional, Fernandez, Ramón, additional, Armario, José, additional, Lluc Cantalejo, Carolina, additional, Albarrán, Cristina, additional, Cruz Martín, María, additional, Martín, Juan Antonio, additional, Barabash, Román, additional, Pérez, Lara, additional, Salamanca, Manuel, additional, Hernández, Carlos, additional, Millán, José Francisco, additional, Ruiz, Inmaculada, additional, Armesto, Susana, additional, González, Marta, additional, Beteta, Valia, additional, Cuadrado de Valles, Concepción, additional, Cristóbal, Pilar, additional, Roth, María Magdalena, additional, Garcias, Juan, additional, Fernandez de Misa, Ricardo, additional, García, Estela, additional, Rivero, María del Pino, additional, Suárez, José, additional, Farthmann, Birgit, additional, Álvarez, Alba, additional, García, Irene, additional, Morales, Caridad Elena, additional, Zemba, María Cristina, additional, Repiso, Trinidad, additional, Sastre, Carmen, additional, Ubals, María, additional, Fernández, Alejandro, additional, González, Urbà, additional, Grimalt, Ramón, additional, Gómez, Sara, additional, López, Ingrid, additional, Gemigniani, Franco Antonio, additional, Izquierdo, María José, additional, Alfageme, Fernando, additional, Barrientos, Nuria, additional, Pericet, Laura María, additional, Vidal, Santiago, additional, Camarero, Celia, additional, Lázaro, Pablo, additional, García, Cristina, additional, de Pablo, María Pilar, additional, Herranz, Pedro, additional, del Olmo, Natalia, additional, Castellanos, María, additional, Jiménez, Natalia, additional, Aboín, Sonsoles, additional, Aldanondo, Isabel, additional, Juanes, Adriana, additional, Arranz, Dulce María, additional, González, Olga, additional, Casas, Luis, additional, Vázquez, Juan José, additional, Peña, Carmen, additional, Cubero, José Luis, additional, Feal, Carlos, additional, Mayo, María Eugenia, additional, Iglesias, Nicolás, additional, Rojo, Rafael, additional, Aniz, Elfidia, additional, Kindem, Sabrina, additional, Barrado, Nerea, additional, Tirado, Marisa, additional, Quecedo, Ester, additional, Hernández, Isabel, additional, Sahuquillo, Antonio, additional, Bella, Rebeca, additional, García, Ramón, additional, Calle, Anaid, additional, Messeguer, Francesc, additional, Alfaro, Alberto, additional, Casanova, Luisa, additional, Aspe, Libe, additional, Moreno, María Pilar, additional, Trébol, Izaskun, additional, Serrano, Gonzalo, additional, Alcalde, Víctor Manuel, additional, García, Patricia, additional, and Coscojuela, Carmen, additional

Published
2019
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6. Derivación de pacientes en consulta de dermatología y de teledermatología en España. Estudio DIADERM

Author

González-López, G., primary, Descalzo-Gallego, M.Á., additional, Arias-Santiago, S., additional, Molina-Leyva, A., additional, Gilaberte, Y., additional, Fernández-Crehuet, P., additional, Husein-El Ahmed, H., additional, Viera-Ramírez, A., additional, Fernández-Peñas, P., additional, Taberner, R., additional, García-Doval, I., additional, Buendía-Eisman, A., additional, Buendía, Agustín, additional, Fernández-Crehuet, Pablo, additional, Husein-ElAhmed, Husein, additional, Vega, Jesús, additional, Viera, Agustín, additional, Manuel Carrascosa, José, additional, Ferrán, Marta, additional, Gómez, Enrique, additional, Ascanio, Lucia, additional, García Doval, Ignacio, additional, Arias, Salvador, additional, Gilaberte, Yolanda, additional, Sánchez, Juan A., additional, Serrano, Amalia, additional, Castillo, Rosa, additional, Fernandez, Ramón, additional, Armario, José, additional, Lluc Cantalejo, Carolina, additional, Albarrán, Cristina, additional, Cruz Martín, María, additional, Martín, Juan Antonio, additional, Barabash, Román, additional, Pérez, Lara, additional, Salamanca, Manuel, additional, Hernández, Carlos, additional, Millán, José Francisco, additional, Ruiz, Inmaculada, additional, Armesto, Susana, additional, González, Marta, additional, Beteta, Valia, additional, Cuadrado de Valles, Concepción, additional, Cristóbal, Pilar, additional, Roth, María Magdalena, additional, Garcias, Juan, additional, Fernandez de Misa, Ricardo, additional, García, Estela, additional, Rivero, María del Pino, additional, Suárez, José, additional, Farthmann, Birgit, additional, Álvarez, Alba, additional, García, Irene, additional, Morales, Caridad Elena, additional, Zemba, María Cristina, additional, Repiso, Trinidad, additional, Sastre, Carmen, additional, Ubals, María, additional, Fernández, Alejandro, additional, González, Urbà, additional, Grimalt, Ramón, additional, Gómez, Sara, additional, López, Ingrid, additional, Gemigniani, Franco Antonio, additional, Izquierdo, María José, additional, Alfageme, Fernando, additional, Barrientos, Nuria, additional, Pericet, Laura María, additional, Vidal, Santiago, additional, Camarero, Celia, additional, Lázaro, Pablo, additional, García, Cristina, additional, de Pablo, María Pilar, additional, Herranz, Pedro, additional, del Olmo, Natalia, additional, Castellanos, María, additional, Jiménez, Natalia, additional, Aboín, Sonsoles, additional, Aldanondo, Isabel, additional, Juanes, Adriana, additional, Arranz, Dulce María, additional, González, Olga, additional, Casas, Luis, additional, Vázquez, Juan José, additional, Peña, Carmen, additional, Cubero, José Luis, additional, Feal, Carlos, additional, Mayo, María Eugenia, additional, Iglesias, Nicolás, additional, Rojo, Rafael, additional, Aniz, Elfidia, additional, Kindem, Sabrina, additional, Barrado, Nerea, additional, Tirado, Marisa, additional, Quecedo, Ester, additional, Hernández, Isabel, additional, Sahuquillo, Antonio, additional, Bella, Rebeca, additional, García, Ramón, additional, Calle, Anaid, additional, Messeguer, Francesc, additional, Alfaro, Alberto, additional, Casanova, Luisa, additional, Aspe, Libe, additional, Moreno, María Pilar, additional, Trébol, Izaskun, additional, Serrano, Gonzalo, additional, Alcalde, Víctor Manuel, additional, García, Patricia, additional, and Coscojuela, Carmen, additional

Published
2019
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8. The Specificity in the Interaction between Cytochrome f and Plastocyanin from the Cyanobacterium Nostoc sp. PCC 7119 Is Mainly Determined by the Copper Protein

Author

Albarrán, Cristina, Navarro, José A., Rosa, Miguel A. de la, Hervás, Manuel, Albarrán, Cristina, Navarro, José A., Rosa, Miguel A. de la, and Hervás, Manuel

Abstract

The plastocyanin-cytochrome f complex from Nostoc exhibits relevant structural differences when compared with the homologous complexes from other cyanobacteria and plants, with electrostatic and hydrophobic interactions being differently involved in each case. Here, five negatively charged residues of a recombinant form of cytochrome f from Nostoc have been replaced with either neutral or positively charged residues, and the effects of mutations on the kinetics of electron transfer to wild-type and mutant forms of plastocyanin have been measured by laser flash absorption spectroscopy. Cytochrome f mutants with some negative charges replaced with neutral residues exhibit an apparent electron transfer rate constant with wild-type plastocyanin similar to or slightly higher than that of the wild-type species, whereas the mutants with negative charges replaced with positive residues exhibit a significantly lower reactivity. Taken together, these results indicate that the effects of neutralizing residues at the electrostatically charged patch of cytochrome f are smaller than those previously observed for mutants of plastocyanin, thus suggesting that it is the copper protein which determines the specificity of the electrostatic interaction with the heme protein. Moreover, cross reactions between mutants of both proteins reveal the presence of some short-range specific electrostatic interactions. Our findings also make evident the fact that in Nostoc the main contribution to the electrostatic nature of the complex is provided by the small domain of cytochrome

Published
2007

9. Laser flash-induced kinetic analysis of cytochrome f oxidation by wild-type and mutant plastocyanin from the cyanobacterium Nostoc sp. PCC 7119

Author

Albarrán, Cristina, Navarro, José A., Molina-Heredia, Fernando P., Murdoch, Piedad del S., Rosa, Miguel A. de la, Hervás, Manuel, Albarrán, Cristina, Navarro, José A., Molina-Heredia, Fernando P., Murdoch, Piedad del S., Rosa, Miguel A. de la, and Hervás, Manuel

Abstract

Oxidation of the soluble, truncated form of cytochrome f by wild-type and mutant species of plastocyanin has been analyzed by laser flash absorption spectroscopy in the cyanobacterium Nostoc (formerly, Anabaena) sp. PCC 7119. At low ionic strengths, the apparent electron transfer rate constant of cytochrome f oxidation by wild-type plastocyanin is 1.34 × 10^4 s-1, a value much larger than those determined for the same proteins from other organisms. Upon site-directed mutagenesis of specific residues at the plastocyanin interaction area, the rate constant decreases in all cases yet to varying extents. The only exception is the D54K variant, which exhibits a higher reactivity toward cytochrome f. In most cases, the reaction rate constant decreases monotonically with an increase in ionic strength. The observed changes in the reaction mechanism and rate constants are in agreement with the location of the mutated residues at the interface area, as well as with the peculiar orientation of the two partners within the Nostoc plastocyanin−cytochrome f transient complex, whose NMR structure has been determined recently. Furthermore, the experimental data herein reported match well the kinetic behavior exhibited by the same set of plastocyanin mutants when acting as donors of electrons to photosystem I [Molina-Heredia, F. P., et al. (2001) J. Biol. Chem. 276, 601−605], thus indicating that the copper protein uses the same surface areas -one hydrophobic and the other electrostatic- to interact with both cytochrome f and photosystem I.

Published
2005

12. Evaluating the forensic informativeness of mtDNA haplogroup H sub-typing on a Eurasian scale

Author

Pereira, Luísa, primary, Richards, Martin, additional, Goios, Ana, additional, Alonso, Antonio, additional, Albarrán, Cristina, additional, Garcia, Oscar, additional, Behar, Doron M., additional, Gölge, Mukaddes, additional, Hatina, Jiři, additional, Al-Gazali, Lihadh, additional, Bradley, Daniel G., additional, Macaulay, Vincent, additional, and Amorim, António, additional

Published
2006
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13. Distribution of Y-chromosomal haplotypes in the Basque Country autochthonous population using a 17-locus multiplex PCR assay

Author

García, Oscar, primary, Yurrebaso, Iñaki, additional, Uriarte, Ion, additional, Pérez, Juan Antonio, additional, Peñas, Raúl, additional, Alonso, Santos, additional, de la Rua, Concepción, additional, Izagirre, Neskuts, additional, Flores, Carlos, additional, Martín, Pablo, additional, Albarrán, Cristina, additional, and Alonso, Antonio, additional

Published
2006
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18. Mitochondrial DNA error prophylaxis: assessing the causes of errors in the GEP’02–03 proficiency testing trial

Author

Salas, Antonio, primary, Prieto, Lourdes, additional, Montesino, Marta, additional, Albarrán, Cristina, additional, Arroyo, Eduardo, additional, Paredes-Herrera, Miguel R., additional, Di Lonardo, Ana Ma, additional, Doutremepuich, Christian, additional, Fernández-Fernández, Isabel, additional, de la Vega, Alberto González, additional, Alves, Cı́ntia, additional, López, Carlos M., additional, López-Soto, Manolo, additional, Lorente, José A., additional, Picornell, Antònia, additional, Espinheira, Rosa M., additional, Hernández, Alexis, additional, Palacio, Ana Ma, additional, Espinoza, Marta, additional, Yunis, Juan J., additional, Pérez-Lezaun, Anna, additional, Pestano, José J., additional, Carril, Juan Carlos, additional, Corach, Daniel, additional, Vide, Ma Conceiçao, additional, Álvarez-Iglesias, V., additional, Pinheiro, M.F., additional, Whittle, Martı́n R., additional, Brehm, Antonio, additional, and Gómez, Josefina, additional

Published
2005
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19. High-resolution mtDNA evidence for the late-glacial resettlement of Europe from an Iberian refugium

Author

Pereira, Luísa, primary, Richards, Martin, additional, Goios, Ana, additional, Alonso, Antonio, additional, Albarrán, Cristina, additional, Garcia, Oscar, additional, Behar, Doron M., additional, Gölge, Mukaddes, additional, Hatina, Jiři, additional, Al-Gazali, Lihadh, additional, Bradley, Daniel G., additional, Macaulay, Vincent, additional, and Amorim, António, additional

Published
2005
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20. A Basque Country autochthonous population study of 11 Y-chromosome STR loci

Author

Garcı́a, Oscar, primary, Martı́n, Pablo, additional, Gusmão, Leonor, additional, Albarrán, Cristina, additional, Alonso, Santos, additional, de la Rua, Concepción, additional, Flores, Carlos, additional, Izagirre, Neskuts, additional, Peñas, Raúl, additional, Antonio Pérez, Juan, additional, Uriarte, Ion, additional, Yurrebaso, Iñaki, additional, and Alonso, Antonio, additional

Published
2004
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21. A Spanish population study of 17 Y-chromosome STR loci

Author

Martı́n, Pablo, primary, Garcı́a-Hirschfeld, Julia, additional, Garcı́a, Oscar, additional, Gusmão, Leonor, additional, Garcı́a, Pilar, additional, Albarrán, Cristina, additional, Sancho, Manuel, additional, and Alonso, Antonio, additional

Published
2004
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23. The 2000–2001 GEP–ISFG Collaborative Exercise on mtDNA: assessing the cause of unsuccessful mtDNA PCR amplification of hair shaft samples

Author

Prieto, Lourdes, primary, Montesino, Marta, additional, Salas, Antonio, additional, Alonso, Antonio, additional, Albarrán, Cristina, additional, Álvarez, Sara, additional, Crespillo, Manuel, additional, Di Lonardo, Ana Ma, additional, Doutremepuich, Christian, additional, Fernández-Fernández, Isabel, additional, de la Vega, Alberto González, additional, Gusmão, Leonor, additional, López, Carlos M, additional, López-Soto, Manolo, additional, Lorente, José A, additional, Malaghini, Marcelo, additional, Martı́nez, Carlos A, additional, Modesti, Nidia M, additional, Palacio, Ana Ma, additional, Paredes, Manuel, additional, Pena, Sergio D.J, additional, Pérez-Lezaun, Anna, additional, Pestano, José J, additional, Puente, Jorge, additional, Sala, Andrea, additional, Vide, MaConceiçao, additional, Whittle, Martı́n R, additional, Yunis, Juan J, additional, and Gómez, Josefina, additional

Published
2003
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24. Multiplex–PCR of short amplicons for mtDNA sequencing from ancient DNA

Author

Alonso, Antonio, primary, Albarrán, Cristina, additional, Martı́n, Pablo, additional, Garcı́a, Pilar, additional, Garcı́a, Oscar, additional, de la Rúa, Concepción, additional, Alzualde, Ainhoa, additional, Fernández de Simón, Lourdes, additional, Sancho, Manuel, additional, and Fernández Piqueras, José, additional

Published
2003
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25. Results of the 1999–2000 collaborative exercise and proficiency testing program on mitochondrial DNA of the GEP-ISFG: an inter-laboratory study of the observed variability in the heteroplasmy level of hair from the same donor

Author

Alonso, Antonio, primary, Salas, Antonio, additional, Albarrán, Cristina, additional, Arroyo, Eduardo, additional, Castro, Azucena, additional, Crespillo, Manuel, additional, di Lonardo, Ana Marı́a, additional, Lareu, Marı́a Victoria, additional, Cubrı́a, Carlos López, additional, Soto, Manuel López, additional, Lorente, José A, additional, Semper, Marta Montesino, additional, Palacio, Ana, additional, Paredes, Manuel, additional, Pereira, Luisa, additional, Lezaun, Anna Pérez, additional, Brito, José Pestano, additional, Sala, Andrea, additional, Vide, Marı́a Conceiçao, additional, Whittle, Martı́n, additional, Yunis, Juan J, additional, and Gómez, Josefina, additional

Published
2002
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27. A Hinfl polymorphism in the 5'flanking region of the human VNTR locus DIS80.

Author

Alonso, Antonio, Martin, Pablo, Albarrán, Cristina, and Sancho, Manuel

Abstract

A restriction fragment length polymorphism (RFLP) characterized by the presence ( HinfI+) or absence ( HinfI-) of a HitinfI site has been found in the 5′ flanking region of the VNTR locus D 1 S80. RFLP-allele frequencies were determined from 82 unrelated individuals: HinfI+ = 0.49, HinfI- = 0.51. The RFLP/VNTR haplotype frequencies show an absolute association between the HinfI+ allele and the VNTR allele of 18 repeat units and an extreme association between the HinfI- allele and the VNTR allele of 24 repeat units. The remaining VNTR alleles associate more randomly with the 2 flanking HinfI alleles. [ABSTRACT FROM AUTHOR]

Published
1995
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28. Challenges of DNA Profiling in Mass Disaster Investigations.

Author

Alonso, Antonio, Martín, Pablo, Albarrán, Cristina, Garcí, Pilar, De Simón, Lourdes Fernández, Iturralde, Maria Jesús, Fernández-Rodríguez, Amparo, Atienza, Inmaculada, Capilla, Javier, García-Hirschfeld, Julia, Martínez, Pilar, Vallejo, Gloria, García, Oscar, García, Emilio, Real, Pilar, Álvarez, David, León, Antonio, and Sancho, Manuel

Subjects
*DNA fingerprinting, *FORENSIC genetics techniques, *IDENTIFICATION, *DISASTERS, *DISASTER victims
Abstract

In cases of mass disaster, there is often a need for managing, analyzing, and comparing large numbers of biological samples and DNA profiles. This requires the use of laboratory information management systems for large-scale sample logging and tracking, coupled with bioinformatic tools for DNA database searching according to different matching algorithms, and for the evaluation of the significance of each match by likelihood ratio calculations. There are many different interrelated factors and circumstances involved in each specific mass disaster scenario that may challenge the final DNA identification goal, such as the number of victims, the mechanisms of body destruction, the extent of body fragmentation, the rate of DNA degradation, the body accessibility for sample collection, or the type of DNA reference samples availability. In this paper, we examine the different steps of the DNA identification analysis (DNA sampling, DNA analysis and technology, DNA database searching, and concordance and kinship analysis) reviewing the "lessons learned" and the scientific progress made in some mass disaster cases described in the scientific literature. We will put special emphasis on the valuable scientific feedback that genetic forensic community has received from the collaborative efforts of several public and private USA forensic laboratories in assisting with the more critical areas of the World Trade Center (WTC) mass fatality of September 11, 2001. The main challenges in identifying the victims of the recent South Asian Tsunami disaster, which has produced the steepest death count rise in history, will also be considered. We also present data from two recent mass fatality cases that involved Spanish victims: the Madrid terrorist attack of March 11, 2004, and the Yakolev-42 aircraft accident in Trabzon, Turkey, of May 26, 2003. [ABSTRACT FROM AUTHOR]

Published
2005

29. Real-time PCR designs to estimate nuclear and mitochondrial DNA copy number in forensic and ancient DNA studies

Author

Alonso, Antonio, Martín, Pablo, Albarrán, Cristina, García, Pilar, García, Oscar, de Simón, Lourdes Fernández, García-Hirschfeld, Julia, Sancho, Manuel, de la Rúa, Concepción, and Fernández-Piqueras, Jose

Subjects
*DNA polymerases, *POLYMERASE chain reaction, *MITOCHONDRIAL DNA, *FORENSIC sciences
Abstract

We explore different designs to estimate both nuclear and mitochondrial human DNA (mtDNA) content based on the detection of the 5′ nuclease activity of the Taq DNA polymerase using fluorogenic probes and a real-time quantitative PCR detection system. Human mtDNA quantification was accomplished by monitoring the real-time progress of the PCR-amplification of two different fragment sizes (113 and 287 bp) within the hypervariable region I (HV1) of the mtDNA control region, using two fluorogenic probes to specifically determine the mtDNA copy of each fragment size category. This mtDNA real-time PCR design has been used to assess the mtDNA preservation (copy number and degradation state) of DNA samples retrieved from 500 to 1500 years old human remains that showed low copy number and highly degraded mtDNA. The quantification of nuclear DNA was achieved by real-time PCR of a segment of the X–Y homologous amelogenin (AMG) gene that allowed the simultaneous estimation of a Y-specific fragment (AMGY: 112 bp) and a X-specific fragment (AMGX: 106 bp) making possible not only haploid or diploid DNA quantitation but also sex determination. The AMG real-time PCR design has been used to quantify a set of 57 DNA samples from 4–5 years old forensic bone remains with improved sensitivity compared with the slot–blot hybridization method. The potential utility of this technology to improve the quality of some PCR-based forensic and ancient DNA studies (microsatellite typing and mtDNA sequencing) is discussed. [Copyright &y& Elsevier]

Published
2004
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30. A Basque Country autochthonous population study of 11 Y-chromosome STR loci

Author

García, Oscar, Martín, Pablo, Gusmão, Leonor, Albarrán, Cristina, Alonso, Santos, de la Rua, Concepción, Flores, Carlos, Izagirre, Neskuts, Peñas, Raúl, Antonio Pérez, Juan, Uriarte, Ion, Yurrebaso, Iñaki, and Alonso, Antonio

Subjects
*Y chromosome, *SEX chromosomes, *POPULATION, *CELL nuclei
Abstract

Haplotype, allele frequencies and population data of 11 Y-chromosome STR loci DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438 and DYS439 were determined from a sample of 168 unrelated autochthonous male individuals from the Basque Country. The eight surnames and birth places of the grandparents of all analyzed individuals were of Basque origin. A total of 89 haplotypes were identified by the 11 Y-STR loci. The haplotype diversity (97.49%) and discrimination capacity (52.98%) were calculated. Comparisons were made with previously published haplotype data on other Iberian population samples and significant differences were found. [Copyright &y& Elsevier]

Published
2004
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31. A Spanish population study of 17 Y-chromosome STR loci

Author

Martín, Pablo, García-Hirschfeld, Julia, García, Oscar, Gusmão, Leonor, García, Pilar, Albarrán, Cristina, Sancho, Manuel, and Alonso, Antonio

Subjects
*Y chromosome, *POPULATION genetics, *LOCUS (Genetics)
Abstract

Haplotype, allele frequencies and population data of 17 Y-chromosome STR loci DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460 (GATA A7.1), DYS461 (GATA A7.2), GATA A10, GATA C4 and GATA H4 were determined from a sample of 148 unrelated male individuals from Spain. A total of 144 haplotypes were identified by the 17 Y-STR markers, of which 141 were unique, two were found in two individuals and one was found in three individuals. The haplotype diversity (99.95%) and discrimination capacity (97.30%) were calculated.Comparisons were made with previously published haplotype data on other Iberian population samples and no significant differences were found. [Copyright &y& Elsevier]

Published
2004
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32. Specific quantification of human genomes from low copy number DNA samples in forensic and ancient DNA studies.

Author

Alonso A, Martin P, Albarrán C, García P, Primorac D, García O, Fernández de Simón L, García-Hirschfeld J, Sancho M, and Fernández-Piqueras J

Subjects
Amelogenin, Complementarity Determining Regions, DNA, Mitochondrial genetics, Dental Enamel Proteins genetics, Humans, Polymerase Chain Reaction methods, Sequence Analysis, DNA, Sex Determination Analysis methods, Tandem Repeat Sequences, DNA Fingerprinting methods, Forensic Anthropology methods
Abstract

We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear and mitochondrial DNA regions. Special emphasis has been put on the applicability of recently described different real-time PCR designs targeting different fragments of the HV1 mtDNA control region, and a segment of the X-Y homologous amelogenin gene. The importance of these quantitative assays is to ensure the consistency of low copy number DNA typing (STR profiling and mtDNA sequencing).

Published
2003

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