1. Genome-wide screening of mRNA expression in leprosy patients.
- Author
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Andrea Faria Fernandes Belone, Patrícia Sammarco Rosa, Ana Paula Favaro Trombone, Luciana Raquel Vincenzi Fachin, Cássio César Guidella, Somei eUra, Jaison Antonio Barreto, Mabel Gigliola Pinilla, Alex Fiorini de Carvalho, Dirce Maria Carraro, Fernando Augusto Soares, and Cleverson Teixeira Soares
- Subjects
Leprosy ,Microarray ,mRNA ,Signaling Pathways ,immunohistochemistry (IHC) ,Genetics ,QH426-470 - Abstract
Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female). The evaluation identified 1,580 differentially expressed mRNAs [Fold Change (FC)≥2.0, p
- Published
- 2015
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