1. ABCG transporters export cutin precursors for the formation of the plant cuticle
- Author
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Markus Geisler, Ignacio Martinez San Segundo, Jie Liu, Asaph Aharoni, Carolina Elejalde-Palmett, Mads Hartvig Clausen, Antonio Mucciolo, Lukas Schreiber, Christiane Nawrath, Viktoria Zeisler-Diehl, Damien De Bellis, Imène Garroum, Bénédicte Bakan, Laurence Charrier, Aurore Guerault, University of Lausanne (UNIL), Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark, University of Fribourg, University of Bonn, Weizmann Institute of Science [Rehovot, Israël], Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Swiss National Science Foundation, Switzerland (grants 31003A-125009,31003A-146276, and 31003A-170127 to C.N. and 31003A-165877 to M.G.).M.H.C. The Novo Nordisk Foundation, Denmark, for research sup-port (grant no. 444-11319) and to DTU, the Deutsche Forschungsgemeinschaft,Germany.
- Subjects
0301 basic medicine ,Cuticle ,Arabidopsis ,Nicotiana benthamiana ,ATP Binding Cassette Transporter, Subfamily G ,ATP-binding cassette transporter ,Cutin ,tomato ,Biology ,plasma membrane ,General Biochemistry, Genetics and Molecular Biology ,Plant Epidermis ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,Membrane Lipids ,03 medical and health sciences ,0302 clinical medicine ,Solanum lycopersicum ,Gene Expression Regulation, Plant ,Tobacco ,diffusion barrier ,Plant Proteins ,acyl lipid ,wax ,Arabidopsis Proteins ,cutin ,fungi ,food and beverages ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Protoplast ,biology.organism_classification ,Apoplast ,Plant Leaves ,[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding ,030104 developmental biology ,Biochemistry ,Plant cuticle ,transport ,cuticle ,ABC transporter ,General Agricultural and Biological Sciences ,030217 neurology & neurosurgery - Abstract
The plant cuticle is deposited on the surface of primary plant organs, such as leaves, fruits, and floral organs, forming a diffusion barrier and protecting the plant against various abiotic and biotic stresses. Cutin, the structural polyester of the plant cuticle, is synthesized in the apoplast. Plasma-membrane-localized ATP-binding cassette (ABC) transporters of the G family have been hypothesized to export cutin precursors. Here, we characterize SlABCG42 of tomato representing an ortholog of AtABCG32 in Arabidopsis. SlABCG42 expression in Arabidopsis complements the cuticular deficiencies of the Arabidopsis pec1/abcg32 mutant. RNAi-dependent downregulation of both tomato genes encoding proteins highly homologous to AtABCG32 (SlABCG36 and SlABCG42) leads to reduced cutin deposition and formation of a thinner cuticle in tomato fruits. By using a tobacco (Nicotiana benthamiana) protoplast system, we show that AtABCG32 and SlABCG42 have an export activity for 10,16-dihydroxy hexadecanoyl-2-glycerol, a cutin precursor in vivo. Interestingly, also free ω-hydroxy hexadecanoic acid as well as hexadecanedioic acid were exported, furthering the research on the identification of cutin precursors in vivo and the respective mechanisms of their integration into the cutin polymer.
- Published
- 2021
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