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Determination and interpretation of Wigner-like photoionisation delays is one of the most active fields of attosecond science. Previous results have suggested that large photoionisation delays are associated with structured continua, but a quantitative relation between photoionisation cross sections and time delays has been missing. Here, we derive a Kramers–Kronig-like relation between these quantities and demonstrate its validity for (anti)resonances. This new concept defines a topological analysis, which rationalises the sign of photoionisation delays and thereby sheds new light on a long-standing controversy regarding the sign of the photoionisation delay near the Ar 3 s Cooper minimum. Our work bridges traditional photoionisation spectroscopy with attosecond chronoscopy and offers new methods for analysing and interpreting photoionisation delays.

Abstract This meta-analysis aimed to analyze retinal microvasculature features in eyes with Behçet’s disease (BD) using optical coherence tomography angiography (OCTA). Electronic databases, including PubMed, Web of Science, Embase, and Cochrane Library, were comprehensively searched for published studies comparing retinal microvasculature characteristics between eyes with BD and controls. Continuous variables were calculated using the mean difference (MD) with 95% confidence interval (CI). Review Manager software (version 5.30) was used to conduct statistical analysis. A total of 13 eligible studies involving 599 eyes with BD and 622 control eyes were included in the meta-analysis. The pooled results showed that the macular whole enface superficial and deep vessel density (VD) values measured by OCTA were significantly lower in eyes with BD than in control eyes (superficial VD: MD = − 3.05, P

Hepatocyte transplantation and bioartificial liver (BAL) systems hold significant promise as less invasive alternatives to traditional transplantation, providing crucial temporary support for patients with acute and chronic liver failure. Although human hepatocytes are ideal, their use is limited by ethical concerns and donor availability, leading to the use of porcine hepatocytes in BAL systems due to their functional similarities. Recent advancements in gene‐editing technology have improved porcine organ xenotransplantation clinical trials by addressing immune rejection issues. Gene‐edited pigs, such as alpha‐1,3‐galactosyltransferase (GGTA1) knockout pigs, offer a secure source of primary cells for BAL systems. Our research focuses on optimizing the safety and functionality of porcine primary hepatocytes during large‐scale cultivation. We achieved this by creating GGTA1 knockout pigs through one‐step delivery of CRISPR/Cas9 to pig zygotes via oviduct injection of rAAV, and enhancing hepatocyte viability and function by co‐culturing hepatocytes with Roof plate‐specific spondin 1 overexpressing HUVECs (R‐HUVECs). Using a Rocker culture system, approximately 1010 primary porcine hepatocytes and R‐HUVECs rapidly formed organoids with a diameter of 92.1 ± 28.1 µm within 24 h. These organoids not only maintained excellent functionality but also supported partial hepatocyte self‐renewal during long‐term culture over 28 days. Gene‐edited primary porcine hepatocyte organoids will significantly advance the applications of hepatocyte transplantation and BAL systems. [ABSTRACT FROM AUTHOR]

AIM: To study the inhibition effect of TAK-242 on the proliferation of rat eye Tenon’s capsule fibroblasts via the toll-like receptor 4 (TLR4) signaling pathway. METHODS: SD rat Tenon’s capsule fibroblasts were extracted and cultured, then the cells were divided into normal control group, lipopolysaccharide (LPS) group (10 g/mL LPS) and TAK-242 group (1 μmol/L TAK-242, and 10 μg/mL LPS after 30min). The expressions of TLR4, transforming growth factor-β1 (TGF-β1) and interleukin-6 (IL-6) in each group were detected by Western blot and reverse transcriptase-polymerase chain reaction (RT-PCR). Cell proliferation was detected by cell counting kit-8 (CCK-8). RESULTS: Double immunofluorescent labeling in the extracted cells showed negative keratin staining and positive vimentin staining. Western blot showed that the LPS group had the highest expression of TLR4 and TGF-β1 (P0.05). RT-PCR showed that the IL-6 mRNA expression in LPS group was the highest in the three groups (P

In the world's first pig-to-human cardiac cytomegalovirus (PCMV), xenotransplant and elevated levels of porcine key factors contributing to patient mortality were considered. This has renewed attention on PCMV, a virus widely prevalent in pigs. Currently, there are no effective drugs or vaccines targeting PCMV, and its high detection difficulty poses challenges for prevention and control research. In this study, antiviral small hairpin RNA (shRNA) was selected and inserted into the Rosa26 and miR-17-92 loci of pigs via a CRISPR/Cas9-mediated knock-in strategy. Further in vitro viral challenge experiments demonstrated that these genetically edited pig cells could effectively limit PCMV replication. Through this process, we constructed a PCMV-infected cell model, validated partial viral interference sites, enhanced gene knock-in efficiency, performed gene editing at two different gene loci, and ultimately demonstrated that RNA interference (RNAi) technology combined with CRISPR/Cas9 has the potential to generate pig cells with enhanced antiviral infection capabilities. This opens up possibilities for the future production of pig populations with antiviral functionalities. [ABSTRACT FROM AUTHOR]