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3 results on '"Baoyu Mai"'

1. Rhoifolin Alleviates Alcoholic Liver Disease In Vivo and In Vitro via Inhibition of the TLR4/NF-κB Signaling Pathway

Author

Baoyu Mai, Ling Han, Jiarui Zhong, Jingqi Shu, Zelin Cao, Jiaqi Fang, Xiaoying Zhang, Zelin Gao, and Fengxia Xiao

Subjects
rhoifolin, ALD, LO2 cell, NF-κB, CYP2E1, Therapeutics. Pharmacology, RM1-950
Abstract

Background: Alcoholic liver disease (ALD) is a common chronic liver disorder worldwide, which is detrimental to human health. A preliminary study showed that the total flavonoids within Citrus grandis “Tomentosa” exerted a remarkable effect on the treatment of experimental ALD. However, the active substances of Citrus grandis “Tomentosa” were not elucidated. Rhoifolin (ROF) is a flavonoid component present in high levels. Therefore, this research aimed to evaluate the hepatoprotective effects of ROF and its possible mechanisms.Methods: Molecular docking was performed to analyze the binding energy of ROF to the main target proteins related to ALD. Subsequently, mice were fed ethanol (ETH) for 49 days to establish the chronic alcoholic liver injury models. The liver pathological injury, serum aminotransferase levels, and oxidative stress levels in the liver tissue were measured. Human normal hepatocytes (LO2 cells) were incubated with ETH to construct the alcoholic liver cell model. The inflammatory markers and apoptosis factors were evaluated using real-time PCR and flow cytometry. Finally, the effects of ROF on the CYP2E1 and NF-κB signaling pathways were tested in vitro and in vivo.Results: Molecular docking results demonstrated that ROF was able to successfully dock with the target proteins associated with ALD. In animal studies, ROF attenuated ETH-induced liver damage in mice by decreasing the serum concentrations of AST and ALT, reducing the expression of inflammatory cytokines, and maintaining antioxidant balance in the liver tissue. The in vitro experiments demonstrated that ROF suppressed ETH-induced apoptosis in LO2 cells by promoting Bcl-2 mRNA and inhibiting Bax mRNA and caspase 3 protein expression. ROF decreased the level of LDH, ALT, AST, ROS, and MDA in the supernatant; induced the activity of GSH and SOD; and inhibited TNF-α, IL-6, and IL-1β expression levels. Mechanistically, ROF could significantly downregulate the expression levels of CYP2E1, TLR4, and NF-κB phosphorylation.Conclusion: This study indicates that ROF is the active component within the total flavonoids, which may alleviate ETH-induced liver injury by inhibiting NF-κB phosphorylation. Therefore, ROF may serve as a promising compound for treating ALD.

Published
2022
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2. Rhoifolin Alleviate Alcoholic Liver Disease by Inhibiting the TLR4 / NF-kB Signaling Pathway and Attenuating Oxidative Stress

Author

Xiaoying Zhang, Baoyu Mai, Zelin Cao, Jiarui Zhong, Zelin Gao, Jiaqi Fang, Fengxia Xiao, and Ling Han

Subjects
chemistry.chemical_compound, Alcoholic liver disease, Rhoifolin, chemistry, NF-kB Signaling Pathway, medicine, TLR4, Pharmacology, medicine.disease, medicine.disease_cause, Oxidative stress
Abstract

Alcoholic liver disease (ALD) is caused by long-term excessive consumption of alcohol, which is affected by TLR4 / NF-kB mediated inflammatory response and CYP2E1-mediated oxidative stress effects. Rhoifolin (ROF) is a flavonoid compound in Citrus grandis ‘Tomentosa’, with antioxidant and anti-inflammation effect. However, the action of its effect on ALD has been elucidated yet. In the present study, we investigated ROF’s anti-inflammatory, antioxidant and antiapoptotic action in the treatment of ALD. In established ALD mice, ROF promoted hepatic function through downgrading the amount of aminotransferase, attenuating oxidative stress, and restoring antioxidant balance in hepatic tissue. Additionally, ROF significantly reduced the expression of inflammatory cytokines, such as NF-kB, TNF-a, IL-6, and IL-1b in the mice. In vitro experiments indicated that ROF increased the LO2 cells viability and inhibited cells apoptosis. ROF reversed the expression of CYP2E1, NLRP3, p-p65, p-IkB and TLR4, which were consistent with animal experiment. Overall, ROF can alleviate ethanol-induced liver injury through inhibiting oxidative stress and inflammation, and is a promising compound for ALD treatment.

Published
2021
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3. Rhoifolin Alleviates Inflammation of Acute Inflammation Animal Models and LPS-Induced RAW264.7 Cells via IKKβ/NF-κB Signaling Pathway

Author

Tingfang Wen, Xiaoxin Song, Jiaqi Fang, Taojunfeng Su, Xiaoying Zhang, Baoyu Mai, Zelin Cao, Jingran Lin, Fengxia Xiao, Jialan Chen, and Yuguang Chi

Subjects
0301 basic medicine, Lipopolysaccharides, Citrus, Lipopolysaccharide, Cell Survival, Immunology, Inflammation, CCL2, Pharmacology, Carrageenan, Disaccharides, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Western blot, medicine, Immunology and Allergy, Animals, Edema, Viability assay, Glycosides, Phosphorylation, Flavonoids, medicine.diagnostic_test, NF-kappa B, I-kappa B Kinase, Rats, IκBα, 030104 developmental biology, RAW 264.7 Cells, chemistry, 030220 oncology & carcinogenesis, Cytokines, medicine.symptom, Signal transduction, Signal Transduction
Abstract

Rhoifolin (ROF) is a main effective component in Citrus grandis 'Tomentosa'. ROF has a potential anti-inflammatory activity, but its specific effects and mechanisms have not been studied. This study investigated the anti-inflammatory activity of ROF and searched for its possible molecular mechanisms. A mouse model of acute inflammation was induced by lipopolysaccharide, and the effects of ROF on pathological damages of the lung and liver were observed. Carrageenan-induced paw edema rat model was used to evaluate the effect of ROF on the volume of swelling paw. In LPS-induced RAW264.7 macrophages, the expression levels of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α were measured using ELISA. Real-time PCR was used to measure the mRNA levels of iNOS and CCL2. Western blot was used to detect the activation of IκBα and IKKβ in NF-κB signaling pathways. The results showed that ROF accelerated the recoveries of liver and lung tissue damages in acute inflammation mice and inhibited carrageenan-induced paw edema in rats; in addition, ROF significantly suppressed the secretion of TNF-α, IL-1β, and IL-6 in the serum of rats and mouse model. In LPS-induced RAW264.7 cells, 100 μmol/L ROF enhanced cell viability and suppressed the production of TNF-α, IL-6, and IL-1β significantly. ROF also decreased the mRNA expression of iNOS and CCL2 and inhibited IκBα and IKKβ phosphorylation. In summary, ROF had a potential therapeutic value for inflammation. Our research provided experimental basis for the further development of ROF as an anti-inflammatory drug and for clarifying the anti-inflammatory substance basis of Citrus grandis 'Tomentosa'. Graphical Abstract.

Published
2020

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