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Radical prostatectomy is the best treatment for patients with T1-2 prostate cancer and a life expectancy of over 10 years, but no pN1 patient that underwent only surgery, was progression free at 10 years (Partin 1994). In the last 10 years we performed 441 consecutive pelvic lymphadenectomy in patients with prostate cancer. We examined 32 patients pN1 (7.2%), with preoperatory PSA < 10 ng/ml. Mean age was 66.7, all these patients were clinically T1-2, mean PSA was 6.89 ng/ml and mean Gleason score 6.7 (< or = 7 in 27/32). Frozen sections and orchiectomy were performed in 8 patients who had macroscopic nodal involvement. In 24 patients with microscopic nodal involvement retropubic radical prostatectomy was performed. Pathologic stadiation (TNM 1997) was: 1 pT2a N1, 6 pT2b N1, 2 pT3a N1, 14 pT3b N1 and 1 pT4 N1. Seminal vesicle involvement was found in 14 of patients; in 13 of these positive surgical margins was found. Definitive Gleason score was > or = 7 in 22/24 patients (mean 7.7). Five patients underwent adjuvant radiotherapy, 13 radiotherapy and hormonotherapy and 6 only hormonotherapy. Mean follow up was at 66.6 months, but data are available for only 29/32 patients, with 8 (27.5%) biochemical progressions and 2 deaths (10.3%) for metastatic disease. Our experience indicates that the pelvic lymphadenectomy is very important for correct staging, also when PSA <10 ng/mI, bioptic Gleason score < or = 7 and clinical stage T1-2. In pN1 patients the adjuvant hormonotherapy allows a significant extension of disease free survival. [ABSTRACT FROM AUTHOR]

(High intensity focused ultrasound) HIFU is a minimally invasive treatment based on thermal ablation of tissues which are warmed up to 85°C in the focal area. From May 2002 to March 2008, 145 patients with prostate cancer, aged 44 to 86 years (mean 74.4) underwent 170 HIFU treatments. Mean PSA was 28. 1 ng/ml (range 0, 1-143) and mean Gleason sum 6.3 (range 3-9). The prognosis subgroups were defined as low-risk in 29 patients (clinical stage T1-T2a, PSA < or = 10 ng/mL, and Gleason score lower than 7), intermediate-risk in 21 patients (clinical stage T2b or PSA 10 - 20 ng/mL, or Gleason score of 7), and high-risk in 95 patients (clinical stage > or = T2c, or PSA > 20 ng/mL, or Gleason score higher than 7). At a mean follow-up of 47.1 months, biochemical success rate (PSA constantly <0.5 ng/ml) was obtained in 84.0% of low and intermediate risk patients and in 43. 1% of high risk patients; post-treatment biopsies (6 months after treatment) revealed no residual tumor in 92.8% of low or intermediate risk patients and in 62.3 % of high risk patients. Complication included: asymptomatic urinary tract infection (17.5%), pros tatitis (2.9%), epididymorchitis (1.7%), hemorrhoidal pain (0.5%), hematuria (3.5%), prostatic urethra structures (7.6%%), bladder neck sclerosis (12.3%) and rectourethral fistula (0.5%). Light stress incontinence occurred in 4.1% of the patients and erectile dysfunction in 77.8%. HIFU seems to be a promising alternative and less invasive treatment modality with an encouraging success rate, at least in the short-term. [ABSTRACT FROM AUTHOR]

Laparoscopic radical nephrectomy is a standard procedure performed in many institutes. Major vascular complications, especially vena cava injury, are uncommon but serious. Early recognition and conversion to open procedure may be required. We report a case of vena cava injury during transperitoneal laparoscopic radical nephrectomy managed laparoscopically by a surgeon with an extensive experience with intracorporeal suturing, who operated at our institute as a tutor. Major vascular injuries during Iaparoscopic surgery are rare, with an incidence of 0.05%; they are due to malfunction of the instruments, errors made during dissection, misplacement of stapling devices during ligation and disorientation of the surgical field. In literature only two cases of vena cava transection during laparoscopic nepherectomy are reported. The result of these injuries is usually hemorrhage; traditional therapy is emergency laparotomy, consultation with a vascular surgeon and open repair, especially during retroperitoneal laparoscopy, where the available working space is limited and creates a special challenge for control of vascular accidents and performance of laparoscopic suturing maneuvers. The occurrence of major vascular accidents during laparoscopy and the rate of open conversion are related to operator. Vena cava injury during laparoscopic radical nephrectomy is a highly unlikely but potentially devastating complication, that in selected circumstances, can be managed laparoscopically only by surgeons with an extensive experience especially in intracorporeal suturing. [ABSTRACT FROM AUTHOR]

Nephroureterectomy with excision of a cuff of bladder remains the standard for managing upper tract transitional cell carcinoma, which could, in selected cases, be managed conservatively. In 10 years we have treated 67 reno-ureteral unities (r.u.u.) for urothehal tumors; 36 r.u.u. with low-stage and low-grade disease (28 patients, 8 with bilateral tumor and 2 solitary kidney), were treated by an endoscopic approach; in 34 cases (3 for high grade recurrences after conservative approach) nephroureterectomy was performed. In 36 r.u.u. treated by ureteroscopic approach, we observed 3 high-grade recurrences (nephroureterectomy) and 22 low-grade (G1-G2) recurrences. Each r.u.u. received an average of 2.08 ureteroscopic operative procedures. The patients were followed up fora mean of 47.6 months after initial treatment and currently they are all recurrence free. Nine patients (13,4%) with suspect tumor of the upper tract had no carcinoma in the ureteroscopic biopsy. Ureteroscopic approach of upper urinary tract urothelial tumors in the current literature has been used successfully, resulting in recurrence rates ranging from 31% to 65% and disease-free rates of 35% to 86%. Progression and metastatic rates are low and correlate with tumor grade. [ABSTRACT FROM AUTHOR]

Flat lenses with focal length tunability can enable the development of highly integrated imaging systems. This work explores machine learning to inverse design a multifocal multilevel diffractive lens (MMDL) by wavelength multiplexing. The MMDL output is multiplexed in three color channels, red (650 nm), green (550 nm), and blue (450 nm), to achieve varied focal lengths of 4 mm, 20 mm, and 40 mm at these three color channels, respectively. The focal lengths of the MMDL scale significantly with the wavelength in contrast to conventional diffractive lenses. The MMDL consists of concentric rings with equal widths and varied heights. The machine learning method is utilized to optimize the height of each concentric ring to obtain the desired phase distribution so as to achieve varied focal lengths multiplexed by wavelengths. The designed MMDL is fabricated through a direct-write laser lithography system with gray-scale exposure. The demonstrated singlet lens is miniature and polarization insensitive, and thus can potentially be applied in integrated optical imaging systems to achieve zooming functions.

Machine learning can efficiently empower the inverse design of cascaded diffractive optical elements. In this work, we explore the inverse design of a bidirectional focusing diffractive lens in a cascaded configuration through the diffractive optical neural network (DONN) machine learning method. The bidirectional focusing diffractive lens consists of two on-axially cascaded multi-level diffractive lenses. Each lens consists of concentric rings with equal widths and varying heights. The height of each concentric ring is optimized as part of the design algorithm. The diffractive lens has a focal length f + as light propagates in the forward (Z+) direction. As light propagates in the backward (Z-) direction, the focal length changes to f - . The designed lens is fabricated through a two-photon polymerization 3D printing technique. The proposed design is polarization insensitive and miniature and can be readily applied in future functional optical imaging systems.

Multilayer diffractive optical neural networks (DONNs) can perform machine learning (ML) tasks at the speed of light with low energy consumption. Decreasing the number of diffractive layers can reduce inevitable material and diffraction losses to improve system performance, and incorporating compact devices can reduce the system footprint. However, current analytical DONN models cannot accurately describe such physical systems. Here we show the ever-ignored effects of interlayer reflection and interpixel interaction on the deployment performance of DONNs through full-wave electromagnetic simulations and terahertz (THz) experiments. We demonstrate that the drop of handwritten digit classification accuracy due to reflection is negligible with conventional low-index THz polymer materials, while it can be substantial with high-index materials. We further show that one- and few-layer DONN systems can achieve high classification accuracy, but there is a trade-off between accuracy and model-system matching rate because of the fast-varying spatial distribution of optical responses in diffractive masks. Deep DONNs can break down such a trade-off because of reduced mask spatial complexity. Our results suggest that new accurate and trainable DONN models are needed to advance the development and deployment of compact DONN systems for sophisticated ML tasks.

In this paper, we discuss flat programmable multi-level diffractive lenses (PMDL) enabled by phase change materials working in the near-infrared and visible ranges. The high real part refractive index contrast (Δn ∼ 0.6) of Sb 2 S 3 between amorphous and crystalline states, and extremely low losses in the near-infrared, enable the PMDL to effectively shift the lens focus when the phase of the material is altered between its crystalline and amorphous states. In the visible band, although losses can become significant as the wavelength is reduced, the lenses can still provide good performance as a result of their relatively small thickness (∼ 1.5λ to 3λ). The PMDL consists of Sb 2 S 3 concentric rings with equal width and varying heights embedded in a glass substrate. The height of each concentric ring was optimized by a modified direct binary search algorithm. The proposed designs show the possibility of realizing programmable lenses at design wavelengths from the near-infrared (850 nm) up to the blue (450 nm) through engineering PMDLs with Sb 2 S 3 . Operation at these short wavelengths, to the best of our knowledge, has not been studied so far in reconfigurable lenses with phase-change materials. Therefore, our results open a wider range of applications for phase-change materials, and show the prospect of Sb 2 S 3 for such applications. The proposed lenses are polarization insensitive and can have the potential to be applied in dual-functionality devices, optical imaging, and biomedical science.

In this work, a systematic photoluminescence (PL) study on three series of gallium oxide/aluminum gallium oxide films and bulk single crystals is performed including comparing doping, epitaxial substrates, and aluminum concentration. It is observed that blue/green emission intensity strongly correlates with extended structural defects rather than the point defects frequently assumed. Bulk crystals or Si-doped films homoepitaxially grown on (010) β-Ga 2 O 3 yield an intense dominant UV emission, while samples with extended structural defects, such as gallium oxide films grown on either (-201) β-Ga 2 O 3 or sapphire, as well as thick aluminum gallium oxide films grown on either (010) β-Ga 2 O 3 or sapphire, all show a very broad PL spectrum with intense dominant blue/green emission. PL differences between samples and the possible causes of these differences are analyzed. This work expands previous reports that have so far attributed blue and green emissions to point defects and shows that in the case of thin films, extended defects might have a prominent role in emission properties., (© 2022. The Author(s).)

We designed, fabricated, and characterized a flat multi-level diffractive lens comprised of only silicon with d i a m e t e r =15.2 m m , focal l e n g t h =19 m m , numerical aperture of 0.371, and operating over the long-wave infrared (LWIR) s p e c t r u m =8µ m to 14 µm. We experimentally demonstrated a field of view of 46°, depth of focus >5 m m , and wavelength-averaged Strehl ratio of 0.46. All of these metrics were comparable to those of a conventional refractive lens. The active device thickness is only 8 µm, and its weight (including the silicon substrate) is less than 0.2 g.

It is generally assumed that correcting chromatic aberrations in imaging requires multiple optical elements. Here, we show that by allowing the phase in the image plane to be a free parameter, it is possible to correct chromatic variation of focal length over an extremely large bandwidth, from the visible (Vis) to the longwave infrared (LWIR) wavelengths using a single diffractive surface, i.e., a flat lens. Specifically, we designed, fabricated and characterized a flat, multi-level diffractive lens (MDL) with a thickness of ≤ 10µm, diameter of ∼1mm, and focal length of 18mm, which was constant over the operating bandwidth of λ=0.45µm (blue) to 15µm (LWIR). We experimentally characterized the point-spread functions, aberrations and imaging performance of cameras comprised of this MDL and appropriate image sensors for λ=0.45μm to 11μm. We further show using simulations that such extreme achromatic MDLs can be achieved even at high numerical apertures (NA=0.81). By drastically increasing the operating bandwidth and eliminating several refractive lenses, our approach enables thinner, lighter and simpler imaging systems.

Deep neural networks (DNNs) have substantial computational requirements, which greatly limit their performance in resource-constrained environments. Recently, there are increasing efforts on optical neural networks and optical computing based DNNs hardware, which bring significant advantages for deep learning systems in terms of their power efficiency, parallelism and computational speed. Among them, free-space diffractive deep neural networks (D 2 NNs) based on the light diffraction, feature millions of neurons in each layer interconnected with neurons in neighboring layers. However, due to the challenge of implementing reconfigurability, deploying different DNNs algorithms requires re-building and duplicating the physical diffractive systems, which significantly degrades the hardware efficiency in practical application scenarios. Thus, this work proposes a novel hardware-software co-design method that enables first-of-its-like real-time multi-task learning in D 2 2NNs that automatically recognizes which task is being deployed in real-time. Our experimental results demonstrate significant improvements in versatility, hardware efficiency, and also demonstrate and quantify the robustness of proposed multi-task D 2 NN architecture under wide noise ranges of all system components. In addition, we propose a domain-specific regularization algorithm for training the proposed multi-task architecture, which can be used to flexibly adjust the desired performance for each task.

While terahertz spectroscopy can provide valuable information regarding the charge transport properties in semiconductors, its application for the characterization of low-conductive two-dimensional layers, i.e., σ s  <  < 1 mS, remains elusive. This is primarily due to the low sensitivity of direct transmission measurements to such small sheet conductivity levels. In this work, we discuss harnessing the extraordinary optical transmission through gratings consisting of metallic stripes to characterize such low-conductive two-dimensional layers. We analyze the geometric tradeoffs in these structures and provide physical insights, ultimately leading to general design guidelines for experiments enabling non-contact, non-destructive, highly sensitive characterization of such layers.

Compound eyes found in insects provide intriguing sources of biological inspiration for miniaturized imaging systems. Inspired by such insect eye structures, we demonstrate an ultrathin arrayed camera enabled by a flat multi-level diffractive microlens array for super-resolution visible imaging. We experimentally demonstrate that the microlens array can achieve a large fill factor (hexagonal close packing with p i t c h =120µ m ), thickness of 2.6 µm, and diffraction-limited ( S t r e h l r a t i o =0.88) achromatic performance in the visible band (450 to 650 nm). We also demonstrate super-resolution imaging with resolution improvement of ∼1.4 times by computationally merging 1600 images in the array.

Multilevel diffractive lenses (MDLs) have emerged as an alternative to both conventional diffractive optical elements (DOEs) and metalenses for applications ranging from imaging to holographic and immersive displays. Recent work has shown that by harnessing structural parametric optimization of DOEs, one can design MDLs to enable multiple functionalities like achromaticity, depth of focus, wide-angle imaging, etc. with great ease in fabrication. Therefore, it becomes critical to understand how fabrication errors still do affect the performance of MDLs and numerically evaluate the trade-off between efficiency and initial parameter selection, right at the onset of designing an MDL, i.e., even before putting it into fabrication. Here, we perform a statistical simulation-based study on MDLs (primarily operating in the THz regime) to analyse the impact of various fabrication imperfections (single and multiple) on the final structure as a function of the number of ring height levels. Furthermore, we also evaluate the performance of these same MDLs with the change in the refractive index of the constitutive material. We use focusing efficiency as the evaluation criterion in our numerical analysis; since it is the most fundamental property that can be used to compare and assess the performance of lenses (and MDLs) in general designed for any application with any specific functionality.

In Opt. Lett.44, 5450 (2019)OPLEDP0146-959210.1364/OL.44.005450, there were errors in the author listing and in one figure.

We experimentally demonstrate a ∼1-mm-thick near infrared camera comprised of a multi-level diffractive lens coupled with a conventional monochrome image sensor. We performed careful measurements of the point-spread function, the modulation transfer function, focusing efficiency, aberrations, and the field of view of the camera.

We experimentally demonstrate imaging in the long-wave infrared (LWIR) spectral band (8 μm to 12 μm) using a single polymer flat lens based upon multilevel diffractive optics. The device thickness is only 10 μm, and chromatic aberrations are corrected over the entire LWIR band with one surface. Due to the drastic reduction in device thickness, we are able to utilize polymers with absorption in the LWIR, allowing for inexpensive manufacturing via imprint lithography. The weight of our lens is less than 100 times those of comparable refractive lenses. We fabricated and characterized 2 different flat lenses. Even with about 25% absorption losses, experiments show that our flat polymer lenses obtain good imaging with field of view of 35° and angular resolution less than 0.013°. The flat lenses were characterized with 2 different commercial LWIR image sensors. Finally, we show that, by using lossless, higher-refractive-index materials like silicon, focusing efficiencies in excess of 70% can be achieved over the entire LWIR band. Our results firmly establish the potential for lightweight, ultrathin, broadband lenses for high-quality imaging in the LWIR band., Competing Interests: Competing interest statement: R.M. is cofounder of Oblate Optics, Inc., which is commercializing technology discussed in this manuscript. The University of Utah has filed for patent protection for technology discussed in this manuscript., (Copyright © 2019 the Author(s). Published by PNAS.)

Three-dimensional (3D) semimetals have been predicted and demonstrated to have a wide variety of interesting properties associated with their linear energy dispersion. In analogy to two-dimensional (2D) Dirac semimetals, such as graphene, Cd 3 As 2 has shown ultrahigh mobility and large Fermi velocity and has been hypothesized to support plasmons at terahertz frequencies. In this work, we experimentally demonstrate synthesis of high-quality large-area Cd 3 As 2 thin films through thermal evaporation as well as the experimental realization of plasmonic structures consisting of periodic arrays of Cd 3 As 2 stripes. These arrays exhibit sharp resonances at terahertz frequencies with associated quality factors ( Q) as high as ∼3.7 (at 0.82 THz). Such spectrally narrow resonances can be understood on the basis of a long momentum scattering time, which in our films can approach ∼1 ps at room temperature. Moreover, we demonstrate an ultrafast tunable response through excitation of photoinduced carriers in optical pump/terahertz probe experiments. Our results evidence that the intrinsic 3D nature of Cd 3 As 2 might provide for a very robust platform for terahertz plasmonic applications. Moreover, the long momentum scattering time as well as large kinetic inductance in Cd 3 As 2 also holds enormous potential for the redesign of passive elements such as inductors and hence can have a profound impact in the field of RF integrated circuits.

We demonstrate ultra-thin (1.5-3λ 0 ), fabrication-error tolerant efficient diffractive terahertz (THz) optical elements designed using a computer-aided optimization-based search algorithm. The basic operation of these components is modeled using scalar diffraction of electromagnetic waves through a pixelated multi-level 3D-printed polymer structure. Through the proposed design framework, we demonstrate the design of various ultrathin planar THz optical elements, namely (i) a high Numerical Aperture (N.A.), broadband aberration rectified spherical lens (0.1 THz-0.3 THz), (ii) a spectral splitter (0.3 THz-0.6 THz) and (iii) an on-axis broadband transmissive hologram (0.3 THz-0.5 THz). Such an all-dielectric computational design-based approach is advantageous against metallic or dielectric metasurfaces from the perspective that it incorporates all the inherent structural advantages associated with a scalar diffraction based approach, such as (i) ease of modeling, (ii) substrate-less facile manufacturing, (iii) planar geometry, (iv) high efficiency along with (v) broadband operation, (vi) area scalability and (vii) fabrication error-tolerance. With scalability and error tolerance being two major bottlenecks of previous design strategies. This work is therefore, a significant step towards the design of THz optical elements by bridging the gap between structural and computational design i.e. through a hybrid design-based approach enabling considerably less computational resources than the previous state of the art. Furthermore, the approach used herein can be expanded to a myriad of optical elements at any wavelength regime.

We report polarization dependent photoluminescence studies on unintentionally-, Mg-, and Ca-doped β-Ga 2 O 3 bulk crystals grown by the Czochralski method. In particular, we observe a wavelength shift of the highest-energy UV emission which is dependent on the pump photon energy and polarization. For 240 nm (5.17 eV) excitation almost no shift of the UV emission is observed between E||b and E||c, while a shift of the UV emission centroid is clearly observed for 266 nm (4.66 eV), a photon energy lying between the band absorption onsets for the two polarizations. These results are consistent with UV emission originating from transitions between conduction band electrons and two differentially-populated self-trapped hole (STH) states. Calcuations based on hybrid and self-interaction-corrected density functional theories further validate that the polarization dependence is consistent with the relative stability of two STHs. This observation implies that the STHs form primarily at the oxygen atoms involved in the original photon absorption event, thus providing the connection between incident polarization and emission wavelength. The data imposes a lower bound on the energy separation between the self-trapped hole states of ~70-160 meV, which is supported by the calculations.

We report on terahertz characterization of La-doped BaSnO 3 (BSO) thin-films. BSO is a transparent complex oxide material, which has attracted substantial interest due to its large electrical conductivity and wide bandgap. The complex refractive index of these films is extracted in the 0.3 to 1.5 THz frequency range, which shows a metal-like response across this broad frequency window. The large optical conductivity found in these films at terahertz wavelengths makes this material an interesting platform for developing electromagnetic structures having a strong response at terahertz wavelengths, i.e. terahertz-functional, while being transparent at visible and near-IR wavelengths. As an example of such application, we demonstrate a visible-transparent terahertz polarizer.

We characterize the terahertz (THz) magneto-plasmonic response of a cobalt-based periodic aperture array. The bare cobalt surface allows for low loss propagation of surface plasmon-polaritons, as evidenced by comparing the reflection from aperture arrays coated with Au and with Co. When an external magnetic field is applied in a polar Kerr geometry, we observe a maximum polarization rotation of ~0.6° and an ellipticity of ~0.35° from the Co-based array. These values are larger than expected based on existing models that include only interband transitions in ferromagnetic metals. We discuss possible reasons for the difference between experiment and theory.

In this work we study the terahertz light propagation through deeply-scaled graphene-based reconfigurable metasurfaces, i.e. metasurfaces with unit-cell dimensions much smaller than the terahertz wavelength. These metasurfaces are analyzed as phase modulators for constructing reconfigurable phase gradients along an optical interface for the purpose of beam shaping. Two types of deeply-scaled metacell geometries are analyzed and compared, which consist of: (i) multi split ring resonators, and (ii) multi spiral resonators. Two figures of merit, related to: (a) the loss and (b) the degree of reconfigurability achievable by such metamaterials -when applied in beam shaping applications-, are introduced and discussed. Simulations of these two types of deep-subwavelength geometries, when changing the metal coverage-fraction, show that there is an optimal coverage-fraction that gives the best tradeoff in terms of loss versus degree of reconfigurability. For both types of geometries the best tradeoff occurs when the area covered by the metallic region is around 40% of the metacell total area. From this point of view, reconfigurable deeply-scaled metamaterials can indeed provide a superior performance for beam shaping applications when compared to not deeply-scaled ones; however, counterintuitively, employing very highly-packed structures might not be beneficial for such applications.

In this paper we propose and experimentally demonstrate arrays of graphene electro-absorption modulators as electrically reconfigurable patterns for terahertz cameras. The active element of these modulators consists of only single-atom-thick graphene, achieving a modulation of the THz wave reflectance > 50% with a potential modulation depth approaching 100%. Although the prototype presented here only contains 4x4 pixels, it reveals the possibility of developing reliable low-cost video-rate THz imaging systems employing single detector.

Switchable metamaterials offer unique solutions for efficiently manipulating electromagnetic waves, particularly for terahertz waves, which has been difficult since naturally occurring materials rarely respond to terahertz frequencies controllably. However, few terahertz modulators demonstrated to date exhibit simultaneously low attenuation and high modulation depth. In this letter we propose a new class of electrically-tunable terahertz metamaterial modulators employing metallic frequency-selective-surfaces (FSS) in conjunction with capacitively-tunable layers of electrons, promising near 100% modulation depth and < 15% attenuation. The fundamental departure in our design from the prior art is tuning enabled by self-gated electron layers that is independent from the metallic FSS. Our proposal is applicable to all possible electrically tunable elements including graphene, Si, MoS(2), oxides etc, thus opening up myriad opportunities for realizing high performance switchable metamaterials over an ultra-wide terahertz frequency range.

We demonstrate a graphene-based electro-absorption modulator achieving extraordinary control of terahertz reflectance. By concentrating the electric field intensity in an active layer of graphene, an extraordinary modulation depth of 64% is achieved while simultaneously exhibiting low insertion loss (∼2 dB), which is remarkable since the active region of the device is atomically thin. This modulator performance, among the best reported to date, indicates the enormous potential of graphene for terahertz reconfigurable optoelectronic devices.

Terahertz technology promises myriad applications including imaging, spectroscopy and communications. However, one major bottleneck at present for advancing this field is the lack of efficient devices to manipulate the terahertz electromagnetic waves. Here we demonstrate that exceptionally efficient broadband modulation of terahertz waves at room temperature can be realized using graphene with extremely low intrinsic signal attenuation. We experimentally achieved more than 2.5 times superior modulation than prior broadband intensity modulators, which is also the first demonstrated graphene-based device enabled solely by intraband transitions. The unique advantages of graphene in comparison to conventional semiconductors are the ease of integration and the extraordinary transport properties of holes, which are as good as those of electrons owing to the symmetric conical band structure of graphene. Given recent progress in graphene-based terahertz emitters and detectors, graphene may offer some interesting solutions for terahertz technologies.

We describe a new method to detect trace levels of ptaquiloside (Pta), a major carcinogen of bracken fern in biological samples such as milk from farm animals. The method involves the absorption of analyte on carbograph followed by elution with solvents mixtures. The unstable analyte is then converted into Br-Pt (II), which is specific for Pta, as it is not a natural decay product of the glycoside in aqueous media. An internal standard, the Br-pterosine-d 2 , prepared in our laboratories has been used. Detection and quantification are possible with gas chromatography/mass spectrometry (GC/MS) in single ion monitoring mode (SIM). The detectable amount is in the range of ppb. The method allowed us to detect Pta not only in the milk from bracken fern-poisoned cattle but also, for the first time, in the milk from healthy farm animals such as sheep, goat, horse, and donkey mares., (Copyright © 2010 Elsevier Ltd. All rights reserved.)

Objective: High-intensity focused ultrasound (HIFU) is a minimally invasive treatment based on thermal ablation of tissues which are warmed up to 85 degrees C in the focal area. Clinical studies have shown such treatment modality to be safe and effective in the management of localised prostate cancer as well as of local recurrences after radical prostatectomy or radiotherapy., Material and Methods: From May 2002 to June 2010, 171 patients with no previous treatment for prostate cancer, aged 44 to 86 years (mean 74.7) underwent 197 HIFU treatments; 22 patients needed a second treatment as the first was incomplete (4 patients) or because of recurrence (18 patients). The prognosis subgroups were defined as low-risk in 29 patients (clinical stage T1-T2a, PSA < or = 10 ng/mL and Gleason score lower than 7), intermediate-risk in 47 patients (clinical stage T2b or PSA 10 - 20 ng/mL or Gleason score of 7), and high-risk in 95 patients (clinical stage > or = T2c or PSA > 20 ng/mL or Gleason score higher than 7)., Results: At a mean follow-up of 67.9 months, biochemical success rate (PSA constantly < 0.5 ng/ml) was obtained in 84.2% of low and intermediate risk patients and in 43.1% of high risk patients; post-treatment biopsies (6 months after treatment) revealed no residual tumour in 93.4% of low or intermediate risk patients and in 63.1% of high risk patients., Conclusions: Radical prostatectomy remains the "gold standard" for localised prostate cancer. However, HIFU seems to be a promising alternative and less invasive treatment modality with an encouraging success rate, at least in the short-term, in patients with low and medium risk of progression, not candidates for radical surgery; in cancers with clinical stage > or = T2c, or PSA > 20 ng/mL, or Gleason score higher than 7 seems to get good results in about half of patients.

In human cells the length of telomeres depends on telomerase activity. This activity and the expression of the catalytic subunit of human telomerase reverse transcriptase (hTERT) is strongly up-regulated in most human cancers. hTERT expression is regulated by different transcription factors, such as c-Myc, Mad1 and Sp1. In this study, we demonstrated that 15d-PG J2 and rosiglitazone (an endogenous and synthetic peroxisome proliferators activated receptor gamma (PPARgamma) ligand, respectively) inhibited hTERT expression and telomerase activity in CaCo-2 colon cancer cells. Moreover, both ligands inhibited c-Myc protein expression and its E-box DNA binding activity. Additionally, Mad1 protein expression and its E-box DNA binding activity were strongly increased by 15d-PG J2 and, to a lesser extent, by rosiglitazone. Sp1 transcription factor expression and its GC-box DNA binding activity were not affected by both PPARgamma ligands. Results obtained by transient transfection of CaCo-2 cells with pmaxFP-Green-PRL plasmid constructs containing the functional hTERT core promoter (including one E-box and five GC-boxes) and its E-box deleted sequences, cloned upstream of the green fluorescent protein reporter gene, demonstrated that 15d-PG J2, and with minor effectiveness, rosiglitazone, strongly reduced hTERT core promoter activity. E-boxes for Myc/Mad/Max binding showed a higher activity than GC-boxes for Sp1. By using GW9662, an antagonist of PPARgamma, we demonstrated that the effects of 15d-PG J2 are completely PPARgamma independent, whereas the effects of rosiglitazone on hTERT expression seem to be partially PPARgamma independent. The regulation of hTERT expression by 15d-PG J2 and rosiglitazone, through the modulation of the Myc/Max/Mad1 network, may represent a new mechanism of action of these substances in inhibiting cell proliferation.

In medicinal chemistry field, the biochemical pathways, involved in 7-transmembrane domains G-protein coupled receptors (GPCRs) activation, are commonly studied to establish the activity of ligands towards GPCRs. The most studied steps are the measurement of activated GTP-alpha subunit and stimulated intracellular cAMP. At the present, many researchers defined agonist or antagonist activity of potential GPCRs drugs employing [(35)S]GTPgammaS or [(3)H]cAMP as probes. Recently, the corresponding lanthanide labels Eu-GTP and Eu-cAMP as alternative to radiochemicals have been developed because they are highly sensitive, easy to automate, easily synthesized, they display a much longer shelf-life and they can be used in multilabel experiments. In the present review, the receptor-drug interaction by europium employment for studying the biochemical pathway of GPCR activation has been focused. Moreover, comparative studies between lanthanide label probes and the corresponding radiolabeled compounds have been carried out.

Biliary tract cancer is a quite rare disease; despite recent significant advances in imaging modalities, most of the patients have advanced disease at presentation thus making radical surgery not feasible. Many different chemotherapeutic regimens have been investigated in small uncontrolled studies, with generally disappointing results. We extensively reviewed the literature on this topic trying to give an explanation to chemoresistance in this setting of patients and considering the molecular profiling as a tool for treatment decision. This review is divided in two parts, in the first one we illustrated chemotherapy results and possible mechanisms of resistance. In the second part we analysed the new molecular targets developing an hypothesis about the future therapeutics perspectives.

The echo color doppler allows an easy topographical distinction of the spermatic reflux (in front of the deferens) from cremasteric one (back to the deferens). This distinction consents a rational surgical choice, the selective interruption of the refluent vein and a less morbidity due to the surgical intervention. We evaluated 20 infertile patients (mean age 23 years) with echo color doppler testicular vessels (B & K 7.5 Mhz transducer). Seven varicoceles were clinically evident, 13 varicoceles were subclinic. After locating the deferens, we evaluated the venous reflux in the spermatic and cremasteric veins. The evaluation has been performed at rest and during Valsava maneuver. We used, in the correction of the anterior (spermatic) reflux, a retroperitoneal surgical access, while in the back (cremasteric) reflux isolated or combined, we performed middle or under inguinal access. The mean follow up has been of 4 months. In all cases the veins with reflux have been easily characterized. In 16 patients (80%) the spermatic vein was concerned; in an isolated case (5%) the reflux concerned only the cremasteric vein, in 3 patients (15%), with clinical varicocele, the reflux was present in both the veins (Coolsaet III type). In the 16 spermatic varicoceles, treated with retroperitoneal access (Palomo), the echo color doppler check demonstrated the absence of venous reflux. In the 4 varicoceles regarding the isolated or associate cremasteric vein treated with middle or under inguinal access, the existence of extra funicular varix was highlighted and after surgery the echo color doppler check was negative. In our few cases the echo color doppler diagnosis demonstrated a total correspondence with the surgical finds and with the doppler follow up. This diagnostic approach allows to reserve the retroperitoneal surgery to the spermatic pure reflux avoiding the false relapses due to the persistence of an undiagnosed cremasteric reflux. The middle or under inguinal approaches, generally loaded from a greatest morbidity, could be only employed in presence of an isolated or combined cremasteric reflux.

Indoor climate indices and microbiological airborne contamination were evaluated in a department of a general hospital in Bologna only partially equipped with an air conditioning system. To determine the environmental parameters, an ANADATA (LSI) climate analyzer with relative transducers was used. The Effective Temperature (ET), the New Effective Temperature (ET*) and the Fanger indices (PMV-PPD) were calculated using the parameters measured. Microbial count measurements were taken with an S.A.S. (Surface Air System) sampler, to ascertain the total bacterial count at 37 degrees C, and the fungal particle, Staphylococcus aureus and Pseudomonas aeruginosa counts. Carbon dioxide air concentrations were also measured to evaluate the efficacy of air exchange. The Fanger indices were not within the range of thermal comfort in most rooms (52% in winter, 62% in summer). Air microbial counts were higher in the hospital wards and surgeries than in the offices and laboratories. In particular, coagulase-positive staphylococci were present only in the air of the patients' rooms. The microbial contamination was not correlated with the air conditioning system, but probably caused by the turnover in the hospital population, the number of people and their behaviour. However the most important measure to prevent airborne contamination and to reduce the number of microorganisms in the air is an efficient source control. Better management of the air conditioning system, by means of adequate air exchange and thermal adjustment, would lead to a notable improvement in indoor air quality, especially in units with hospitalized patients.

Fourteen welders were examined with the purpose to evaluate the incidence and the type of pathologic changes of the gastrointestinal tract due to the exposure to welding fumes. The medical examination was followed by endoscopic and bioptic study of gastric and duodenal mucosa. The results show the presence of pathologic changes, mainly of flogistic type, in all the microscopic investigations, although the appearance of the mucosa was generally normal. Moreover, the results of this investigation suggest that pathologic changes of gastrointestinal tract could precede those of the respiratory tract.

The Authors have taken under study 6 office buildings and a total of 165 work stations with VDTs. Lighting conditions, postures, indoor climate and air-quality have been evaluated, besides worker's subjective approach sounded by individual questionnaire. Concerning visual comfort, it has been noticed that the most simple and well-known rules are often disregarded, such as avoiding to place a screen in front of a light source because of its glare. Also, operators postures are often incorrect, making problems related to their own fixity worse. Air conditioning systems assure almost excellent conditions for thermal comfort, but air changes are often poor and microbiologic quality suffers from plant's inadequate servicing. Finally, the survey of questionnaires shows a complaint percentage which only partly corresponds with the objective data collected, reflecting instead an uneasiness to the kind of work and its organisation.