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Global carbon storage in soils is widely discussed today because of climate uncertainty and maintaining sustainable agricultural production. Human intervention in agricultural or energy production poses many changes in soil management, which highly affects soil quality/health. Permanent grasslands fulfil a wide range of ecosystem functions and have a high potential for increasing arable land. Today, grasslands are becoming more and more intensively used, fertilized and disturbed. Optimizing their management is essential to maintain a resilient and stable ecosystem. The produced biomass is used as a forage or for energy production. We aimed at the impact of long-term grassland liming on the total content of soil organic carbon (SOC), humic substances (CHS), and microbial biomass (Cmic). Furthermore, soil reaction and available nutrient content were evaluated. Soil samples were collected from a split-plot field experiment at Mendel University in Brno (locality Kameničky). The soil was classified as Dystric Planosol Siltic, medium textured, strongly acidic, with high soil organic carbon content. The yearly liming rate was 1.4 t/ha CaO. The linkage between the soil pH, SOC, Cmic, and available nutrient content was evaluated by the multivariate exploratory techniques and regression models. Results showed that long-term liming affects both soil biota and carbon storage. [ABSTRACT FROM AUTHOR]

Poproč post-mining area in Slovakia is an old environmental burden which has been contaminated by toxic metals and semi-metals, predominantly arsenic and antimony, in connection with the extraction of antimony ores. Nowadays, revitalization work is undergoing in the closed mine with three adjacent sludge lagoons. The outcomes deal with anthropogenic manifestations of mining in-situ and their influences on microbes. Using the BIOLOG test system we identified representatives of indigenous microbes, whose adaptation to high levels of toxic metals and semi-metals are one of the main bioindicators of the state of the post-mining environment and a basic indicator of the evolution of effects on organisms of a higher ecological level. The analyses of microbial diversity in Poproč found the strain of Firmicutes to be the most abundant, with the highest diversity in the genus Bacillus. Based on the biochemical tests, the Bacillus genus was confirmed to be highly adaptable to the change in biogeochemical conditions in metalcontaminated substrates. The variable parameters of the identified representatives of the Bacillus genus correspond to its high diversity, which has been confirmed by a set of oxidative and assimilation tests. [ABSTRACT FROM AUTHOR]

This paper investigates the differences in some metabolites using Biolog FF Microplate™ system and the production of organic acids such as β-hydroxybutyric, p-hydroxyphenylacetic, and others. Another group of organic acids such as gluconic, oxalic and citric acid were studied during cultivation in a liquid medium. Four different Aspergillus niger (An) wild type strains were used as a model organism. Three strains, from the Banská Štiavnica – Šobov (An – Š), Pezinok (An – P) and Slovinky (An – Sl) localities were isolated from contaminated old mining areas with soil with ultra acidic to strong alkaline reactions. The fourth strain isolated from the Gabčíkovovo (An – G) locality was used for comparative purposes. According to the RAMP analysis, the strains are clustered into two groups, An – Š and An – P (similarity 82%), An – G and An – Sl (similarity 64%) which correlates with the pH values of the original environment. However, significant differences were found in metabolic processes in the reaction with a wide range of organic acids. In general, the reactions with D-lactic acid and D-malic acid correlate with the results of the RAMP analysis of fungal genotype similarities, the An – Š and An – P strains had an identical negative reaction, and an identical positive reaction was found in the An – Sl and An – G strains. During incubation the wild-type strains produced substantial amounts of gluconic acid, oxalic acid and small amounts of citric acid. The appearance and accumulation of organic acids was found to be highly pH dependent with the most active strain isolated from an ultra-acidic environment. The comparative strain differs entirely in the production of oxalic acid.This paper investigates the differences in some metabolites using Biolog FF Microplate™ system and the production of organic acids such as β-hydroxybutyric, p-hydroxyphenylacetic, and others. Another group of organic acids such as gluconic, oxalic and citric acid were studied during cultivation in a liquid medium. Four different Aspergillus niger (An) wild type strains were used as a model organism. Three strains, from the Banská Štiavnica – Šobov (An – Š), Pezinok (An – P) and Slovinky (An – Sl) localities were isolated from contaminated old mining areas with soil with ultra acidic to strong alkaline reactions. The fourth strain isolated from the Gabčíkovovo (An – G) locality was used for comparative purposes. According to the RAMP analysis, the strains are clustered into two groups, An – Š and An – P (similarity 82%), An – G and An – Sl (similarity 64%) which correlates with the pH values of the original environment. However, significant differences were found in metabolic processes in the reaction with a wide range of organic acids. In general, the reactions with D-lactic acid and D-malic acid correlate with the results of the RAMP analysis of fungal genotype similarities, the An – Š and An – P strains had an identical negative reaction, and an identical positive reaction was found in the An – Sl and An – G strains. During incubation the wild-type strains produced substantial amounts of gluconic acid, oxalic acid and small amounts of citric acid. The appearance and accumulation of organic acids was found to be highly pH dependent with the most active strain isolated from an ultra-acidic environment. The comparative strain differs entirely in the production of oxalic acid.This paper investigates the differences in some metabolites using Biolog FF Microplate™ system and the production of organic acids such as β-hydroxybutyric, p-hydroxyphenylacetic, and others. Another group of organic acids such as gluconic, oxalic and citric acid were studied during cultivation in a liquid medium. Four different Aspergillus niger (An) wild type strains were used as a model organism. Three strains, from the Banská Štiavnica – Šobov (An – Š), Pezinok (An – P) and Slovinky (An – Sl) localities were isolated from contaminated old mining areas with soil with ultra acidic to strong alkaline reactions. The fourth strain isolated from the Gabčíkovovo (An – G) locality was used for comparative purposes. According to the RAMP analysis, the strains are clustered into two groups, An – Š and An – P (similarity 82%), An – G and An – Sl (similarity 64%) which correlates with the pH values of the original environment. However, significant differences were found in metabolic processes in the reaction with a wide range of organic acids. In general, the reactions with D-lactic acid and D-malic acid correlate with the results of the RAMP analysis of fungal genotype similarities, the An – Š and An – P strains had an identical negative reaction, and an identical positive reaction was found in the An – Sl and An – G strains. During incubation the wild-type strains produced substantial amounts of gluconic acid, oxalic acid and small amounts of citric acid. The appearance and accumulation of organic acids was found to be highly pH dependent with the most active strain isolated from an ultra-acidic environment. The comparative strain differs entirely in the production of oxalic acid. [ABSTRACT FROM AUTHOR]