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In plants, the salicylic acid (SA) and jasmonic acid (JA) signaling pathways usually mediate the defense response to biotrophic and necrotrophic pathogens, respectively. Our previous work showed that after non-heading Chinese cabbage (NHCC) was infected with the biotrophic pathogen Hyaloperonospora parasitica, expression of the JA biosynthetic gene BcOPR3 is induced; however, its molecular mechanism remains unclear. Here, we overexpressed BcOPR3 in Arabidopsis and silenced BcOPR3 in NHCC001 plants to study the defensive role of BcOPR3 in plants against pathogen invasion. The results showed that overexpression of BcOPR3 increased the susceptibility of Arabidopsis to Pseudomonas syringae pv. tomato DC3000 ( Pst DC3000) but enhanced its resistance to Botrytis cinerea. BcOPR3-silenced NHCC001 plants with a 50% reduction in BcOPR3 expression increased their resistance to downy mildew by reducing the hyphal density and spores of H. parasitica. In addition, BcOPR3-partly silenced NHCC001 plants were also resistant to B. cinerea, which could be the result of a synergistic effect of JA and SA. These findings indicate a complicated role of BcOPR3 in the mediating defense responses to biotrophic and necrotrophic pathogens.

WRKY transcription factors play important roles in abiotic stress by directly regulating stress-related genes. However, the molecular mechanism of its involvement in salt stress in pak-choi is still poorly understood. In this study, we elucidated the function of BcWRKY1 from pak-choi (Brassica rapa ssp. chinensis) in salt stress. The expression level of BcWRKY1 showed the highest in rosette leaves among different tissues and was induced by salt and ABA treatment in pak-choi. Subcellular localization showed that BcWRKY1 was located in nucleus. The transgenic Arabidopsis overexpressing BcWRKY1 exhibited enhanced salt sensitivity and higher H

This article presents a theoretical study concerning the static and dynamic performance of an externally pressurized deep/shallow pockets hydrostatic/hydrodynamic journal-thrust floating ring bearing compensated by a flat capillary restrictor. A new fluid-lubricated hybrid bearing that can support a radial and an axial load at the same time is designed and manufactured that can satisfy modern rotary machinery at high speed. The equations governing the flow of the inner and the outer and the radial and the axial fluid film in the journal-thrust floating ring bearing together with the pressure boundary condition and the restrictor flow equation are solved by using the finite element method. The study describes the influence of the eccentricity ratio and journal rotational speed on the static and dynamic characteristics such as the load-carrying capacity, the friction moment, the friction power efficiency, the volume flow rate, the stiffness, the damping, the critical mass, etc. By analyzing the journal cent...

During the past ten years, cloud computing gradually becomes mature which is a current research hotspot. OpenStack is currently one of the most popular open source cloud platforms that developed by Rack space and NASA. It provides some toolkits to deploy cloud environment and implement cloud infrastructure like Amazon EC2. In this paper, we describe OpenStack architecture in detail and introduce its application in big data processing. This led us to the conclusion that OpenStack is a highly modular and extensible and platform and it is a good alternative if you want to hand large-scale data efficiently.

This paper presents a theoretical study and experimental method to recognize the dynamic performance (stiffness and damping coefficients) of an externally pressurized deep/shallow pockets hybrid conical bearing compensated by flat capillary restrictors. The equations governing the flow of fluid film in the conical bearing together with the pressure boundary condition and the restrictor flow equation are solved by using the finite element method. A delicate test rig is constructed and bearings having a big end diameter of 97 mm, a length of 90 mm, and a radial clearance of 0.02–0.025 mm are analyzed. It is assumed that the fluid film force of the hydrostatic/hydrodynamic conical bearing is characterized by a set of linear stiffness and damping coefficients. The experiment used the impulse excitation method to recognize these coefficients and established their characteristics under different operating conditions. Numerical results are compared with the experimental results. The stability parameters of hybrid conical, hydrodynamic, and hydrostatic bearings are compared. The results show that the hybrid conical bearing has the advantages of high load carrying capability and high stability under small eccentricity.