Search

This is the first study reporting droplet digital PCR and quantitative real time PCR for detection of Tilletia caries (syn. T. tritici), which causes common bunt of wheat and leads to yield losses of 80% in many wheat growing areas worldwide. To establish an accurate, rapid and quantifiable detection method, we tested 100 inter simple sequence repeats (ISSR) primers and obtained a species-specific fragment (515 bp) generated by ISSR 827. Then, a specific 266 bp band for the sequence characterized amplified region (SCAR) marker was produced from T. caries. The detection limit reached 50 pg/μL. Based on the SCAR marker, we further developed a higher sensitivity of quantitative real time-polymerase chain reaction (qRT-PCR) with a detection limit of 2.4 fg/μL, and droplet digital PCR (ddPCR) with a detection limit of 0.24 fg/μL. Both methods greatly improved the detection sensitivity of T. caries, which will be contribute a lot for quickly and accurately detection of T. caries, which causes wheat common bunt.

To accelerate environmentally friendly thin film photovoltaic (PV) technologies, copper-based chalcogenides are attractive as absorber materials. Chalcopyrite copper indium gallium selenide (CIGS ≡ CuIn1–xGaxSe2) is today a commercially important PV material, and it is also in many aspects a very interesting material from a scientific point of view. Copper zinc tin sulfide selenide (CZTSSe ≡ Cu2ZnSn(S1–xSex)4) is considered as an emerging alternative thin film absorber material. Ternary Cu2SnS3 (CTS) is a potential absorber material, thus its related alloys Cu2Sn1–xGexS3 (CTGS) and Cu2Sn1–xSixS3 (CTSS) are attractive due to the tunable band gap energies. CuSb(Se1–xTex)2 and CuBi(S1–xSex)2 can be potential as ultra-thin (≤ 100 nm) film absorber materials in the future. In the thesis, analyses of these Cu-based chalcogenides are based on first-principles calculations performed by means of the projector augmented wave method and the full-potential linearized augmented plane wave formalisms within the density functional theory as implemented in the VASP and WIEN2k program packages, respectively. The electronic and optical properties of CIGS (x = 0, 0.5, and 1) are studied, where the lowest conduction band (CB) and the three uppermost valence bands (VBs) are parameterized and analyzed in detail. The parameterization demonstrates that the corresponding energy dispersions of the topmost VBs are strongly anisotropic and non-parabolic even very close to the Γ-point. Moreover, the density-of-states and constant energy surfaces are calculated utilizing the parameterization, and the Fermi energy level and the carrier concentration are modeled for p-type CIGS. We conclude that the parameterization is more accurate than the commonly used parabolic approximation. The calculated dielectric function of CuIn0.5Ga0.5Se2 is also compared with measured dielectric function of CuIn0.7Ga0.3Se2 collaborating with experimentalists. We found that the overall shapes of the calculated and measured dielectric function spectra are in good agreement. The transitions in the Brillouin zone edge from the topmost and the second topmost VBs to the lowest CB are responsible for the main absorption peaks. However, also the energetically lower VBs contribute significantly to the high absorption coefficient. CTS and its related alloys are explored and investigated. For a perfectly crystalline CTS, reported experimental double absorption onset in dielectric function is for the first time confirmed by our calculations. We also found that the band gap energies of CTGS and CTSS vary almost linearly with composition over the entire range of x. Moreover, those alloys have comparable absorption coefficients with CZTSSe. Cu2XSnS4 (X = Be, Mg, Ca, Mn, Fe, Ni, and Zn) are also studied, revealing rather similar crystalline, electronic, and optical properties. Despite difficulties to avoid high concentration of anti-site pairs disordering in all compounds, the concentration is reduced in Cu2BeSnS4 partly due to larger relaxation effects. CuSb(Se1–xTex)2 and CuBi(S1–xSex)2 are suggested as alternative ultra-thin film absorber materials. Their maximum efficiencies considering the Auger effect are ~25% even when the thicknesses of the materials are between 50 and 300 nm. QC 20170523

To accelerate environmental friendly thin‐film photovoltaic technologies, earth‐abundant, non‐toxic, and low‐cost materials are demanded. We study the compounds of Cu2Sn1−xGexS3 and Cu2Sn1−xSixS3 (x = 0, 0.5, and 1) employing first‐principles method within the density functional theory. The compounds have comparable band dispersions. The band‐gap energies Eg can be tailored by cation alloying the Sn atoms with Ge or Si. The gap energies of Cu2Sn1−xGexS3 and Cu2Sn1−xSixS3, with x = 0, 0.5, and 1, vary almost linearly from 0.83 to 1.43 eV and 2.60 eV, respectively. However, the gap energy of Cu2SiS3 does not follow the linear relation for x > 0.8. The effective electron masses at the Γ‐point of the lowest conduction band are almost isotropic for all materials, which are between 0.15m0 and 0.25m0. On the other hand, the effective hole masses of the topmost valence band show very strong anisotropy for all compounds. In the (010) direction, the hole masses are estimated to be between 1.01m0 and 1.85m0, while between 0.11m0 and 0.41m0 in the (001) direction. Calculations reveal that all compounds have high absorption coefficients that are comparable with that of Cu2ZnSnS4. The absorptions in the energy region from Eg + 0.5 eV to Eg + 1.0 eV are even higher for Ge‐ and Si‐alloying of Cu2SnS3, compared with Cu2ZnSnS4. The high‐frequency dielectric constants of the compounds are between 6.8 and 8.9. Cu2Sn1−xGexS3 and Cu2Sn1−xSixS3 can be considered as potential candidates for absorber materials in thin‐film solar cells. This research has been accepted and published. © 2017 Wiley