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Tocotrienol (T3) is a subfamily of vitamin E known for its wide array of medicinal properties. This review aimed to summarize the health benefits of T3, particularly in prevention or treatment of non-communicable diseases (NCDs), including cardiovascular, musculoskeletal, metabolic, gastric, and skin disorders, as well as cancers. Studies showed that T3 could prevent various NCDs, by suppressing 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) in the mevalonate pathway, inflammatory response, oxidative stress, and alternating hormones. The efficacy of T3 in preventing/treating these NCDs is similar or greater compared to tocopherol (TF). TF may lower the efficacy of T3 because the efficacy of the combination of TF and T3 was lower than T3 alone in some studies. Data investigating the effects of T3 on osteoporosis, arthritis, and peptic ulcers in human are limited. The positive outcomes of T3 treatment obtained from the preclinical studies warrant further validation from clinical trials.

Laparoscopic ventral hernia repair incorporating a prosthetic mesh underlay, first described in 1993, has demonstrated a lower long-term recurrence rate versus open non-mesh repair. However, over the past 25 years, the laparoscopic approach to ventral/incisional hernias is utilized in only approximately 30% of cases. One of the reasons that prevents it from being utilized more often is the inability to readily, reliably, and easily close the fascial defect. A novel technique has been developed for full-thickness abdominal wall closure in laparoscopic ventral hernioplasty, utilizing puncture sites to place multiple self-locking ligature straps. Introduction of the straps into the abdominal cavity in orthogonal orientation to the skin surface, followed by subcutaneous retrieval of the contralateral tip of the strap, achieves incorporation of full-thickness abdominal wall on either side of the defect. The self-locking property of each strap allows tension to be applied in sequential fashion. Incremental tension application facilitates re-apposition of the borders in large defects. The increased width of the strap compared with conventional suture serves to resolve the force exerted upon tissue during the acute phase of defect closure. The instrumentation was tested in six ventral hernias created in resected porcine belly walls. Subsequent tests were conducted in three swine with large congenital umbilical hernias. One of the test animals was re-examined laparoscopically 30 days post repair, with full healing and no recurrence exhibited upon re-examination. We anticipate that the simplicity and functionality of this technique will translate to clinical utility in the significant cohort of human ventral hernia patients.

Sleep-disordered breathing (SDB) is associated with cardiovascular disease and systemic inflammation in adults but this remains to be explored in children, especially in children with the most common form of SDB, i.e. primary snoring/mild SDB. This pilot study investigated the relationship between the cardiovascular function and inflammation in children with mild SDB. Nineteen participants aged 5-14 years underwent overnight polysomnography, cardiac magnetic resonance imaging (aortic blood flow velocity and left and right ventricular systolic function) and assessment for inflammatory markers (intracellular cytokine analysis of T cells by flow cytometry). Parents also completed the Sleep Disturbances Scale for Children (SDSC). Children with mild SDB exhibited increased ascending aortic peak systolic velocity compared to controls (SDB 119.95 m/s vs. control 101.49 m/s, p < 0.05). No significant group differences were observed for left and right ventricular ejection fraction or mean aortic blood flow velocity from either the ascending aorta or pulmonary artery. Children with mild SDB had increased inflammatory markers as demonstrated by elevated T cell interferon gamma (IFNγ) (SDB 52 ± 4% vs. control 25 ± 3% positive cells, p < 0.005) and tumour necrosis factor alpha (TNFα) (SDB 39 ± 4% vs. control 20 ± 2% positive cells, p < 0.005) expression from CD8 + cells. A strong positive correlation was observed between ascending aorta peak blood flow velocity and both TNFα and IFNγ (TNFα, r = 0.54, p < 0.03; IFNγ, r = 0.63, p < 0.005, respectively). Polysomnography revealed that oxygen saturation (SaO 2 ) nadir was significantly lower in children with mild SDB compared to controls (SDB 92.3 ± 2.7% vs. control 94.4 ± 1.6%, p < 0.05). A lower SaO 2 nadir was associated with an increased ascending aorta peak systolic velocity (r = - 0.48, p < 0.05). As well, both a lower SaO 2 nadir and an increased ascending aorta peak systolic velocity were associated with higher SDSC Sleep-Disordered Breathing and Disorder of Initiating and Maintaining Sleep subscale scores but not the polysomnographic-derived Obstructive Apnea-Hypopnea Index. The finding of elevated ascending aortic peak systolic blood flow velocity and its association with increased inflammatory markers suggests that the profile of cardiovascular changes noted in adult SDB may also occur in children with mild SDB.

We present a fiber-coupled pump-module emitting more than 1kW of mode-stripped power at 976nm from a detachable 225µm, 0.22NA fiber. The electrical-to-optical efficiency at 1kW is ~50%. Six of these pump modules attached to a (6 + 1):1 multimode combiner enable a 5-6kW, single-mode, Yb-doped fiber amplifier.

To develop a novel endovascular chemotherapy filter (CF) able to remove excess drug from the blood during intra-arterial chemotherapy delivery (IAC), thus preventing systemic toxicities and thereby enabling higher dose IAC. A flow circuit containing 2.5 mL of ion-exchange resin was constructed. Phosphate-buffered saline (PBS) containing 50 mg doxorubicin (Dox) was placed in the flow model with the hypothesis that doxorubicin would bind rapidly to resin. To simulate IAC, 50 mg of doxorubicin was infused over 10 min into the flow model containing resin. Similar testing was repeated with porcine serum. Doxorubicin concentrations were measured over 60 min and compared to controls (without resin). Single-pass experiments were also performed. Based on these experiments, an 18F CF was constructed with resin in its tip. In a pilot porcine study, the device was deployed under fluoroscopy. A control hepatic doxorubicin IAC model (no CF placed) was developed in another animal. A second CF device was created with a resin membrane and tested in the infrarenal inferior vena cava (IVC) of a swine. In the PBS model, resin bound 76% of doxorubicin in 10 min, and 92% in 30 min (P   <   0.001). During IAC simulation, 64% of doxorubicin bound in 10 min and 96% in 60 min (P   <   0.001). On average, 51% of doxorubicin concentration was reduced during each pass in single pass studies. In porcine serum, 52% of doxorubicin bound in 10 min, and 80% in 30 min (P   <   0.05). CF device placement and administration of IAC were successful in three animals. No clot was present on the resin within the CF following the in vivo study. The infrarenal IVC swine study demonstrated promising results with up to 85% reduction in peak concentration by the CF device. An endovascular CF device was developed and shown feasible in vitro. An in vivo model was established with promising results supporting high-capacity rapid doxorubicin filtration from the blood that can be further evaluated in future studies.

Background: Endoscopic methods of saphenous vein procurement have recently been introduced. These techniques have been successful in limiting pain and wound complications, but less information on assessing potential trauma to the harvested vein segment is available., Methods: Fourteen male patients undergoing coronary artery bypass grafting were included in the study. Nine patients underwent endoscopic procurement of saphenous vein whereas 5 patients underwent procurement using standard open techniques. Histologic appearance and immunohistochemical studies (factor VIII:vWF [von Willebrand factor protein] and CD34) of the vein segments were reviewed in a blinded fashion., Results: On histologic analysis, no differences in the intima, media, or adventitia were found between endoscopically and conventionally obtained vein segments. Immunohistochemical staining for factor VIII:vWF and CD34 showed no differences between veins harvested by the two techniques., Conclusions: Endoscopic saphenous vein harvesting does not appear to traumatize the vessel wall any more than open techniques. Longitudinal assessment is necessary to evaluate long-term patency in vein grafts procured using this method.

Background: Several authors have reported success using a gas-mediated transperitoneal approach for lumbar interbody fusion. However, this approach has not been shown to reliably and predictably address segments above L4-5., Methods: The B.E.R.G. approach was attempted in 202 patients who required anterior lumbar interbody fusion (ALIF). Of those, 168 were completed successfully without conversion to an open procedure. The anterior retroperitoneal approach required no gas insufflation. The gasless environment allowed for the use of standard anterior instrumentation and a variety of fusion grafts and devices., Results: Mean hospital stay was 1.95 days, with 73% of patients discharged in <47 h following surgery. Clinical results from the first 50 patients, with a minimum 2-year follow-up, include a 92% fusion rate and 78% of patients reporting significant pain relief of greater than 50%., Conclusions: The B.E.R.G. approach offers significant technical advantages over the standard gas-mediated transperitoneal approach for ALIF. The clinical results are similar to those reported for open approaches and the gas-mediated transperitoneal approach.

A retrospective preliminary study was undertaken of combined minimally invasive instrumented lumbar fusion utilizing the BERG (balloon-assisted endoscopic retroperitoneal gasless) approach anteriorly, and a posterior small-incision approach with translaminar screw fixation and posterolateral fusion. The study aimed to quantify the clinical and radiological results using this combined technique. The traditional minimally invasive approach to the anterior lumbar spine involves gas insufflation and provides reliable access only to L5-S1 and in some cases L4-5. A gas-mediated approach yields many technical drawbacks to performing spinal surgery. A minimally invasive posterior approach involving suprafascial pedicle screw instrumentation has been developed, but without wide-spread use. Translaminar facet fixation may be a viable alternative to transpedicular fixation in a 360 degrees instrumented fusion model. Past studies have shown open 360 degrees instrumented lumbar fusion yields high arthrodesis rates. The study examined the cases of 46 patients who underwent successful 360 degrees instrumented lumbar fusion using a combined minimally invasive approach. Anterior lumbar interbody fusion (ALIF) at one or two levels was performed through the BERG approach; a gasless retroperitoneal approach to the lumbar spine allowing the use of standard anterior instrumentation. Posteriorly, all patients underwent successful decompression, translaminar fixation, and posterolateral fusion at one or two levels through one small (2.5-5.0 cm) incision. Results showed mean hospital stay of 2.02 days; mean combined blood loss was 255 cc; and mean pain relief was 56%, with 75.5% of patients reporting good, excellent, or total pain relief. Forty-two of 46 patients (93.2%) achieved a solid fusion 24 months after surgery. A total of 47% of all patients working prior to surgery returned to work following surgery. The study showed that minimally invasive 360 degrees instrumented lumbar fusion, when performed utilizing these approaches, yields a high rate of solid arthrodesis (93.3%), good pain relief, short hospital stays, low blood losses, accelerated rehabilitation, and a quick return to the workforce. The BERG approach offers technical advantages over the traditional gas-mediated laparoscopic approach to the anterior lumbar spine.

Background: Patients requiring limb bypass or coronary artery bypass procedures frequently develop vein harvest-associated complications. Minimally invasive surgical techniques that can be employed during dissection of the greater saphenous vein could potentially reduce the significant incisional morbidity concomitant with this procedure., Study Design: An endoscopic saphenous vein balloon dissector was developed and previously tested in the animal model. It was applied to a series of 16 leg bypass patients to dissect totally endoscopically the greater saphenous vein for either translocated or in situ procedures. The clinical course of these 16 patients was compared with the most recent 16 consecutive standard open saphenous vein bypass patients of similar age, disease, and risk factors., Results: No venous conduit injuries occurred with the endoscopic dissection technique, and only one minor wound seroma resulted in the harvest tunnel, requiring simple aspiration. The length of stay averaged 3.8 days, but the trend was downward to 1.8 days for the last 8 consecutive endoscopically dissected patients. In comparison, there were five major wound complications in the 16 open saphenous vein bypass patients with an average length of stay of 6.2 days., Conclusions: Total endoscopic saphenous vein dissection for either translocated or in situ leg bypass patients can be performed atraumatically for both the patient and the venous conduit. Wound/incisional complications are decreased, and length of stay appears to be reduced. Minimally invasive, endoscopic saphenous vein harvest may be beneficial for both leg bypass patients and coronary artery bypass patients.

The goal of the present study is to develop a technique for laparoscopic aortobifemoral bypass. Piglets weighing between 60 and 78 kg were anesthetized with halothane. The lateral retroperitoneal approach was preferred to the more familiar anterior transperitoneal approach and was successfully completed in 19 piglets. The piglets were placed in the right lateral decubitus position. The first port (2 cm) was inserted halfway between the tip of the 12th rib and the iliac crest. Four other trocars were placed in the retroperitoneum after balloon inflation had allowed creation of a space which permitted visualization of the aorta from the left renal artery down to the aorto-iliac junction. After evacuation of the retropneumoperitoneum, the cavity was maintained using an abdominal lift device and a retractor. Using this approach, we performed four aorto-bifemoral bypasses (end-to-end aortic anastomosis) after conventional intravenous heparinization (100 IU/kg) in less than 4 h. Blood loss did not exceed 250 ml and the hematocrit remained stable. Postmortem evaluation of the grafts revealed they were positioned as in a conventional bypass, their limbs having followed in the created retroperitoneal tunnels along the path of the native arteries. No mortality occurred before sacrifice of the animals. We believe that this first performed series of totally retroperitoneal laparoscopic aortobifemoral bypasses in the porcine model is useful in preparation for human application due to the anatomical similarities in the periaortic region.

In clinical use, the mechanical lifting technique has demonstrated the ability to displace the abdominal wall and create a useful cavity for visualization and surgical manipulation. By forming a planar ceiling, as opposed to the domed ceiling of conventional pneumoperitoneum, the abdominal organs are brought into closer proximity of the surgeon. Instrument length may be shortened, imparting greater surgical control. Without the necessity for gas sealing, entry portals are simplified. Conventional instruments (right angle clamps) may be introduced through the fan retractor insertion sites or by way of separate stab incisions. The planar lifting technique has the potential for simplifying laparoscopy and restoring instrument control to the operating surgeon.

A novel device for applying hemostatic clips in laparoscopic surgery incorporates a distal hook into a multiple-firing titanium clip applier. The hook may be used for blunt dissection of tissue, and to displace and control ducts and vessels during clip application. A single instrument may be used to achieve hemostasis in areas that are difficult to reach, and past pointing problems encountered with straight on clip appliers are alleviated. Comparative testing of the holding force of the curved clips used with this device versus the straight clips used in conventional multiple-clip appliers demonstrated a higher mean pull-off force of 0.473 lbs versus 0.33 lbs. Clinical application of the device in laparoscopic procedures including cholecystectomy, vaginal hysterectomy, Nissen fundoplication, vagotomy, varicocelectomy, and lymphadenectomy show the utility of the hook clip applier.

The evolution of a preferred technique for laparoscopic inguinal hernia repair has been occurring over the past several years. The early work of Ger involved a stapled closure of the dilated internal ring using a specialized 12-mm. instrument, which combined the functions of tissue approximation and stapling. This was followed by a prosthetic mesh plug technique of Schultz and Corbitt, which consisted of a free mesh plug occlusion of the inguinal canal, combined with prosthetic patch coverage of the hernia defect.

A fan retractor and a mechanical lifting arm were developed to achieve planar displacement of the anterior abdominal wall for gasless laparoscopic procedures. The technique permits the use of conventional open surgical instruments as well as laparoscopic tools through minilaparotomy incisions. It also potentially addresses the technical constraints imposed by pneumoperitoneum, and physiologic concerns regarding carbon dioxide insufflation. Gynecologic, general surgical, and trauma procedures were performed in 104 patients, with successful completion of 86.5%. Continued application is necessary to delineate the full range of benefits of laparoscopy without insufflation.

We report a quantitative determination of the relative contribution of various factors leading to arterial lumen enlargement during transluminal angioplasty. Mechanical tests were conducted on both normal and atherosclerotic artery necropsy specimens. In the range of dilating pressures tested (0 to 3.4 atm or 0 to 50 lb/in2), content extrusion of fluid from the plaque accounted for 6% to 12% of the overall lumen area increase, while compaction of the plaque accounted for only 1% to 1.5%. Most of the increase (86.8% to 93%) was due to plaque and arterial wall disruption. The mechanism of disruption involved shearing of the plaque from the underlying artery at relatively low dilating pressures, followed by longitudinal tearing in the arterial wall at higher pressures. Diseased arteries were observed to dilate significantly more than nondiseased arteries at dilating pressures greater than or equal to 1.36 atm or 20 lb/in2 (P less than 0.05). In the range of stenoses tested (10% to 50% based on diameter reduction), the mean dilating pressure required to increase the lumen cross-sectional area by 50% was approximately 1.5 atm or 22 lb/in2. Based on these studies, we conclude that balloon dilatation results arise mainly from plaque and arterial wall disruption.

The shear force gauge is a device that will allow surgeons to develop a sense for the amount of shear force exerted on the arterial endothelium during balloon embolectomy. As a teaching device, hopefully it will decrease the number of shear force related complications connected to the use of the balloon embolectomy catheter.

We quantitatively determined the relative contribution of various factors leading to arterial lumen enlargement during transluminal angioplasty. Mechanical tests were conducted on both normal and atherosclerotic artery necropsy specimens. In our range of dilating pressures (0-3.4 atm or 0-50 lb/in2), content extrusion of fluid from the plaque accounted for 6-12% of the overall lumen area increase, while compaction of the plaque accounted for only 1-1.5%. The majority of the increase, 86.8-93%, was due to plaque and arterial wall disruption. The mechanism of disruption began with shearing of the plaque from the underlying artery at relatively low dilating pressures and continued with longitudinal tearing and stretching of the arterial wall at higher pressures. Diseased arteries dilated significantly more than nondiseased arteries at dilating pressures greater than or equal to 1.36 atm or 20 lb/in2 (P less than .05). In the range of stenoses that were tested (10-50%), the mean dilating pressure required to increase the lumen cross-sectional area by 50% was approximately 1.5 atm or 22 lb/in2.

An improved technique for internal mammary artery graft preparation is described. Following cautery dissection of the internal mammary artery (IMA) pedicle, the pedicle investing fascia is incised to the adventitial level along a single plane. This incision allows the tissue around the internal mammary artery to fall away and severs the muscular constrictions that often surround the internal mammary artery. Balloon calibration is performed to identify remaining constrictions and to relieve internal mammary artery spasm. A shear force limiting gauge insures that the exerted balloon force remains below the level demonstrated to cause intimal damage during electron microscopic studies. This technique allows full internal mammary artery distention without the devascularizing effects of full skeletonization. Distention of the distal internal mammary artery provides an enlarged hood to facilitate suture placement. Elongation of the internal mammary artery during balloon calibration aids in the performance of sequential grafts. This technique has been applied to 793 patients over the past five years. Postcalibration flow rates increased 3- to 18-fold over precalibration flow rates. Two early occlusions occurred during this series, one due to endothelial strippage prior to the development and use of the shear force limiting gauge. Follow-up showed 93.3% of patients to be asymptomatic. This combination of fascial incision and balloon calibration appears to offer safe technical and functional improvements to the performance of the internal mammary artery graft.

Our experience with combined balloon catheter thrombectomy and balloon dilation for the treatment of acute thrombosis is reported. Eighteen patients underwent the combined procedures between 1981 and 1988. Primary thrombectomy and balloon dilation were performed in 14 patients, and additional reconstruction was performed in three patients. The superficial femoral artery was the site of thrombectomy and dilation in 13 patients, and the external iliac artery was the site in the remaining five patients. Successful dilation was accomplished in all patients, with stenotic sites reduced below 30% by angiography, and ankle/brachial indexes increased by 0.15 or more. No operative deaths or complications occurred. Follow-up of superficial femoral artery dilations showed a 90% patency rate continued out to 4 years and a 40% patency rate at 5 years. Combined thrombectomy and interventional catheter therapy may aid in the care of this difficult to treat vascular patient group.

Arterial embolism is usually caused by cardiac disease, and atherosclerotic coronary vascular disease is the primary precursor. Other cardiac states, as well as several uncommon causes, are part of the etiologic spectrum. The earliest signs are pain, paresthesias, pallor, and pulselessness. Severe ischemia is indicated by paralysis, a late feature. Arterial embolism and acute thrombosis can be difficult to distinguish, and deep venous thrombosis may also be suspected in the differential diagnosis. To restore arterial flow, anticoagulation treatment with heparin (Lipo-Hepin, Liquaemin) is given and surgical embolectomy is performed. Heparin infusion is continued until the patient is ambulatory, and then warfarin sodium (Coumadin, Panwarfin) is given over the long term. Fibrinolysis has also been used to treat acute arterial occlusion. Complications of embolism must be carefully guarded against, and additional procedures are sometimes necessary.

The inability to successfully position angioplasty catheters and the occurrence of complications during angioplasty procedures can, in part, be related to the shear forces generated during catheter introduction. Shear forces are the axial contact forces that the catheter system exerts on the inner arterial surfaces during advancement. The shear forces exerted by three different catheter designs (coaxial dilator, coaxial balloon, and linear extrusion) were measured in normal and atherosclerotic arteries; in modeled stenoses of variable severity, length, and compliance; and in modeled vessel angulations. The results with modeled vessels show that the linear extrusion catheter reduces the level of shear forces, particularly in narrow, long, noncompliant stenoses and in tortuous vessels. The stenotic artery results also show that the linear extrusion catheter minimizes these forces in tight lesions. The relative differences in forces are explained by the mechanism of action for each catheter. The reported occurrences of technical difficulties, complications, and long-term patency rates are then interpreted on the basis of the relative differences in measured shear forces. The results of this study combined with preliminary clinical data indicate that linear extrusion should facilitate placement and reduce associated complications.

Potential differences in flow rates between reversed and in situ saphenous vein bypass grafts were evaluated. One hundred ten greater saphenous vein segments containing isolated valves were examined with fiber-optic angioscopy during pulsatile and nonpulsatile flow. Valve competency was determined, and the degree of luminal obstruction caused by the valve during reversed flow was calculated with caliper measurements of the video image. Flow measurements were obtained before and after valvulotomy, in reversed and nonreversed vein orientations. Increased flow rates occurred during pulsatile irrigation only, after valvulotomy in vein segments with diameters less than 2.5 mm (p less than 0.001, Bonferroni t test). In these small-diameter vein segments, the flow rate in reversed valve-intact vein was 94.4 +/- 28.9 ml/min (mean +/- 1 standard deviation), the flow rate in reversed valve-disrupted vein was 136.4 +/- 36.5 ml/min, and the flow rate in nonreversed valve-disrupted vein was 137.8 +/- 31.3 ml/min. In 22 vein segments, luminal obstruction caused by the intact valve was measured angioscopically. A small valve orifice was found to be related to a large increase in flow rate after valvulotomy (p less than 0.02, least-squares regression). In addition, veins with diameters less than 2.5 mm have significantly smaller valve orifices compared with veins with diameters greater than 2.5 mm. These results present important clinical implications as the number of distal extremity reconstructions increases.

A review was conducted of 61 patients who underwent intraoperative balloon dilatation over the past five years. Of the 62 dilatations in this patient group, 80% were performed in conjunction with a reconstructive procedure, and 20% were performed as a primary procedure. Dilatations were performed with the linear extrusion balloon catheter. A 94% rate of followup was achieved, with a mean followup of 16.3 months (range 1-60 months). Life table analysis reveals a 95% initial success rate decreasing to an 81% patency rate by the end of the fifth year for iliac dilatations, a 97% initial and 58% five-year patency rate for superficial femoral dilatations, and an 86% initial and 37% five-year patency rate for popliteal dilatations. 21.3% of the patients died during the followup period. No deaths occurred as a result of intraoperative angioplasty. One arterial rupture occurred, requiring a bypass graft at the same surgery. We conclude that intraoperative balloon dilatation in an adjunctive setting may offer clinical benefits to vascular patients while requiring little additional operative time.