1. YjgF is required for isoleucine biosynthesis when Salmonella enterica is grown on pyruvate medium
- Author
-
Christopherson, Melissa R., Schmitz, G.E., and Downs, Diana M.
- Subjects
Salmonella -- Physiological aspects ,Isoleucine -- Properties ,Biosynthesis -- Research ,Pyruvates -- Properties ,Pyruvates -- Influence ,Proteins -- Properties ,Proteins -- Influence ,Biological sciences - Abstract
The YjgF/YER057c/UK114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. In Salmonella enterica, a deletion of yjgF results in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. Feedback inhibition of IlvA is required for the curative effect of isoleucine on glucose-serine medium. On pyruvate medium, yjgF mutants are unable to synthesize enough isoleucine for growth. From this study, we conclude that the isoleucine requirement of a yjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants.
- Published
- 2008