Search

Your search keyword '"Dai, Yang-Xue"' showing total 12 results
Start Over Author "Dai, Yang-Xue" Publication Year Range Last 50 years
12 results on '"Dai, Yang-Xue"'

12. Endogenous Bos taurusRECQL is predominantly monomeric and more active than oligomers

Author

Liu, Na-Nv, Song, Ze-Yu, Guo, Hai-Lei, Yin, Hu, Chen, Wei-Fei, Dai, Yang-Xue, Xin, Ben-Ge, Ai, Xia, Ji, Lei, Wang, Qing-Man, Hou, Xi-Miao, Dou, Shuo-Xing, Rety, Stephane, and Xi, Xu-Guang

Abstract

There is broad consensus that RecQ family helicase is a high-order oligomer that dissociates into a dimer upon ATP binding. This conclusion is based mainly on studies of highly purified recombinant proteins, and the oligomeric states of RecQ helicases in living cells remain unknown. We show here that, in contrast to current models, monomeric RECQL helicase is more abundant than oligomer/dimer forms in living cells. Further characterization of endogenous BtRECQL and isolated monomeric BtRECQL using various approaches demonstrates that both endogenous and recombinant monomeric BtRECQL effectively function as monomers, displaying higher helicase and ATPase activities than dimers and oligomers. Furthermore, monomeric BtRECQL unfolds intramolecular G-quadruplex DNA as efficiently as human RECQL and BLM helicases. These discoveries have implications for understanding endogenous RECQL oligomeric structures and their regulation. It is worth revisiting oligomeric states of the other members of the RecQ family helicases in living cells.

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results