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Your search keyword '"Dailey, Jennifer"' showing total 34 results
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34 results on '"Dailey, Jennifer"'

1. Detecting Lyme Disease Using Antibody-Functionalized Single-Walled Carbon Nanotube Transistors

Author

Lerner, Mitchell B., Dailey, Jennifer, Goldsmith, Brett R., Brisson, Dustin, and Johnson, A. T. Charlie

Subjects
Physics - Biological Physics, Condensed Matter - Materials Science, Quantitative Biology - Biomolecules
Abstract

We examined the potential of antibody-functionalized single-walled carbon nanotube (SWNT) field-effect transistors (FETs) for use as a fast and accurate sensor for a Lyme disease antigen. Biosensors were fabricated on oxidized silicon wafers using chemical vapor deposition grown carbon nanotubes that were functionalized using diazonium salts. Attachment of Borrelia burgdorferi (Lyme) flagellar antibodies to the nanotubes was verified by Atomic Force Microscopy and electronic measurements. A reproducible shift in the turn-off voltage of the semiconducting SWNT FETs was seen upon incubation with Borrelia burgdorferi flagellar antigen, indicative of the nanotube FET being locally gated by the residues of flagellar protein bound to the antibody. This sensor effectively detected antigen in buffer at concentrations as low as 1 ng/ml, and the response varied strongly over a concentration range coinciding with levels of clinical interest. Generalizable binding chemistry gives this biosensing platform the potential to be expanded to monitor other relevant antigens, enabling a multiple vector sensor for Lyme disease. The speed and sensitivity of this biosensor make it an ideal candidate for development as a medical diagnostic test., Comment: 10 pages, 3 figures, appears in Biosensors and Bioelectronics

Published
2013
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2. Hybrids of a Genetically Engineered Antibody and a Carbon Nanotube Transistor for Detection of Prostate Cancer Biomarkers

Author

Lerner, Mitchell B., DSouza, Jimson, Pazina, Tatiana, Dailey, Jennifer, Goldsmith, Brett R., Robinson, Matthew K., and Johnson, A. T. Charlie

Subjects
Physics - Biological Physics, Condensed Matter - Materials Science, Quantitative Biology - Biomolecules
Abstract

We developed a novel detection method for osteopontin (OPN), a new biomarker for prostate cancer, by attaching a genetically engineered single chain variable fragment (scFv) protein with high binding affinity for OPN to a carbon nanotube field-effect transistor (NTFET). Chemical functionalization using diazonium salts is used to covalently attach scFv to NT-FETs, as confirmed by atomic force microscopy, while preserving the activity of the biological binding site for OPN. Electron transport measurements indicate that functionalized NT-FET may be used to detect the binding of OPN to the complementary scFv protein. A concentration-dependent increase in the source-drain current is observed in the regime of clinical significance, with a detection limit of approximately 30 fM. The scFv-NT hybrid devices exhibit selectivity for OPN over other control proteins. These devices respond to the presence of OPN in a background of concentrated bovine serum albumin, without loss of signal. Based on these observations, the detection mechanism is attributed to changes in scattering at scFv protein-occupied defect sites on the carbon nanotube sidewall. The functionalization procedure described here is expected to be generalizable to any antibody containing an accessible amine group, and to result in biosensors appropriate for detection of corresponding complementary proteins at fM concentrations., Comment: 11 pages, 5 figures, appears in ACS Nano

Published
2013
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3. A Carbon Nanotube Immunosensor for Salmonella

Author

Lerner, Mitchell, Goldsmith, Brett, McMillon, Ronald, Dailey, Jennifer, Pillai, Shreekumar, Singh, Shree R., and Johnson, A. T. Charlie

Subjects
Condensed Matter - Materials Science
Abstract

Antibody-functionalized carbon nanotube devices have been suggested for use as bacterial detectors for monitoring of food purity in transit from the farm to the kitchen. Here we report progress towards that goal by demonstrating specific detection of Salmonella in complex nutrient broth solutions using nanotube transistors functionalized with covalently-bound anti-Salmonella antibodies. The small size of the active device region makes them compatible with integration in large-scale arrays. We find that the on-state current of the transistor is sensitive specifically to the Salmonella concentration and saturates at low concentration (<1000 cfu/ml). In contrast, the carrier mobility is affected comparably by Salmonella and other bacteria types, with no sign of saturation even at much larger concentrations (10^8 cfu/ml)., Comment: 8 pages, 3 figures, 1 table, appears in AIP Advances

Published
2013
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4. List of contributors

Author

Ashcroft, Christopher M., primary, Bodart-Le Guen, Come, additional, Campana, Filippo, additional, Cicoira, Fabio, additional, Cole, Jacqueline M., additional, Dailey, Jennifer, additional, Deibel, Carsten, additional, Ehrenfreund, Eitan, additional, Frohna, Kyle, additional, Fusella, Michael A., additional, Gao, Jun, additional, Gather, Malte C., additional, Goetz, Katelyn P., additional, Graf, Arko, additional, Günter, Peter, additional, Haase, Katherina, additional, Hambsch, Mike, additional, Hase, Hannes, additional, Hasegawa, Tatsuo, additional, Heiber, Michael C., additional, Hofmann, Anna I., additional, Hu, Shiyu, additional, Jang, Hyun-June, additional, Jazbinsek, Mojca, additional, Jurchescu, Oana D., additional, Kageyama, Hiroshi, additional, Katz, Howard E., additional, Keum, Changmin, additional, Kitto, Tom, additional, Kroon, Renee, additional, Kühn, Oliver, additional, Li, Hui, additional, Lin, YunHui L., additional, Lüssem, Björn, additional, Mannsfeld, Stefan C.B., additional, Marrocchi, Assunta, additional, Mayer, Kathryn, additional, Müller, Christian, additional, Nguyen, Tho Duc, additional, O’Carroll, Deirdre M., additional, Petty, Michael C., additional, Rand, Barry P., additional, Reineke, Sebastian, additional, Rossetti, Nicolo, additional, Salzmann, Ingo, additional, Savikhin, Victoria, additional, Sciosci, Daniele, additional, Sharapov, Valerii, additional, Sheleg, Gil, additional, Shi, Wei, additional, Shirota, Yasuhiko, additional, Song, Jian, additional, Stranks, Samuel D., additional, Teixeira da Rocha, Cecilia, additional, Tessler, Nir, additional, Toney, Michael F., additional, Trombettoni, Valeria, additional, Tropf, Laura, additional, Turak, Ayse, additional, Vaccaro, Luigi, additional, Vardeny, Zeev Valy, additional, Wagenpfahl, Alexander, additional, Will, Paul-Anton, additional, Willets, Katherine, additional, Yu, Junsheng, additional, Zaumseil, Jana, additional, and Zessin, Jakob, additional

Published
2019
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8. Beta-scission of tertiary alkyl hypochlorites promoted by phase-transfer catalysis

Author

Dailey, Jennifer I., Hays, Ryan S., Lee, Hua, Mitchell, Randall M., Ries, Jennifer J., Landolt, Robert G., Husmann, Hollie H., Lockridge, Joseph b., and Hendrickson, William H.

Subjects
Organic compounds -- Research, Chemical reactions -- Analysis, Alkylates -- Research, Catalysis -- Analysis, Biological sciences, Chemistry
Abstract

Two-phase reactions of tertiary alcohols with aqueous sodium hypochlorite at approximately 9 pH are examined to delineate the scope of the phase-transfer catalyst tetrabutylammonium hydrogen sulfate mediated beta-scissions and the fate of the alkyl groups cleaved. Data indicate that the reaction yields beta-scission ketone products.

Published
2000

22. CD14++CD16+ Monocytes Are Enriched by Glucocorticoid Treatment and Are Functionally Attenuated in Driving Effector T Cell Responses

Author

Liu, Baoying, primary, Dhanda, Ashwin, additional, Hirani, Sima, additional, Williams, Emily L., additional, Sen, H. Nida, additional, Martinez Estrada, Fernando, additional, Ling, Diamond, additional, Thompson, Ian, additional, Casady, Megan, additional, Li, Zhiyu, additional, Si, Han, additional, Tucker, William, additional, Wei, Lai, additional, Jawad, Shayma, additional, Sura, Amol, additional, Dailey, Jennifer, additional, Hannes, Susan, additional, Chen, Ping, additional, Chien, Jason L., additional, Gordon, Siamon, additional, Lee, Richard W. J., additional, and Nussenblatt, Robert B., additional

Published
2015
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26. Sensitive and Selective NO2Sensing Based on Alkyl- and Alkylthio-Thiophene Polymer Conductance and Conductance Ratio Changes from Differential Chemical Doping

Author

Li, Hui, Dailey, Jennifer, Kale, Tejaswini, Besar, Kalpana, Koehler, Kirsten, and Katz, Howard E.

Abstract

NO2-responsive polymer-based organic field-effect transistors (OFETs) are described, and room-temperature detection with high sensitivity entirely from the semiconductor was achieved. Two thiophene polymers, poly(bisdodecylquaterthiophene) and poly(bisdodecylthioquaterthiophene) (PQT12 and PQTS12, respectively), were used as active layers to detect a concentration at least as low as 1 ppm of NO2. The proportional on-current change of OFETs using these polymers reached over 400% for PQTS12, which is among the highest sensitivities reported for a NO2-responsive device based on an organic semiconducting film. From measurements of cyclic voltammetry and the electronic characteristics, we found that the introduction of sulfurs into the side chains induces traps in films of the PQTS12 and also decreases domain sizes, both of which could contribute to the higher sensitivity of PQTS12 to NO2gas compared with PQT12. The ratio of responses of PQTS12 and PQT12 is higher for exposures to lower concentrations, making this parameter a means of distinguishing responses to low concentrations for extended times from exposures to high concentrations from shorter times. The responses to nonoxidizing vapors were much lower, indicating good selectivity to NO2of two polymers. This work demonstrates the capability of increasing selectivity and calibration of OFET sensors by modulating redox and aggregation properties of polymer semiconductors.

Published
2017
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27. A carbon nanotube immunosensor for Salmonella

Author

Lerner, Mitchell B., primary, Goldsmith, Brett R., additional, McMillon, Ronald, additional, Dailey, Jennifer, additional, Pillai, Shreekumar, additional, Singh, Shree R., additional, and Johnson, A. T. Charlie, additional

Published
2011
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32. CD14++CD16+ Monocytes Are Enriched by Glucocorticoid Treatment and Are Functionally Attenuated in Driving Effector T Cell Responses.

Author

Baoying Liu, Dhanda, Ashwin, Hirani, Sima, Williams, Emily L., Sen, H. Nida, Martinez Estrada, Fernando, Ling, Diamond, Thompson, Ian, Casady, Megan, Zhiyu Li, Han Si, Tucker, William, Lai Wei, Jawad, Shayma, Sura, Amol, Dailey, Jennifer, Hannes, Susan, Ping Chen, Chien, Jason L., and Gordon, Siamon

Subjects
*MONOCYTES, *CD14 antigen, *AUTOIMMUNITY, *CELL membranes, *GENE expression, *CELL proliferation, *GLUCOCORTICOIDS, *AUTOIMMUNE diseases
Abstract

Human peripheral monocytes have been categorized into three subsets based on differential expression levels of CD14 and CD16. However, the factors that influence the distribution of monocyte subsets and the roles that each subset plays in autoimmunity are not well studied. In this study, we show that circulating monocytes from patients with autoimmune uveitis exhibit a skewed phenotype toward intermediate CD14++CD16+ cells, and that this is associated with glucocorticoid therapy. We further demonstrate that CD14++ CD16+ monocytes from patients and healthy control donors share a similar cell-surface marker and gene expression profile. Comparison of the effects of intermediate CD14++CD16+ monocytes with classical CD14++CD16- and nonclassical CD14+CD16++ monocytes revealed that the intermediate CD14++CD16+ subset had an attenuated capacity to promote both naive CD4+ T cell proliferation and polarization into a Th1 phenotype, and memory CD4+ T cell proliferation and IL-17 expression. Furthermore, CD14++CD16+ cells inhibit CD4+ T cell proliferation induced by other monocyte subsets and enhance CD4+ T regulatory cell IL-10 expression. These data demonstrate the impact of glucocorticoids on monocyte phenotype in the context of autoimmune disease and the differential effects of monocyte subsets on effector T cell responses. [ABSTRACT FROM AUTHOR]

Published
2015
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33. Your Favorite Trees.

Author

Wright, T., Barnett, Britney, Pittman, Jay, Reber, Shane, Zureich, Deb, Sorrell, Jeannie, DiModica, Joseph, and Dailey, Jennifer

Subjects
*TREES, *FORESTRY periodicals
Abstract

The article discusses the favorite trees of the readers of the journal related to forestry in the U.S. Britney Barnett appreciates and prefers the Ponderosa Pine because of its uniqueness. Shane Reber likes American Sycamore because it can be smelled before it is seen. Deb Zureich prefers the giant red oak because it is a symbol of strength.

Published
2015

34. Sensitive and Selective NO 2 Sensing Based on Alkyl- and Alkylthio-Thiophene Polymer Conductance and Conductance Ratio Changes from Differential Chemical Doping.

Author

Li H, Dailey J, Kale T, Besar K, Koehler K, and Katz HE

Abstract

NO 2 -responsive polymer-based organic field-effect transistors (OFETs) are described, and room-temperature detection with high sensitivity entirely from the semiconductor was achieved. Two thiophene polymers, poly(bisdodecylquaterthiophene) and poly(bisdodecylthioquaterthiophene) (PQT12 and PQTS12, respectively), were used as active layers to detect a concentration at least as low as 1 ppm of NO 2 . The proportional on-current change of OFETs using these polymers reached over 400% for PQTS12, which is among the highest sensitivities reported for a NO 2 -responsive device based on an organic semiconducting film. From measurements of cyclic voltammetry and the electronic characteristics, we found that the introduction of sulfurs into the side chains induces traps in films of the PQTS12 and also decreases domain sizes, both of which could contribute to the higher sensitivity of PQTS12 to NO 2 gas compared with PQT12. The ratio of responses of PQTS12 and PQT12 is higher for exposures to lower concentrations, making this parameter a means of distinguishing responses to low concentrations for extended times from exposures to high concentrations from shorter times. The responses to nonoxidizing vapors were much lower, indicating good selectivity to NO 2 of two polymers. This work demonstrates the capability of increasing selectivity and calibration of OFET sensors by modulating redox and aggregation properties of polymer semiconductors.

Published
2017
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