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37 results on '"Gallardo, Cesar"'

3. Classification of Polyethylene Terephthalate Bottles in a Recycling Plant

Author

Godoy, Diego Alberto, Albornoz, Enrique Marcelo, Selva, Ricardo, Ibarra, Nicolas, Gallardo, Cesar, Filipe, Joaquim, Editorial Board Member, Ghosh, Ashish, Editorial Board Member, Prates, Raquel Oliveira, Editorial Board Member, Zhou, Lizhu, Editorial Board Member, Nesmachnow, Sergio, editor, and Hernández Callejo, Luis, editor

Published
2023
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5. Shigella IpaA mediates actin bundling through diffusible vinculin oligomers with activation imprint

Author

Valencia-Gallardo, Cesar, Aguilar-Salvador, Daniel-Isui, Khakzad, Hamed, Cocom-Chan, Benjamin, Bou-Nader, Charles, Velours, Christophe, Zarrouk, Yosra, Le Clainche, Christophe, Malosse, Christian, Lima, Diogo Borges, Quenech’Du, Nicole, Mazhar, Bilal, Essid, Sami, Fontecave, Marc, Asnacios, Atef, Chamot-Rooke, Julia, Malmström, Lars, and Tran Van Nhieu, Guy

Published
2023
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14. ShigellaipaA mediates actin bundling through diffusible vinculin oligomers with activation imprint

Author

Valencia-Gallardo, Cesar, primary, Aguilar-Salvador, Daniel-Isui, additional, Khakzad, Hamed, additional, Cocom-Chan, Benjamin, additional, Bou-Nader, Charles, additional, Velours, Christophe, additional, Zarrouk, Yosra, additional, Le Clainche, Christophe, additional, Malosse, Christian, additional, Lima, Diogo Borges, additional, Quenech’Du, Nicole, additional, Mazhar, Bilal, additional, Essid, Sami, additional, Fontecave, Marc, additional, Asnacios, Atef, additional, Chamot-Rooke, Julia, additional, Malmström, Lars, additional, and Van Nhieu, Guy Tran, additional

Published
2022
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19. Shigella ipaA Mediates Actin Bundling Through Diffusible Vinculin Oligomers With Activation Imprint

Author

Aguilar-Salvador, Daniel-Isui, primary, Valencia-Gallardo, Cesar, additional, Khakzad, Hamed, additional, Bou-Nader, Charles, additional, Le Clainche, Christophe, additional, Malosse, Christian, additional, Borges Lima, Diogo, additional, Cocom-Chan, Benjamin, additional, Quenech’Du, Nicole, additional, Mazhar, Bilal, additional, Essid, Sami, additional, Fattaccioli, Jacques, additional, Fontecave, Marc, additional, Asnacios, Atef, additional, Chamot-Rooke, Julia, additional, Malmström, Lars, additional, and Tran Van Nhieu, Guy, additional

Published
2021
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20. Vinculin targeting by Shigella IpaA promotes stable cell adhesion independent of mechanotransduction

Author

Valencia-Gallardo, Cesar, Aguilar-Salvador, Daniel-Isui, Khakzad, Hamed, Bou-Nader, Charles, Malosse, Christian, Lima, Diogo Borges, Bello, Chakir, Cocom-Chan, Benjamin, Quenech’Du, Nicole, Mazhar, Bilal, Javelaud, Delphine, Fattaccioli, Jacques, Mauviel, Alain, Fontecave, Marc, Asnacios, Atef, Chamot-Rooke, Julia, Malmström, Lars, and Van Nhieu, Guy Tran

Subjects
macromolecular substances
Abstract

Shigella , the causative agent of bacillary dysentery, invades epithelial cells by injecting type III effectors that locally reorganize the actin cytoskeleton 1 . The type III effector IpaA targets the focal adhesion protein vinculin to induce bacterial adhesion associated with the recruitment of mature focal adhesion markers, despite the inability of bacteria to exert significant counter-forces 2, 3 . Here, we show that three vinculin-binding sites (VBSs) exposed at the IpaA C-terminus act in a cooperative manner to trigger a yet unreported mode of vinculin activation through specific interactions with sites in the vinculin head sub-domain D2. Structural modeling based on the mass spectrometry identification of interacting residues by cross-linking indicated that upon IpaA binding to vinculin D2, vinculin head sub-domains undergo major conformational changes leading to higher order heterocomplexes and vinculin homo-trimers. IpaA-mediated vinculin activation induces the formation of large and stable focal adhesions resisting the action of actin relaxing drugs. This property enables IpaA to rapidly elicit strong cell adhesion to fibronectin-coated substrates. While Shigella IpaA promotes strong adhesion in the absence of mechanotransduction, its mode of vinculin activation likely reflects a key step during maturation of cell adhesions driven by acto-myosin contractility.

Published
2019
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21. ipaA triggers vinculin oligomerization to strengthen cell adhesion during Shigella invasion

Author

Valencia-Gallardo, Cesar, primary, Aguilar-Salvador, Daniel-Isui, additional, Khakzad, Hamed, additional, Bou-Nader, Charles, additional, Malosse, Christian, additional, Lima, Diogo Borges, additional, Bello, Chakir, additional, Cocom-Chan, Benjamin, additional, Quenech’Du, Nicole, additional, Mazhar, Bilal, additional, Javelaud, Delphine, additional, Fattaccioli, Jacques, additional, Mauviel, Alain, additional, Fontecave, Marc, additional, Asnacios, Atef, additional, Chamot-Rooke, Julia, additional, Malmström, Lars, additional, and Van Nhieu, Guy Tran, additional

Published
2019
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22. Shigella IpaA Binding to Talin Stimulates Filopodial Capture and Cell Adhesion

Author

Valencia-Gallardo, Cesar, Bou-Nader, Charles, Aguilar-Salvador, Daniel-Isui, Carayol, Nathalie, Quenech’Du, Nicole, Pecqueur, Ludovic, Park, HaJeung, Fontecave, Marc, Izard, Tina, Tran Van Nhieu, Guy, Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie des Processus Biologiques (LCPB), Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Chaire Chimie des processus biologiques, and Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

27. Shubayqa 6 : a new Late Natufian and Pre-Pottery Neolithic A settlement in north-east Jordan

Author

Arranz Otaegui, Amaia, Boaretto, Elisabetta, Bocaege, Emmy, Estrup, Erin, Martinez-Gallardo, Cesar, Pantos, Alexis, University of Copenhagen = Københavns Universitet (KU), Weizmann Institute of Science [Rehovot, Israël], De la Préhistoire à l'Actuel : Culture, Environnement et Anthropologie (PACEA), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Chercheur indépendant, and Glasgow School of Art

Subjects
[SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, Neolithic, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

28. The Shigella type III effector IpgD recodes Ca 2+ signals during invasion of epithelial cells

Author

Sun, Chun Hui, primary, Wacquier, Benjamin, additional, Aguilar, Daniel I, additional, Carayol, Nathalie, additional, Denis, Kevin, additional, Boucherie, Sylviane, additional, Valencia‐Gallardo, Cesar, additional, Simsek, Ceren, additional, Erneux, Christophe, additional, Lehman, Alexandre, additional, Enninga, Jost, additional, Arbibe, Laurence, additional, Sansonetti, Philippe, additional, Dupont, Geneviève, additional, Combettes, Laurent, additional, and Tran Van Nhieu, Guy, additional

Published
2017
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30. The Shigella type III effector IpgD recodes Ca2+ signals during invasion of epithelial cells.

Author

Sun, Chun Hui, Wacquier, Benjamin, Aguilar, Daniel I, Carayol, Nathalie, Denis, Kevin, Boucherie, Sylviane, Valencia‐Gallardo, Cesar, Simsek, Ceren, Erneux, Christophe, Lehman, Alexandre, Enninga, Jost, Arbibe, Laurence, Sansonetti, Philippe, Dupont, Geneviève, Combettes, Laurent, and Tran Van Nhieu, Guy

Subjects
SHIGELLA, EPITHELIAL cells, CALPAIN genetics, PROTEINASE genetics, PATHOGENIC microorganisms
Abstract

The role of second messengers in the diversion of cellular processes by pathogens remains poorly studied despite their importance. Among these, Ca2+ virtually regulates all known cell processes, including cytoskeletal reorganization, inflammation, or cell death pathways. Under physiological conditions, cytosolic Ca2+ increases are transient and oscillatory, defining the so-called Ca2+ code that links cell responses to specific Ca2+ oscillatory patterns. During cell invasion, Shigella induces atypical local and global Ca2+ signals. Here, we show that by hydrolyzing phosphatidylinositol-(4,5)bisphosphate, the Shigella type III effector IpgD dampens inositol-(1,4,5)trisphosphate (InsP3) levels. By modifying InsP3 dynamics and diffusion, IpgD favors the elicitation of long-lasting local Ca2+ signals at Shigella invasion sites and converts Shigella-induced global oscillatory responses into erratic responses with atypical dynamics and amplitude. Furthermore, IpgD eventually inhibits InsP3-dependent responses during prolonged infection kinetics. IpgD thus acts as a pathogen regulator of the Ca2+ code implicated in a versatility of cell functions. Consistent with this function, IpgD prevents the Ca2+-dependent activation of calpain, thereby preserving the integrity of cell adhesion structures during the early stages of infection. [ABSTRACT FROM AUTHOR]

Published
2017
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32. ShigellaIpaA mediates actin bundling through diffusible vinculin oligomers with activation imprint

Author

Valencia-Gallardo, Cesar, Aguilar-Salvador, Daniel-Isui, Khakzad, Hamed, Cocom-Chan, Benjamin, Bou-Nader, Charles, Velours, Christophe, Zarrouk, Yosra, Le Clainche, Christophe, Malosse, Christian, Lima, Diogo Borges, Quenech’Du, Nicole, Mazhar, Bilal, Essid, Sami, Fontecave, Marc, Asnacios, Atef, Chamot-Rooke, Julia, Malmström, Lars, and Tran Van Nhieu, Guy

Abstract

Upon activation, vinculin reinforces cytoskeletal anchorage during cell adhesion. Activating ligands classically disrupt intramolecular interactions between the vinculin head and tail domains that bind to actin filaments. Here, we show that ShigellaIpaA triggers major allosteric changes in the head domain, leading to vinculin homo-oligomerization. Through the cooperative binding of its three vinculin-binding sites (VBSs), IpaA induces a striking reorientation of the D1 and D2 head subdomains associated with vinculin oligomerization. IpaA thus acts as a catalyst producing vinculin clusters that bundle actin at a distance from the activation site and trigger the formation of highly stable adhesions resisting the action of actin relaxing drugs. Unlike canonical activation, vinculin homo-oligomers induced by IpaA appear to keep a persistent imprint of the activated state in addition to their bundling activity, accounting for stable cell adhesion independent of force transduction and relevant to bacterial invasion.

Published
2023
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35. Cytoskeletal mechanics during S higella invasion and dissemination in epithelial cells.

Author

Valencia‐Gallardo, Cesar M., Carayol, Nathalie, and Tran Van Nhieu, Guy

Subjects
*BACTERIA cytoskeleton, *ACTIN, *BIOMECHANICS, *SHIGELLA, *EPITHELIAL cells, *MICROBIAL invasiveness
Abstract

The actin cytoskeleton is key to the barrier function of epithelial cells, by permitting the establishment and maintenance of cell-cell junctions and cell adhesion to the basal matrix. Actin exists under monomeric and polymerized filamentous form and its polymerization following activation of nucleation promoting factors generates pushing forces, required to propel intracellular microorganisms in the host cell cytosol or for the formation of cell extensions that engulf bacteria. Actin filaments can associate with adhesion receptors at the plasma membrane via cytoskeletal linkers. Membrane anchored to actin filaments are then subjected to the retrograde flow that may pull membrane-bound bacteria inside the cell. To induce its internalization by normally non-phagocytic cells, bacteria need to establish adhesive contacts and trick the cell into apply pulling forces, and/or to generate protrusive forces that deform the membrane surrounding its contact site. In this review, we will focus on recent findings on actin cytoskeleton reorganization within epithelial cells during invasion and cell-to-cell spreading by the enteroinvasive pathogen S higella, the causative agent of bacillary dysentery. [ABSTRACT FROM AUTHOR]

Published
2015
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36. ShigellaIpaA Binding to Talin Stimulates Filopodial Capture and Cell Adhesion

Author

Valencia-Gallardo, Cesar, Bou-Nader, Charles, Aguilar-Salvador, Daniel-Isui, Carayol, Nathalie, Quenech’Du, Nicole, Pecqueur, Ludovic, Park, HaJeung, Fontecave, Marc, Izard, Tina, and Tran Van Nhieu, Guy

Abstract

The Shigellatype III effector IpaA contains three binding sites for the focal adhesion protein vinculin (VBSs), which are involved in bacterial invasion of host cells. Here, we report that IpaA VBS3 unexpectedly binds to talin. The 2.5 Å resolution crystal structure of IpaA VBS3 in complex with the talin H1–H4 helices shows a tightly folded α-helical bundle, which is in contrast to the bundle unraveling upon vinculin interaction. High-affinity binding to talin H1–H4 requires a core of hydrophobic residues and electrostatic interactions conserved in talin VBS H46. Remarkably, IpaA VBS3 localizes to filopodial distal adhesions enriched in talin, but not vinculin. In addition, IpaA VBS3 binding to talin was required for filopodial adhesions and efficient capture of Shigella. These results point to the functional diversity of VBSs and support a specific role for talin binding by a subset of VBSs in the formation of filopodial adhesions.

Published
2019
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37. Los Proyectos de Energía Eólica Offshore en el País Vasco: los casos de DemoSATH y GEROA

Author

Gutiérrez Basoa, Iker, Yañez Gallardo, Cesar, Herrero Villalibre, Saioa, Máster Universitario en Dirección de Proyectos, and Proiektu Zuzendaritza Unibertsitate Masterra

Subjects
eólica offshore, energía eólica
Abstract

Análisis de la situación de la energía eólica, poniendo el foco en la energía eólica offshore. Estudio de la situación energética del País Vasco, y desarrollo de proyectos de offshore flotante en su costa. Proyectos DemoSATH y GEROA.

Published
2023

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