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15 results on '"Gloc, Ewa"'

5. Ras-Mediated Deregulation of the Circadian Clock in Cancer

Author

Relógio, Angela, primary, Thomas, Philippe, additional, Medina-Pérez, Paula, additional, Reischl, Silke, additional, Bervoets, Sander, additional, Gloc, Ewa, additional, Riemer, Pamela, additional, Mang-Fatehi, Shila, additional, Maier, Bert, additional, Schäfer, Reinhold, additional, Leser, Ulf, additional, Herzel, Hanspeter, additional, Kramer, Achim, additional, and Sers, Christine, additional

Published
2014
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12. [Estimating the efficiency of DNA isolation methods in semen, blood and saliva stains using the QuantiBlot system].

Author

Prośniak A, Gloc E, Berent J, Babol-Pokora K, Jacewicz R, and Szram S

Subjects
Analysis of Variance, Forensic Medicine methods, Humans, Blood Stains, DNA blood, DNA isolation & purification, DNA Fingerprinting methods, Saliva chemistry, Semen chemistry
Abstract

The aim of this study was to compare and select the optimal method of DNA isolation from blood, semen and saliva stains, as well as to determine appropriate conditions for employing amplification kits for identification of individual persons [brak w polskim tekście]. The materials analyzed in this study consisted of stains of blood, semen and saliva samples stored for a year, and stains of blood stored for a month. Seven various methods of isolation were compared: the Fast DNA kit (Qbiogene), phenol/chloroform extraction, Sherlock (DNA II Gdansk), Dneasy (Qiagen), Wizard Genomic Purification Kit (Promega), Chelex 100 (Biorad) and salting out proteins method. After the isolation, the quantity of DNA was measured with QuantiBlot [brak w polskim tekście]. The highest DNA concentration in bloodstains stored for one year and one month was observed employing the salting out proteins method. The phenol-chloroform extraction method was also found to produce reasonably good results. Isolation from blood and semen with salting-out method appeared to be the most effective. The phenol/chloroform method was dependent on the age and origin of the materials [brak w polskim tekście]. The Sherlock kit was proven to be effective in blood samples stored for one year. DNA concentration values obtained in semen and saliva samples were very low and characterized by a low repeatability.

Published
2006

13. [A case of mutation at locus TH01--sequence analysis].

Author

Jacewicz R, Gloc E, Szram S, and Gałecki P

Subjects
Base Sequence, Female, Gene Deletion, Genetics, Population, Humans, Male, Mutation, Paternity, Sequence Analysis, DNA, Tandem Repeat Sequences
Abstract

In a paternity test with 21 short tandem repeats (STRs) an isolated exclusion for the TH01 locus was observed. The probability of paternity or maternity in this case turned out greater than 99,999%. The analysis of sequence indicates only the deletion character of the observed mutation event without any changes in the conservative sequence.

Published
2005

14. [Oxidative stress parameter analysis in rat brains during long-term ethanol intoxication].

Author

Barzdo M, Gloc E, Jurczyk AP, Meissner E, Jankowska B, Berent J, and Szram S

Subjects
Animals, Blood-Brain Barrier drug effects, Brain Chemistry drug effects, Dose-Response Relationship, Drug, Male, Random Allocation, Rats, Rats, Inbred Lew, Sulfhydryl Compounds metabolism, Thiobarbituric Acid Reactive Substances metabolism, Time Factors, Brain drug effects, Brain metabolism, Ethanol toxicity, Lipid Peroxidation drug effects, Oxidative Stress drug effects
Abstract

The aim of this research was to define the influence of long-term ethanol intoxication on the changes in end products of lipid peroxidation, reacting with thiobarbituric acid (TBA-reactive products) and free SH-groups concentrations in rat brains. The experiment was conducted on male Lewis rats. The experimental groups received a 1-molar ethanol solution and the control group received tap water. The animals were killed after 4, 8 and 12 weeks of intoxication and their brains were collected for further examination, encompassing measurement of the concentration of TBA-reactive products and free SH-groups. A statistically significant increase in the concentration of TBA-reactive products in 4th week of intoxication and decrease in the concentration of free SH-groups in 8th and 12th week of intoxication--compared to the control group--was noticed.

Published
2005

15. [Changes of the concentration of lipid peroxidation products and thiol groups in rat brains with long-term intoxication with methanol].

Author

Barzdo M, Gloc E, Meissner E, Jurczyk AP, Jankowska B, Berent J, and Szram S

Subjects
Animals, Brain Chemistry, Male, Oxidative Stress drug effects, Random Allocation, Rats, Rats, Inbred Lew, Thiobarbiturates chemistry, Time Factors, Brain drug effects, Brain metabolism, Lipid Peroxidation drug effects, Methanol toxicity, Sulfhydryl Compounds metabolism
Abstract

The aim of this paper was to define the influence of long-term methanol intoxication on the changes of end products of lipid peroxidation, reacting with thiobarbituric acid (TBA-reactive products) and free SH-group concentration in rat brains. The experiment was conducted on male Lewis rats. The experimental groups received a 1-molar methanol solution and the control group received tap water. The animals were killed after 4, 8 and 12 weeks of intoxication and their brains were collected for further examination, which encompassed measurement of the concentration of TBA-reactive products and free SH-groups. The revealed changes of TBA-reactive products and free SH-group concentration were not statistically significant.

Published
2005

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