Search

Your search keyword '"Han, Jongyoon"' showing total 923 results
Start Over Author "Han, Jongyoon" Publication Year Range Last 50 years
923 results on '"Han, Jongyoon"'

6. Additional contributions from: Nobel Symposium 162 - Microfluidics

Author

Löfås, Stefan, Herr, Amy E., Qin, Jianhua, Knowles, Tuomas, Kitamori, Takehiko, Lu, Hang, Beebe, David J., Han, Jongyoon, Landers, James, Manz, Andreas, Zengerle, Roland, Weitz, David A., Elf, Johan, and Laurell, Thomas

Subjects
Physics - Fluid Dynamics, Quantitative Biology - Other Quantitative Biology
Abstract

Series of short contributions that are part of Nobel Symposium 162 - Microfluidics arXiv:1712.08369., Comment: Nobel Symposium 162, Stockholm, Sweden, 2017 arXiv:1712.08369v1 Report-no: Nobel162/2017/00

Published
2018

7. Theoretical Limits and Scaling Laws for Electrokinetic Molecular Concentration via Ion Concentration Polarization

Author

Ouyang, Wei, Li, Zirui, Ye, Xinghui, and Han, Jongyoon

Subjects
Physics - Fluid Dynamics, Condensed Matter - Soft Condensed Matter
Abstract

We develop the first theoretical model for the analytical description of ion concentration polarization (ICP)-based electrokinetic molecular concentration, which had not been possible due to the extraordinary complexity of the system. We define the two separate limits for the enrichment factor achievable in a given system and derive the scaling laws for critical parameters, which are validated by numerical simulations and experiments. This work provides clear theoretical explanations on the diverse experimental behaviors previously observed yet unexplainable, while setting solid foundation for the engineering of ICP-based concentrators and other fluid-coupled electrokinetic systems.

Published
2018

10. Accurate Multi-physics Numerical Analysis of Particle Preconcentration Based on Ion Concentration Polarization

Author

Li, Zirui, Liu, Wei, Zhu, Yudan, Lu, Xiaohua, Gu, Yuantong, and Han, Jongyoon

Subjects
Physics - Fluid Dynamics
Abstract

This paper studies mechanism of preconcentration of charged particles in a straight micro-channel embedded with permselective membranes, by numerically solving coupled transport equations of ions, charged particles and solvent fluid without any simplifying assumptions. It is demonstrated that trapping and preconcentration of charged particles are determined by the interplay between drag force from the electroosmotic fluid flow and the electrophoretic force applied trough the electric field. Several insightful characteristics are revealed, including the diverse dynamics of co-ions and counter ions, replacement of co-ions by focused particles, lowered ion concentrations in particle enriched zone, and enhanced electroosmotic pumping effect etc. Conditions for particles that may be concentrated are identified in terms of charges, sizes and electrophoretic mobilities of particles and co-ions. Dependences of enrichment factor on cross-membrane voltage, initial particle concentration and buffer ion concentrations are analyzed and the underlying reasons are elaborated. Finally, post priori a condition for validity of decoupled simulation model is given based on charges carried by focused charge particles and that by buffer co-ions. These results provide important guidance in the design and optimization of nanofluidic preconcentration and other related devices., Comment: 18 pages, 11 firgures

Published
2017
Full Text
View/download PDF

11. iSECRETE: Integrating Microfluidics and DNA Proximity Amplification for Synchronous Single‐Cell Activation and IFN‐γ Secretion Profiling.

Author

Lu, Ri, Ang, Yan Shan, Cheung, Ka‐Wai, Quek, Kai Yun, Sin, Wei‐Xiang, Lee, Elizabeth, Lim, Shir Lynn, Yung, Lin‐Yue Lanry, Birnbaum, Michael E., Han, Jongyoon, Cheow, Lih Feng, and Zeming, Kerwin Kwek

Subjects
CHIMERIC antigen receptors, IMMUNOREGULATION, T cells, IMMUNE response, DETECTION limit
Abstract

Cytokines, crucial in immune modulation, impact disease progression when their secretion is dysregulated. Existing methods for profiling cytokine secretion suffer from time‐consuming and labor‐intensive processes and often fail to capture the dynamic nature of immune responses. Here, iSECRETE, an integrated platform that enables synchronous cell activation, wash‐free, and target‐responsive protein detection for single‐cell IFN‐γ cytokine secretion analysis within 30 min at room temperature is presented. By incorporating a DNA proximity assay (DPA) into a multifunctional microfluidic system, one‐pot homogenous cytokine signal amplification, with a limit of detection of ≈50 secreted molecules per cell is achieved. iSECRETE can robustly handle various sample types that are shown. Two distinct immune activation assay modalities are demonstrated on iSECRETE. Finally, the detection of single‐cell IFN‐γ secretion as an activation hallmark of chimeric antigen receptor T cells within 6 h of exposure to cancer targets is shown. iSECRETE represents the fastest single‐cell sample‐to‐result cytokine secretion assay to date, providing a powerful tool for advancing the understanding of biological phenotypes, functions, and pathways under in vivo‐like conditions. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

12. Label‐Free Impedance Analysis of Induced Pluripotent Stem Cell‐Derived Spinal Cord Progenitor Cells for Rapid Safety and Efficacy Profiling.

Author

He, Linwei, Tan, Jerome, Ng, Shi Yan, Li, King Ho Holden, Han, Jongyoon, Chew, Sing Yian, and Hou, Han Wei

Subjects
INDUCED pluripotent stem cells, NEURAL stem cells, CELL size, PROGENITOR cells, CELL differentiation
Abstract

Regenerative therapies, including the transplantation of spinal cord progenitor cells (SCPCs) derived from induced pluripotent stem cells (iPSCs), are promising treatment strategies for spinal cord injuries. However, the risk of tumorigenicity from residual iPSCs advocates an unmet need for rapid SCPCs safety profiling. Herein, a rapid (≈3000 cells min‐1) electrical‐based microfluidic biophysical cytometer is reported to detect low‐abundance iPSCs from SCPCs at single‐cell resolution. Based on multifrequency impedance measurements (0.3 to 12 MHz), biophysical features including cell size, deformability, membrane, and nucleus dielectric properties are simultaneously quantified as a cell is hydrodynamically stretched at a cross junction under continuous flow. A supervised uniform manifold approximation and projection (UMAP) model is further developed for impedance‐based quantification of undifferentiated iPSCs with high sensitivity (≈1% spiked iPSCs) and shows good correlations with SCPCs differentiation outcomes using two iPSC lines. Cell membrane opacity (day 1) is also identified as a novel early intrinsic predictive biomarker that exhibits a strong correlation with SCPC differentiation efficiency (day 10). Overall, it is envisioned that this label‐free and optic‐free platform technology can be further developed as a versatile cost‐effective process analytical tool to monitor or assess stem cell quality and safety in regenerative medicine. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

13. Comparative Analysis of Serum and Serum-Free Medium Cultured Mesenchymal Stromal Cells for Cartilage Repair.

Author

Kang, Meiqi, Yang, Yanmeng, Zhang, Haifeng, Zhang, Yuan, Wu, Yingnan, Denslin, Vinitha, Othman, Rashidah Binte, Yang, Zheng, and Han, Jongyoon

Subjects
SERUM-free culture media, CARTILAGE cells, STROMAL cells, CHONDROGENESIS, BLOOD serum analysis, CARTILAGE regeneration
Abstract

Mesenchymal stromal cells (MSCs) are promising candidates for cartilage repair therapy due to their self-renewal, chondrogenic, and immunomodulatory capacities. It is widely recognized that a shift from fetal bovine serum (FBS)-containing medium toward a fully chemically defined serum-free (SF) medium would be necessary for clinical applications of MSCs to eliminate issues such as xeno-contamination and batch-to-batch variation. However, there is a notable gap in the literature regarding the evaluation of the chondrogenic ability of SF-expanded MSCs (SF-MSCs). In this study, we compared the in vivo regeneration effect of FBS-MSCs and SF-MSCs in a rat osteochondral defect model and found poor cartilage repair outcomes for SF-MSCs. Consequently, a comparative analysis of FBS-MSCs and SF-MSCs expanded using two SF media, MesenCult™-ACF (ACF), and Custom StemPro™ MSC SFM XenoFree (XF) was conducted in vitro. Our results show that SF-expanded MSCs constitute variations in morphology, surface markers, senescence status, differentiation capacity, and senescence/apoptosis status. Highly proliferative MSCs supported by SF medium do not always correlate to their chondrogenic and cartilage repair ability. Prior determination of the SF medium's ability to support the chondrogenic ability of expanded MSCs is therefore crucial when choosing an SF medium to manufacture MSCs for clinical application in cartilage repair. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

17. Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting

Author

Tan Kwan Zen, Nicholas, Zeming, Kerwin Kwek, Teo, Kim Leng, Loberas, Mavis, Lee, Jialing, Goh, Chin Ren, Yang, Da Hou, Oh, Steve, Hui Hoi Po, James, Cool, Simon M., Hou, Han Wei, Han, Jongyoon, Tan Kwan Zen, Nicholas, Zeming, Kerwin Kwek, Teo, Kim Leng, Loberas, Mavis, Lee, Jialing, Goh, Chin Ren, Yang, Da Hou, Oh, Steve, Hui Hoi Po, James, Cool, Simon M., Hou, Han Wei, and Han, Jongyoon

Abstract

The growing interest in regenerative medicine has opened new avenues for novel cell therapies using stem cells. Bone marrow aspirate (BMA) is an important source of stromal mesenchymal stem cells (MSCs). Conventional MSC harvesting from BMA relies on archaic centrifugation methods, often leading to poor yield due to osmotic stress, high centrifugation force, convoluted workflow, and long experimental time (∼2–3 hours). To address these issues, we have developed a scalable microfluidic technology based on deterministic lateral displacement (DLD) for MSC isolation. This passive, label-free cell sorting method capitalizes on the morphological differences between MSCs and blood cells (platelets and RBCs) for effective separation using an inverted L-shaped pillar array. To improve throughput, we developed a novel multi-chip DLD system that can process 2.5 mL of raw BMA in 20 ± 5 minutes, achieving a 2-fold increase in MSC recovery compared to centrifugation methods. Taken together, we envision that the developed DLD platform will enable fast and efficient isolation of MSCs from BMA for effective downstream cell therapy in clinical settings., National Research Foundation Singapore

Published
2024

42. Scalable Mesenchymal Stem Cells Enrichment from Bone Marrow Aspirate using Deterministic Lateral Displacement (DLD) Microfluidics Sorting

Author

Tan, Nicholas, primary, Kwek, Kerwin Zeming, additional, Teo, Kim Leng, additional, Loberas, Mavis, additional, Lee, Jia Ling, additional, Goh, Chin Ren, additional, Yang, Dahou, additional, Oh, Steve, additional, Hui, James, additional, Cool, Simon, additional, Wei Hou, Han, additional, and Han, Jongyoon, additional

Published
2023
Full Text
View/download PDF

43. Enhancing Electrokinetic Transport on the Enamel Surface of Tooth Using Multiscale‐Pore Membranes

Author

Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology. Department of Biological Engineering, Kwon, Hyukjin J, Ban, Sungyong, Lee, Sang J, de Sousa, Frederico B, Han, Jongyoon, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology. Department of Biological Engineering, Kwon, Hyukjin J, Ban, Sungyong, Lee, Sang J, de Sousa, Frederico B, and Han, Jongyoon

Published
2023

46. Ultra-High Throughput Enrichment of Viable Circulating Tumor Cells

Author

Khoo, B. L., Warkiani, M. E., Guan, Guofeng, Tan, Daniel Shao-Weng, Lim, Alvin S. T., Lim, Wan-Teck, Yap, Yoon Sim, Lee, Soo Chin, Soo, Ross A., Han, Jongyoon, Lim, Chwee Teck, MAGJAREVIC, Ratko, Editor-in-chief, Ładyzynsk, Piotr, Series editor, Ibrahim, Fatimah, Series editor, Lackovic, Igor, Series editor, Rock, Emilio Sacristan, Series editor, and Goh, James, editor

Published
2014
Full Text
View/download PDF

50. Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA

Author

Bhattacharyya, Roby P., Walker, Mark, Boykin, Rich, Son, Sophie S., Liu, Jamin, Hachey, Austin C., Ma, Peijun, Wu, Lidan, Choi, Kyungyong, Cummins, Kaelyn C., Benson, Maura, Skerry, Jennifer, Ryu, Hyunryul, Wong, Sharon Y., Goldberg, Marcia B., Han, Jongyoon, Pierce, Virginia M., Cosimi, Lisa A., Shoresh, Noam, Livny, Jonathan, Beechem, Joseph, and Hung, Deborah T.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results