12 results on '"Hancong Liu"'
Search Results
2. CSP I-plus modified rEndostatin inhibits hepatocellular carcinoma metastasis via down-regulation of VEGFA and integrinβ1
- Author
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Xueqin Chen, Yan Wang, Hancong Liu, Jingjing Zhang, Jie Wang, Xiaobao Jin, and Yan Ma
- Subjects
Hepatocellular carcinoma ,Tumor metastasis ,rEndostatin ,Liver-targeting peptide CSP I-plus ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background In our previous study, N end of the Circumsporozoite protein (CSP I-plus) modified recombinant human Endostatin (rEndostatin, endostar) (rES-CSP) was constructed, which had antiangiogenic capability and bound to hepatocellular carcinoma in vivo and in vitro. In this study, the inhibition of rES-CSP on hepatocellular carcinoma metastasis was verified in vivo and in vitro, and its possible mechanism was explored. Methods Firstly, the impact of rES-CSP on the migration, adhesion of hepatoma cell HCCLM3 was identified by wound healing, transwell, and on metastasis of orthotopic xenograft model was identified in nude mouse. Then the expression of metastasis-associated molecules (MMP2, E-cadherin, integrinβ1) and angiogenesis-related factors (VEGFA) in vitro and in vivo were detected by real-time PCR, western blotting, immunohistochemistry. Results Finally, we found that rES-CSP could inhibit the migration and invasion of HCCLM3, and decrease tumor metastasis and growth in nude mouse orthotopic xenograft models. The tumor inhibiting rates of rES-CSP and Endostar were 42.46 ± 5.39% and 11.1 ± 1.88%. The lung metastasis rates of the control, Endostar and rES-CSP were 71, 50, and 42.8%, respectively. Compared with Endostar, rES-CSP significantly down-regulated the expression of VEGFA and integrinβ1. Heparin, a competitive inhibitor of CSP I-plus, which can be bind to the highly-sulfated heparan sulfate proteoglycans (HSPGs) over-expressed in liver and hepatocellular carcinoma, alleviated the down-regulation of VEGFA and integrinβ1. Conclusions These indicate that rES-CSP may play a role in inhibiting tumor growth and metastasis by down-regulating the angiogenic factor VEGF and the metastasis-related molecules or by interfering with HSPGs-mediated tumor metastasis.
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- 2022
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3. Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana
- Author
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Yan Ma, Minhua Xu, Hancong Liu, Tiantian Yu, Ping Guo, Wenbin Liu, and Xiaobao Jin
- Subjects
Gordonia ,Secondary metabolites ,Antimicrobial activity ,Antiproliferative activity ,Periplaneta americana ,Biotechnology ,TP248.13-248.65 ,Microbiology ,QR1-502 - Abstract
Abstract Gordonia sp. are members of the actinomycete family, their contribution to the environment improvement and environmental protection by their biological degradation ability, but there are few studies on the antimicrobial activity of their secondary metabolites. Our team isolated and purified an actinomycete WA 4-31 from the intestinal tract of Periplaneta americana, firstly identified the strain WA 4-31 by the morphological characteristics and the phylogenetic analyses, and found it was completely homologous to the strain of Gordonia terrae from the Indian desert. Meanwhile, actinomycin D (1), actinomycin X2 (2), mojavensin A (3) and cyclic (leucine-leucne) dipeptide (4) were obtained from the EtOAc extract from the broth of WA 4-31. Compounds 1–4 showed anti-fungus activities against Candida albicans, Aspergillus niger, A. fumigatus and Trichophyton rubrum, also anti-MRSA and inhibited Escherichia coli in different degree. Interestingly, we found when 3 was mixed with 4 with ratio of 1:1, the activity of the mixture on anti-Candida albicans was better than the single. Besides, compounds 1–3 had varying degrees of antiproliferative activities on CNE-2 and HepG-2 cell lines. These indicated that Gordonia rare actinomycete from the intestinal tract of Periplaneta americana possessed a potential as a source of active secondary metabolites.
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- 2021
- Full Text
- View/download PDF
4. Prodigiosin from Serratia Marcescens in Cockroach Inhibits the Proliferation of Hepatocellular Carcinoma Cells through Endoplasmic Reticulum Stress-Induced Apoptosis
- Author
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Jie Wang, Hancong Liu, Liuchong Zhu, Jingyi Wang, Xiongming Luo, Wenbin Liu, and Yan Ma
- Subjects
PG ,endoplasmic reticulum stress ,apoptosis ,hepatocellular carcinoma ,Organic chemistry ,QD241-441 - Abstract
Hepatocellular carcinoma (HCC) is the most common primary liver malignant tumor, and the targeted therapy for HCC is very limited. Our previous study demonstrated that prodigiosin(PG), a secondary metabolite from Serratia marcescens found in the intestinal flora of cockroaches, inhibits the proliferation of HCC and increases the expression of CHOP, a marker protein for endoplasmic reticulum stress (ERS)-mediated apoptosis, in a dose-dependent manner. However, the mechanisms underlying the activity of PG in vivo and in vitro are unclear. This study explored the molecular mechanisms of PG-induced ERS against liver cancer in vitro and in vivo. The apoptosis of hepatocellular carcinoma cells induced by PG through endoplasmic reticulum stress was observed by flow cytometry, colony formation assay, cell viability assay, immunoblot analysis, and TUNEL assay. The localization of PG in cells was observed using laser confocal fluorescence microscopy. Flow cytometry was used to detect the intracellular Ca2+ concentration after PG treatment. We found that PG could promote apoptosis and inhibit the proliferation of HCC. It was localized in the endoplasmic reticulum of HepG2 cells, where it induces the release of Ca2+. PG also upregulated the expression of key unfolded response proteins, including PERK, IRE1α, Bip, and CHOP, and related apoptotic proteins, including caspase3, caspase9, and Bax, but down-regulated the expression of anti-apoptotic protein Bcl-2 in liver cancer. Alleviating ERS reversed the above phenomenon. PG had no obvious negative effects on the functioning of the liver, kidney, and other main organs in nude mice, but the growth of liver cancer cells was inhibited by inducing ERS in vivo. The findings of this study showed that PG promotes apoptosis of HCC by inducing ERS.
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- 2022
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5. Comparison of design methods for negative pressure gradient rotary bodies: A CFD study.
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Pingan Liu, Hancong Liu, Yanxi Yang, Mengjun Wang, and Yangguang Sun
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Medicine ,Science - Abstract
Computational fluid dynamics (CFD) simulation is used to test two body design methods which use negative pressure gradient to suppress laminar flow separation and drag reduction. The steady-state model of the Transition SST model is used to calculate the pressure distribution, wall shear stress, and drag coefficient under zero angle of attack at different velocities. Four bodies designed by two different methods are considered. Our results show the first method is superior to the body of Hansen in drag reduction and the body designed by the first method is more likely to obtain the characteristics of suppressing or eliminating separation, which can effectively improve laminar flow coverage to achieve drag reduction under higher Reynolds number conditions. The results show that the negative pressure gradient method can suppress separation and drag reduction better than the second method. This successful design method is expected to open a promising prospect for its application in the design of small drag, small noise subsonic hydrodynamic hull and underwater weapons.
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- 2020
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- View/download PDF
6. On-line outlier detection and data cleaning.
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Hancong Liu, Sirish L. Shah, and Wei Jiang
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- 2004
- Full Text
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7. Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana
- Author
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Tiantian Yu, Xiaobao Jin, Ping Guo, Wenbin Liu, Hancong Liu, Minhua Xu, and Yan Ma
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0301 basic medicine ,Periplaneta americana ,Gordonia terrae ,food.ingredient ,030106 microbiology ,Biophysics ,Antiproliferative activity ,Trichophyton rubrum ,Antimicrobial activity ,Gordonia ,medicine.disease_cause ,Microbiology ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,food ,medicine ,Candida albicans ,Escherichia coli ,biology ,Chemistry ,Secondary metabolites ,Aspergillus niger ,biology.organism_classification ,Antimicrobial ,QR1-502 ,Corpus albicans ,030104 developmental biology ,Original Article ,TP248.13-248.65 ,Biotechnology - Abstract
Microbial secondary metabolites are one of the main sources of bioactive natural products. It is estimated that around 75% of all antibiotics are derived from secondary metabolites produced by filamentous actinomycete. Gordonia sp. are members of the actinomycete family, their contribution to the environment improvement and environmental protection by their biological degradation ability, but there are few studies on the antimicrobial activity of their secondary metabolites. Our team isolated a Gordonia strain WA 4–31 with anti-Candida albicans activity from the intestinal tract of Periplaneta americana in the early stage. In this study, we firstly identified the strain WA 4–31 by the morphological characteristics and the phylogenetic analyses, and found that it homologous to a strain of Gordonia from the Indian desert by 100%. Then four compounds, Actinomycin D (1), Actinomycin X2 (2), Mojavensin A (3) and cyclic (leucine-leucne) dipeptide (4) were purified from the ethyl acetate extract of the fermented broth of the strain. The compounds 1–4 had activities against Candida albicans, Aspergillus niger, Aspergillus fumigatus and Trichophyton rubrum. They also had activities against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli.coli in different degree. Interestingly, we found that when Mojavensin A was mixed with compound 4 ratio of 1:1, the activity of the mixture on anti-Candida albicans was better than the single. Besides, compounds 1–3 had varying degrees of antiproliferative activity on CNE-2 cells and HepG-2 cells. These indicated that Gordonia rare actinomycete from the intestinal tract of Periplaneta americana possessed a potential as a source of active secondary metabolites.
- Published
- 2021
8. Antimicrobial compounds were isolated from the secondary metabolites of endophytic Gordonia in the intestinal tract of Periplaneta americana
- Author
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Xiaobao Jin, Hancong Liu, Minhua Xu, Tiantian Yu, Ping Guo, Yan Ma, and Wenbin Liu
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food.ingredient ,food ,Gordonia ,Biology ,biology.organism_classification ,Antimicrobial ,Periplaneta ,Microbiology - Abstract
Background: As we all know, bacterial and fungal infections have become one of the threats to human health. Microbial secondary metabolites are one of the main sources of bioactive natural products. It is estimated that around 60% of all foregone antibiotics are derived from secondary metabolites produced by filamentous actinomycete bacteria. Gordonia spp. are members of the actinomycete family, their contribution to the environment improvement and environmental protection by their biological degradation ability, but there are few studies on their antimicrobial activity of their secondary metabolites. Our team isolated a Gordonia strain WA 4-31 with anti-Candida albicans activity from the intestinal tract of Periplaneta americana in the early stage.Results: In this study, we firstly identified the strain WA 4-31 by the morphological characteristics and the phylogenetic analyses, and found that it homologous to a strain of Gordonia from the Indian desert (EU333873) by 100%. Then four compounds, Actinomycin D (1), Actinomycin X2 (2), Mojavensin A (3) and cyclic (leucine-leucne) dipeptide (4) were purified from the EtOH extract of the fermented broth of the strain. The compounds 1-4 had activities against Candida albicans, Aspergillus niger, Aspergillus fumigatus and Trichophyton rubrum. They also had activities against MRSA, S.aureus, K.peneumoniae and E.coli in different degree. The minimum inhibitory concentration of Actinomycin D and Actinomycin X2 on MASA was 0.25 μg/mL. Interestingly, we found that when Mojavensin A was mixed with compound 4 ratio of 1:1, the solution of the compounds was better than the single on anti-Candida albicans. Besides, compounds 1-3 had varying degrees of cytotoxicity on CNE-2 cells and HepG-2 cells.Conclusions: The present study firstly reported the antimicrobial compounds isolated from Gordonia. These indicated that rare actinomycetes from the intestinal tract of Periplaneta americana possessed a potential as a source of active secondary metabolites.
- Published
- 2021
9. Comparison of design methods for negative pressure gradient rotary bodies: A CFD study
- Author
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Hancong Liu, Mengjun Wang, Yanxi Yang, Yangguang Sun, and Pingan Liu
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Velocity ,02 engineering and technology ,01 natural sciences ,010305 fluids & plasmas ,Physics::Fluid Dynamics ,Laminar Flow ,0203 mechanical engineering ,Fluid dynamics ,Shear Stresses ,Multidisciplinary ,Angle of attack ,Turbulence ,Physics ,Reynolds number ,Classical Mechanics ,Mechanics ,Drag ,020303 mechanical engineering & transports ,Aspect Ratio ,Physical Sciences ,symbols ,Mechanical Stress ,Medicine ,Research Article ,Drag coefficient ,Materials science ,Reynolds Number ,Surface Properties ,Science ,Geometry ,Fluid Mechanics ,Continuum Mechanics ,symbols.namesake ,Motion ,0103 physical sciences ,Pressure ,Animals ,Computer Simulation ,Fluid Flow ,Pressure gradient ,Ships ,Laminar flow ,Fluid Dynamics ,Models, Theoretical ,Hydrodynamics ,Stress, Mechanical ,Mathematics - Abstract
Computational fluid dynamics (CFD) simulation is used to test two body design methods which use negative pressure gradient to suppress laminar flow separation and drag reduction. The steady-state model of the Transition SST model is used to calculate the pressure distribution, wall shear stress, and drag coefficient under zero angle of attack at different velocities. Four bodies designed by two different methods are considered. Our results show the first method is superior to the body of Hansen in drag reduction and the body designed by the first method is more likely to obtain the characteristics of suppressing or eliminating separation, which can effectively improve laminar flow coverage to achieve drag reduction under higher Reynolds number conditions. The results show that the negative pressure gradient method can suppress separation and drag reduction better than the second method. This successful design method is expected to open a promising prospect for its application in the design of small drag, small noise subsonic hydrodynamic hull and underwater weapons.
- Published
- 2020
10. An adaptive regression adjusted monitoring and fault isolation scheme
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Sirish L. Shah, Wei Jiang, Hancong Liu, and Arun K. Tangirala
- Subjects
Multivariate statistics ,Multivariate analysis ,Computer science ,Applied Mathematics ,Multivariate normal distribution ,computer.software_genre ,Regression ,Fault detection and isolation ,Analytical Chemistry ,Variable (computer science) ,Dimension (vector space) ,Chart ,Data mining ,Algorithm ,computer - Abstract
A new method is proposed in this work to detect and isolate faults in a system consisting of multivariate normal data. The proposed method reduces the variable dimension via a T2 decomposition and combines multivariate monitoring and diagnosis in a unified approach. An adaptive regression adjusted (ARA) chart is introduced to utilize the diagnosis result in order to improve the monitoring scheme. Once an out-of-control alarm is detected, the fault isolation can be done directly. Copyright � 2007 John Wiley & Sons, Ltd.
- Published
- 2006
11. Improving supply chain management with statistical quality methods
- Author
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Hancong Liu
- Published
- 2014
12. A Nearly Optimal Order Policy to Reduce Bullwhip Effect
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Kwok-Leung Tsui, Hancong Liu, and Fugee Tsung
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Information Systems and Management ,Inventory investment ,Supply chain ,Variance (accounting) ,Management Science and Operations Research ,Microeconomics ,Order (exchange) ,Management of Technology and Innovation ,Engineering process control ,Bullwhip effect ,Industrial relations ,Economics ,Econometrics ,Revenue ,Business and International Management ,Distortion (economics) - Abstract
An important supply chain research problem is the bullwhip effect caused by information distortion and variation amplification along a supply chain, which can lead to tremendous inefficiencies, such as excessive inventory investment and lost revenues. Motivated by engineering process control methods, this paper proposes a class of order-up-to policies and develops a nearly optimal policy to reduce the bullwhip effect. The proposed policy can significantly reduce the order variance while keeping the expected cost nearly optimal. According to our numerical studies, the order variance of the nearly optimal policy can be reduced by more than 50% while the expected cost is only slightly greater than that of the optimal policy derived in Lee et al. [12].
- Published
- 2005
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