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Oral microbial ecosystems are vital in maintaining the health of the oral cavity and the entire body. Oral microbiota is associated with the progression of oral diseases such as dental caries, periodontal diseases, head and neck cancer, and several systemic diseases such as cardiovascular disease, rheumatoid arthritis, adverse pregnancy outcomes, diabetes, lung infection, colorectal cancer, and pancreatic cancer. Buccal mucosa, tongue dorsum, hard palate, saliva, palatine tonsils, throat, keratinized gingiva, supra-gingival plaque, subgingival plaque, dentures, and lips are microbial habitats of the oral cavity.

We investigate two-temperature accretion flows onto strongly magnetized compact stars. Matter is accreted in the form of an accretion disc upto the disc radius ($r_{\rm d}$), where, the magnetic pressure exceeds both the gas and ram pressure and thereafter the matter is channelled along the field lines onto the poles. We solve the equations of motion self-consistently along the field lines, incorporating radiative processes like bremsstrahlung, synchrotron and inverse-Comptonization. For a given set of constants of motion, the equations of motion do not produce unique transonic solution. Following the second law of thermodynamics the solution with the highest entropy is selected and thereby eliminating the degeneracy in solution. We study the properties of these solutions and obtain corresponding spectra as a function of the magnetic field ($B_*$), spin period ($P$) and accretion rate of the star ($\dot{M}$). A primary shock is always formed just near the surface. The enhanced radiative processes in this post-shock region slows down the matter and it finally settles on the surface of the star. This post-shock region contributes to $\gtrsim 99.99\%$ of the total luminosity obtained from the accretion flow. It is still important to study the full accretion flow because secondary shocks may be present for some combination of $B_*$, $P$ and ${\dot{M}}$ in addition to primary shocks. We find that secondary shocks, if present, produce an extended emission at higher energies in the spectra., 18 pages, 17 figures and accepted for publication in MNRAS

The fecal flora consists of trillions of bacteria influencing human health and several host factors. Such population-based fecal flora studies are critical to uplift the health status of ethnic tribes from Arunachal Pradesh. This study aimed to analyze the ethnic tribe's biofilm producing antibiotic resistant bacteria and their phyllogenetic analysis in 15 stool samples collected from Adi tribes of Arunachal Pradesh. Of the analyzed samples, 42.85% were Escherichia, 20% lactic acid bacteria, 20% Salmonella, and 17.14% Enterococcus. Escherichia coli, lactic acid bacteria, and Enterococcus sp. emerged as strong biofilm producers; however, Salmonella declined to exhibit characters for a strong biofilm producer. Tetracycline resistance dominated in all the gut bacterial profiles. The 16SrRNA amplified PCR product was used for sequencing, and a phylogenetic tree was constructed exhibiting the relationship between the isolates. The test sequences were compared with the non-redundant Gene bank collection of the database with the Basic Local Alignment Search Tool.

Antibiotic resistant bacteria have created serious health conditions worldwide, disseminating various infections to people and community along with direct clinical implications in therapeutic options.The present study analysed 20 samples from human faeces of Apatani tribe, Arunachal Pradesh, India. Biofilm assay, antimicrobial susceptibility tests and antimicrobial profiling were performed along with phylogenetic analysis.Phenotypic screening indicated the presence of 21 aerobic isolates comprising Escherichia sp 42.8% (n ​= ​9), Citrobacter sp 9.52% (n ​= ​2), Klebsiella sp 23.8% (n ​= ​5) and Enterococcus sp 23.8% (n ​= ​5). Tetracycline, ciprofloxacin, ceftadizime, gentamicine, vancomycin and erythromycin were observed to highly dominate the biofilm producing bacteria. Antimicrobial activity of Escherichia sp, Citrobacter sp, Klebsiella sp, and Enterococcus sp inhibited the growth of at least one of the tested pathogens. Phylogenetic analysis revealed that antibiotic resistant Klebsiella sp belonged to Klebsiella pneumonia; Escherichia sp belonged to Escherichia fergusonii and Escherichia coli; Enterococcus sp belonged to Enterococcus faecium while Citrobacter sp belonged to Citrobacter freundii.The present work shows that antibiotic resistant bacteria-Klebsiella sp, Enterococcus sp, Escherichia sp and Citrobacter sp were highly prevalent in the faecal microbial communities of Apatani tribe from Arunachal Pradesh. Presence of such antibiotic resistance and biofilm formation in faecal microbiota poses serious concerns regarding health and therapeutic options as this tribe mostly resides in remote vicinities of Arunachal Pradesh. Thus, exploring the mechanisms for dissemination of antibiotic resistance in this tribe helped us to identify key factors pertaining to the health of this tribe as well as their environment.

We study time-dependent relativistic jets under the influence of radiation field of the accretion disk. The accretion disk consists of an inner compact corona and an outer sub-Keplerian disk. The thermodynamics of the fluid is governed by a relativistic equation of state (EoS) for multispecies fluid which enables to study the effect of composition on jet-dynamics. Jets originate from the vicinity of the central black hole where the effect of gravity is significant and traverses large distances where only special relativistic treatment is sufficient. So we have modified the flat metric to include the effect of gravity. In this modified relativistic framework we have developed a new total variation diminishing (TVD) routine along with multispecies EoS for the purpose. We show that the acceleration of jets crucially depends on flow composition. All the results presented are transonic in nature, starting from very low injection velocities, the jets can achieve high Lorentz factors. For sub-Eddington luminosities, lepton dominated jets can be accelerated to Lorentz factors > 50. The change in radiation field due to variation in the accretion disk dynamics will be propagated to the jet in a finite amount of time. Hence any change in radiation field due to a change in disk configuration will affect the lower part of the jet before it affects the outer part. This can drive shock transition in the jet flow. Depending upon the disk oscillation frequency, amplitude and jet parameters these shocks can collide with each other and may trigger shock cascades., 21 pages, 11 figures. Accepted for publication in ApJ

Dysbiosis of the gut microbiome has been associated with the development of colorectal cancer (CRC). Gut microbiota is involved in the metabolic transformations of dietary components into oncometabolites and tumor-suppressive metabolites that in turn affect CRC development. In a healthy colon, the major of microbial metabolism is saccharolytic fermentation pathways. The alpha-bug hypothesis suggested that oncogenic bacteria such as enterotoxigenic Bacteroides fragilis (ETBF) induce the development of CRC through direct interactions with colonic epithelial cells and alterations of microbiota composition at the colorectal site. Escherichia coli, E. faecalis, F. nucleatum, and Streptococcus gallolyticus showed higher abundance whereas Bifidobacterium, Clostridium, Faecalibacterium, and Roseburia showed reduced abundance in CRC patients. The alterations of gut microbiota may be used as potential therapeutic approaches to prevent or treat CRC. Probiotics such as Lactobacillus and Bifidobacterium inhibit the growth of CRC through inhibiting inflammation and angiogenesis and enhancing the function of the intestinal barrier through the secretion of short-chain fatty acids (SCFAs). Crosstalk between lifestyle, host genetics, and gut microbiota is well documented in the prevention and treatment of CRC. Future studies are required to understand the interaction between gut microbiota and host to the influence and prevention of CRC. However, a better understanding of bacterial dysbiosis in the heterogeneity of CRC tumors should also be considered. Metatranscriptomic and metaproteomic studies are considered a powerful omic tool to understand the anti-cancer properties of certain bacterial strains. The clinical benefits of probiotics in the CRC context remain to be determined. Metagenomic approaches along with metabolomics and immunology will open a new avenue for the treatment of CRC shortly. Dietary interventions may be suitable to modulate the growth of beneficial microbiota in the gut.

Advanced combinatorial treatments of surgery, chemotherapy, and radiotherapy do not have any effect on the enhancement of a 5-year survival rate of oral squamous cell carcinoma (OSCC). The discovery of early diagnostic non-invasive biomarkers is required to improve the survival rate of OSCC patients. Recently, it has been reported that oral microbiome has a significant contribution to the development of OSCC. Oral microbiome induces inflammatory response through the production of cytokines and chemokines that enhances tumor cell proliferation and survival. The study aims to develop saliva-based oral microbiome and cytokine biomarker panel that screen OSCC patients based on the level of the microbiome and cytokine differences. We compared the oral microbiome signatures and cytokine level in the saliva of OSCC patients and healthy individuals by 16S rRNA gene sequencing targeting the V3/V4 region using the MiSeq platform and cytokine assay, respectively. The higher abundance of Prevotella melaninogenica, Fusobacterium sp., Veillonella parvula, Porphyromonas endodontalis, Prevotella pallens, Dialister, Streptococcus anginosus, Prevotella nigrescens, Campylobacter ureolyticus, Prevotella nanceiensis, Peptostreptococcus anaerobius and significant elevation of IL-8, IL-6, TNF-α, GM-CSF, and IFN-γ in the saliva of patients having OSCC. Oncobacteria such as S. anginosus, V. parvula, P. endodontalis, and P. anaerobius may contribute to the development of OSCC by increasing inflammation via increased expression of inflammatory cytokines such as IL-6, IL-8, TNF-α, IFN-γ, and GM-CSF. These oncobacteria and cytokines panels could potentially be used as a non-invasive biomarker in clinical practice for more efficient screening and early detection of OSCC patients.

Biofilm formation on biotic or abiotic surfaces is caused by microbial cells of a single or heterogeneous species. Biofilm protects microbes from stressful environmental conditions, toxic action of chemicals, and antimicrobial substances. Quorum sensing (QS) is the generation of autoinducers (AIs) by bacteria in a biofilm to communicate with one other. QS is responsible for the growth of biofilm, synthesis of exopolysaccharides (EPS), and bioremediation of environmental pollutants. EPS is used for wastewater treatment due to its three-dimensional matrix which is composed of proteins, polysaccharides, humic-like substances, and nucleic acids. Autoinducers mediate significantly the degradation of environmental pollutants. Acyl-homoserine lactone (AHL) producing bacteria as well as quorum quenching enzyme or bacteria can effectively improve the performance of wastewater treatment. Biofilms-based reactors due to their economic and ecofriendly nature are used for the treatment of industrial wastewaters. Electrodes coated with electro-active biofilm (EAB) which are obtained from sewage sludge, activated sludge, or industrial and domestic effluents are getting popularity in bioremediation. Microbial fuel cells are involved in wastewater treatment and production of energy from wastewater. Synthetic biological systems such as genome editing by CRISPR-Cas can be used for the advanced bioremediation process through modification of metabolic pathways in quorum sensing within microbial communities. This narrative review discusses the impacts of QS regulatory approaches on biofilm formation, extracellular polymeric substance synthesis, and role of microbial community in bioremediation of pollutants from industrial effluents.

Metastasis consists of hallmark events, including Epithelial-Mesenchymal Transition (EMT), angiogenesis, initiation of inflammatory tumor microenvironment, and malfunctions in apoptosis. Autophagy is known to play a pivotal role in the metastatic process. Autophagy has pulled researchers towards it in recent times because of its dual role in the maintenance of cancer cells. Evidence states that cells undergoing EMT need autophagy in order to survive during migration and dissemination. Additionally, it orchestrates EMT markers in certain cancers. On the other side of the coin, autophagy plays an oncosuppressive role in impeding early metastasis. This review aims to project the interrelationship between autophagy and EMT. Targeting EMT via autophagy as a useful strategy is discussed in this review. Furthermore, for the first time, we have covered the possible reciprocating roles of EMT and autophagy and its consequences in cancer metastasis.

Gut microbiota studies of ethnic populations reveal gut microbial biomarkers for therapeutic options and detection of the disease state. The present study aimed to analyze the gut microbiome signatures in thirty individuals from the Adi, Apatani and Nyshi tribes of Arunachal Pradesh (ten in each cohort) by sequencing the V3 and V4 regions of 16S rRNA on the Illumina MiSeq Platform. The gut microbiome was highly predominated by Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidates in the three studied tribal groups. At the genus level, significant abundance of Bifidobacterium, Collinsella, Bacteroides, Prevotella, Lactobacillus, Streptococcus, Clostridium, Coprococcus, Dorea, Lachnospira, Roseburia, Ruminococcus, Faecalibacterium, Catenibacterium, Eubacterium, Citrobacter and Enterobacter were observed amongst the three tribes. The tribal communities residing in remote areas and following traditional lifestyle had higher gut microbiome diversity with a high prevalence of Prevotella and Collinsella in the Adi and Nyshi tribes, and Bifidobacterium and Catenibacterium in the Apatani tribe. Elucidating the gut microbiome of the tribal community of Arunachal Pradesh will add to the knowledge on relationships between microbial communities, dietary food factors, and the overall state of health of humans worldwide.

The gut bacterial community is involved in the metabolism of bile acids and short-chain fatty acids (SCFAs). Bile acids are involved in the absorption of fat and the regulation of lipid homeostasis through emulsification and are transformed into unconjugated bile acids by the gut microbiota. The gut microbiota is actively involved in the production of bile acid metabolites, such as deoxycholic acid, lithocholic acid, choline, and SCFAs such as acetate, butyrate, and propionate. Metabolites derived from the gut microbiota or modified gut microbiota metabolites contribute significantly to host pathophysiology. Gut bacterial metabolites, such as deoxycholic acid, contribute to the development of hepatocellular carcinoma and colon cancer by factors such as inflammation and oxidative DNA damage. Butyrate, which is derived from gut bacteria such as Megasphaera, Roseburia, Faecalibacterium, and Clostridium, is associated with the activation of Treg cell differentiation in the intestine through histone acetylation. Butyrate averts the action of class I histone deacetylases (HDAC), such as HDAC1 and HDAC3, which are responsible for the transcription of genes such as p21/Cip1, and cyclin D3 through hyperacetylation of histones, which orchestrates G1 cell cycle arrest. It is essential to identify the interaction between the gut microbiota and bile acid and SCFA metabolism to understand their role in gastrointestinal carcinogenesis including colon, gastric, and liver cancer. Metagenomic approaches with bioinformatic analyses are used to identify the bacterial species in the metabolism of bile acids and SCFAs. This review provides an overview of the current knowledge of gut microbiota-derived bile acid metabolism in tumor development and whether it can stand as a marker for carcinogenesis. Additionally, this review assesses the evidence of gut microbiota-derived short-chain fatty acids including butyric acid in antitumor activity. Future research is required to identify the beneficial commensal gut bacteria and their metabolites which will be considered to be therapeutic targets in inflammation-mediated gastrointestinal cancers.

In an attempt to investigate the structures of ultra-relativistic jets injected into the intracluster medium (ICM) and the associated flow dynamics, such as shocks, velocity shear, and turbulence, we have developed a new special relativistic hydrodynamic (RHD) code in the Cartesian coordinates, based on the weighted essentially non-oscillatory (WENO) scheme. It is a finite difference scheme of high spatial accuracy, which has been widely employed for solving hyperbolic systems of conservation equations. The code is equipped with different WENO versions, such as the 5th-order accurate WENO-JS (Jiang & Shu 1996), WENO-Z, and WENO-ZA, and different time integration methods, such as the 4th-order accurate Runge-Kutta (RK4) and strong stability preserving RK (SSPRK), as well as the implementation of the equations of state (EOSs) that closely approximate the EOS of the single-component perfect gas in relativistic regime. In addition, it incorporates a high-order accurate averaging of fluxes along the transverse directions to enhance the accuracy of multi-dimensional problems, and a modification of eigenvalues for the acoustic modes to effectively control the carbuncle instability. Through extensive numerical tests, we assess the accuracy and robustness of the code, and choose WENO-Z, SSPRK, and the EOS suggested in Ryu et al. (2006) as the fiducial setup for simulations of ultra-relativistic jets. The results of our study of ultra-relativistic jets using the code is reported in an accompanying paper (Seo et al. 2021, Paper II)., 21 pages, 10 figures, accepted for publication in ApJ

Non-renewable fossil fuels such as bitumen, coal, natural gas, oil shale, and petroleum are depleting over the world owing to unrestricted consumption. Biofuels such as biodiesel, biobutanol, bioethanol, and biogas are considered an eco-friendly and cost-effective alternatives of fossil fuels. For energy sustainability, the production of advanced biofuels is required. The advancement of genetic and metabolic engineering in microbial cells played a significant contribution to biofuels overproduction. Essential approaches such as next-generation sequencing technologies and CRISPR/Cas9-mediated genome editing of microbial cells are required for the mass manufacture of biofuels globally. Advanced "omics" approaches are used to construct effective microorganisms for biofuels manufacturing. A new investigation is required to augment the production of lignocellulosic-based biofuels with minimal use of energy. Advanced areas of metabolic engineering are introduced in the manufacture of biofuels by the use of engineered microbial strains. Genetically modified microorganisms are used for the production of biofuels in large quantities at a low-cost.

Gut microbiome alterations may play a paramount role in determining the clinical outcome of clinical COVID-19 with underlying comorbid conditions like T2D, cardiovascular disorders, obesity, etc. Research is warranted to manipulate the profile of gut microbiota in COVID-19 by employing combinatorial approaches such as the use of prebiotics, probiotics and symbiotics. Prediction of gut microbiome alterations in SARS-CoV-2 infection may likely permit the development of effective therapeutic strategies. Novel and targeted interventions by manipulating gut microbiota indeed represent a promising therapeutic approach against COVID-19 immunopathogenesis and associated co-morbidities. The impact of SARS-CoV-2 on host innate immune responses associated with gut microbiome profiling is likely to contribute to the development of key strategies for application and has seldom been attempted, especially in the context of symptomatic as well as asymptomatic COVID-19 disease.

We study the evolution of one-dimensional relativistic jets, using the exact solution of the Riemann problem for relativistic flows. For this purpose, we solve equations for the ideal special relativistic fluid composed of dissimilar particles in flat space-time and the thermodynamics of fluid is governed by a relativistic equation of state. We obtain the exact solution of jets impinging on denser ambient media. The time variation of the cross-section of the jet-head is modeled and incorporated. We present the initial condition that gives rise to a reverse shock. If the jet-head cross-section increases in time, the jet propagation speed slows down significantly and the reverse-shock may recede opposite to the propagation direction of the jet. We show that the composition of jet and ambient medium can affect the jet solution significantly. For instance, the propagation speed depends on the composition and is maximum for a pair-dominated jet, rather than a pure electron-positron or electron-proton jet. The propagation direction of the reverse-shock may also strongly depend on the composition of the jet., Accepted for publication in MNRAS; 19 pages, 18 figures

Major contaminants wastewaters are organic compounds, xenobiotics, metals, suspended soils, nutrients (mainly nitrogen and phosphorus), and pathogenic microorganisms. Biological wastewater treatment systems have been used to remove domestic and industrial wastes from water. Wastewater treatment plants (WWTPs) are considered as a barrier and dissemination point of antibiotic-resistant bacteria. Denitrification, ammonification, nitrogen fixation, and nitrification are key parameters of nitrogen cycle in wastewater treatment. Highly complex and diverse microbial communities are predominant in wastewater treatment systems. These microbial communities affect each other in stressful environmental conditions. To understand better interactions between these complex microbial communities, omics approaches such as genomics, transcriptomics, proteomics, and metabolomics are need to apply in WWTPs. The generation of large next-generation sequencing data will provide taxonomic and functional diversity of environmental microbial communities. Metatranscriptomics and metaproteomics approaches identify the functional dynamics of microbial communities in environmental processes. This chapter represents the signatures of microbial communities that are involved in the dissemination of antibiotic resistance genes and nutrient-removing in WWTPs by using metagenomic, metatranscriptomic, and metaproteomic approaches.

Antibiotic resistance is recognized as one of the most important serious public health problem. Together with antibiotics, antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are introduced into wastewater. Wastewater treatment plants (WWTPs) are considered as a source antibiotic resistance dissemination in the environment due to horizontal transfer of ARGs among different microorganisms. In fact, genes conferring resistance to all classes of antibiotics together with mobile genetic elements like plasmids, transposons, bacteriophages, and integrons carrying ARGs are detected in WWTPs in different countries. Metagenomic analyses have shown that genera such as Enterococcus or Escherichia, often targeted in antibiotic resistance culture-dependent studies in WWTPs, are not detected among the most abundant groups. Proteobacteria followed by Firmicutes and Actinobacteria and members of the genera Aeromonas, Bacillus, Lysinibacillus, Microbacterium, Providencia, and Staphylococcus were predominant bacteria observed in both influent and effluent of WWTP samples. Increasing evidence has indicated that sewage treatment plants serve as essential reservoirs for tetracycline-resistant bacteria and resistance genes (tet) to the environment. Proper treatment of wastewater is essential to prevent the spread of ARB and ARGs into the environment prior to its discharge into rivers, lakes, or sewage systems. The accelerating development of antibiotic resistance among bacteria is a challenging problem, requiring improvement in treatment processes to the next decade.

Objective Differential alterations in gut microbiota and chronic low-grade inflammation play a critical role in the development of Type 2 diabetes (T2D). Here we aimed to investigate if dysbiosis of inflammation and anti-inflammation-associated gut bacterial communities in fecal samples of individuals had any influence on T2D using a 16S rRNA gene of V3 region sequencing at Illumina MiSeq platform. Results Our findings showed that a higher abundance of inflammatory bacteria such as Lactobacillus ruminis, Ruminococcus gnavus, Bacteroides caccae, Butyricimonas, and Collinsella aerofaciens, and lower abundance of anti-inflammatory bacteria such as Faecalibacterium prausnitzii, and Butyrivibrio that likely play a role in the development of T2D. Our findings hint the potential of indigenous microbiota in developing diagnostic markers and therapeutic targets in T2D.