1. Silicone membrane equilibrator: measuring chemical activity of nonpolar chemicals with poly(dimethylsiloxane) microtubes immersed directly in tissue and lipids
- Author
-
Mayer, Philipp, Torang, Lars, Glaesner, Nadia, and Jonsson, Jan Ake
- Subjects
Tissues -- Properties ,Lipids -- Properties ,Silicon -- Properties ,Dimethylpolysiloxane -- Properties ,Dimethylpolysiloxane -- Measurement ,Biochemistry -- Research ,Chemistry - Abstract
The chemical activity of organic chemicals directs their diffusion and partitioning and is consequently crucial for their transport, distribution, and toxic effects. A silicone membrane equilibrator is introduced for measuring the chemical activity of nonpolar organic chemicals in lipid-rich samples: (I) A 6 m poly(dimethylsiloxane) (PDMS) microtube (300 [micro]m i.d., 640 [micro]m o.d.) was placed in a sample, and a sample--PDMS equilibrium was reached within 10 min for 12 polycyclic aromatic hydrocarbons (PAHs) acting as model compounds. (II) A plug of 100 [micro]L of methanol was pushed through the tube to equilibrate it with the PDMS and thus the sample. (III) This yielded an undiluted methanol extract that was injected into a high-performance liquid chromatograph (HPLC) with multiband fluorescence detection. Quantification limits expressed as unitless chemical activities ranged from 6 x [10.sup.-9] to 5 x [10.sup.-8], and relative standard deviations were from 6% to 19%. Chemical activities of PAHs in mussels from two polluted sites were measured between [10.sup.-7] and [10.sup.-5], activity coefficients for PAHs in vegetable and fish oils hardly differed between oils. This method can be used for internal exposure measurements, for monitoring product safety/conformity, and process control. The method can also be applied to measure total analyte concentrations in lipid-rich samples and oils.
- Published
- 2009