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The paper focuses on the assessment of indoor comfort and energy consumption of a university building in Cyprus, during winter and summer of 2012 and 2013. The aim was to make a comparative study of the occupants’ behaviour and its effects on the building's energy consumption, along with the indoor thermal and visual comfort between the two seasons. American Society of Heating, Refrigerating and Air conditioning Standards are used through a questionnaire campaign and the thermal comfort of occupants is analysed with the indicators of predicted mean vote and predicted percentage dissatisfied. The answers are analysed using SPSS software. The air temperature, the relative humidity and the lighting levels of the building are monitored using temperature, humidity and lux meter tools. The monthly energy consumption cost is also calculated. The results are analysed and comparative studies of the occupants’ behaviour conclude to various patterns of effects on the thermal and visual comfort of the building, as well as on its energy consumption. [ABSTRACT FROM PUBLISHER]