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Your search keyword '"Kong, Fei‐Fei"' showing total 9 results
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9 results on '"Kong, Fei‐Fei"'

8. FOXM1 regulated by ERK pathway mediates TGF-β1-induced EMT in NSCLC.

Author

Kong FF, Zhu YL, Yuan HH, Wang JY, Zhao M, Gong XD, Liu F, Zhang WY, Wang CR, and Jiang B

Subjects
Biomarkers, Tumor metabolism, Butadienes pharmacology, Carcinoma, Non-Small-Cell Lung pathology, Cell Line, Tumor, Cell Movement genetics, Epithelial-Mesenchymal Transition drug effects, Epithelial-Mesenchymal Transition genetics, Forkhead Box Protein M1, Forkhead Transcription Factors genetics, Gene Expression Regulation, Neoplastic drug effects, Humans, Lung Neoplasms pathology, MAP Kinase Signaling System drug effects, Molecular Targeted Therapy, Neoplasm Invasiveness genetics, Nitriles pharmacology, RNA, Small Interfering genetics, Transforming Growth Factor beta1 metabolism, Transgenes genetics, Wound Healing genetics, Carcinoma, Non-Small-Cell Lung metabolism, Forkhead Transcription Factors metabolism, Lung Neoplasms metabolism
Abstract

FOXM1, a member of the Forkhead transcriptional family, plays an important role in the EMT process, and transforming growth factor-β1 (TGF-β1) has been identified as the most potent factor that can independently induce EMT in various types of cancer cells. Here we examine the important role of FOXM1 in TGF-β1-induced EMT and investigate the mechanism underlying the relationship between TGF-β1 and FOXM1. Lentivirus-mediated transfection was used to stably upregulate the expression of FOXM1, and a small interfering RNA (siRNA) was introduced to silence the expression of FOXM1. Transwell and wound-healing assays were then performed to assess the invasion and motility potential of non-small cell lung cancer (NSCLC) cells. The NSCLC cell lines exhibited EMT characteristics, including an elongated fibroblastoid shape, induced expression of EMT marker proteins, and increased migratory and invasive potential after induction with TGF-β1. The overexpression of FOXM1 enhanced TGF-β1-induced EMT in NSCLC cells. Knockdown of FOXM1 reversed TGF-β1-induced EMT in NSCLC cell lines but had no effect on the phosphorylation level of ERK. Additionally, U0126, an ERK signaling inhibitor, exerted a reversible effect on TGF-β1-induced EMT and inhibited FOXM1 expression. FOXM1 regulated by the ERK pathway can mediate TGF-β1-induced EMT in NSCLC and is a potential target for the treatment of NSCLC.

Published
2014
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9. [Study on the flavonoids constituents of Trachelospermum jasminoides].

Author

Tan XQ, Guo LJ, Chen HS, Wu LS, and Kong FF

Subjects
Apigenin chemistry, Apigenin isolation & purification, Ethanol, Flavonoids chemistry, Hesperidin chemistry, Hesperidin isolation & purification, Magnetic Resonance Spectroscopy, Molecular Structure, Plant Leaves chemistry, Plant Stems chemistry, Apocynaceae chemistry, Flavonoids isolation & purification, Plants, Medicinal chemistry
Abstract

Objective: To study the flavonoids constituents of Trachelospemum jasminoides., Methods: The compounds were separated and purified by column chromatography with silica gel, and identified by IR, MS, NMR and 2D-NMR., Results: Six flavonoids were identified as apigenin (I), apigenin 7-O-beta-glucoside (II), apigenin 7-O-beta-neospheroside (III), luteoloside (IV), narngin (V) 6,8-di-C-glucopyanosylapigenin (VI), respectively., Conclusion: Compounds V and VI are isolated from this plant for the first time.

Published
2010

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