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Your search keyword '"Kritzinger, Rinaldo K"' showing total 7 results
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7 results on '"Kritzinger, Rinaldo K"'

5. Combining physicochemical properties and microbiome data to evaluate the water quality of South African drinking water production plants

Author

10067051 - Mienie, Charlotte Maria Susanna, 12540110 - Bezuidenhout, Cornelius Carlos, 34538356 - Maguvu, Tawanda E., 24168610 - Kritzinger, Rinaldo K., 20318634 - Molale-Tom, Lesego Gertrude, 23535334 - Coertze, Roelof Dirk, Maguvu, Tawanda E., Bezuidenhout, Cornelius C., Kritzinger, Rinaldo, Tsolo, Karabo, Plaatjie, Moitshepi, Molale-Tom, Lesego G., Mienie, Charlotte M., Coertze, Roelof D., 10067051 - Mienie, Charlotte Maria Susanna, 12540110 - Bezuidenhout, Cornelius Carlos, 34538356 - Maguvu, Tawanda E., 24168610 - Kritzinger, Rinaldo K., 20318634 - Molale-Tom, Lesego Gertrude, 23535334 - Coertze, Roelof Dirk, Maguvu, Tawanda E., Bezuidenhout, Cornelius C., Kritzinger, Rinaldo, Tsolo, Karabo, Plaatjie, Moitshepi, Molale-Tom, Lesego G., Mienie, Charlotte M., and Coertze, Roelof D.

Abstract

Anthropogenic activities in catchments used for drinking water production largely contaminates source waters, and this may impact the quality of the final drinking water product. These contaminants may also affect taxonomic and functional profiles of the bacterial communities in the drinking water. Here, we report an integrated insight into the microbiome and water quality of four water treatment plants (NWC, NWE, WCA and NWG) that supply portable water to communities in South Africa. A new scoring system based on combined significant changes of physicochemical parameters and microbial abundance from raw to treated water was used to evaluate the effectiveness of the treatment plants at water purification. Physicochemical parameters which include total soluble solids, turbidity, pH, nitrites and phosphorus among others, were measured in source, treated, and distributed water. There were general statistically significant (P ≤ 0.05) differences between raw and treated water, demonstrating the effectiveness of the purification process. Illumina sequencing of the 16S rRNA gene was used for taxonomic profiling of the microbial communities and this data was used to infer functional attributes of the communities. Structure and composition of the bacterial communities differed significantly (P < 0.05) among the treatment plants, only NWE and NWG showed no significant differences (P > 0.05), this correlated with the predicted functional profile of the microbial communities obtained from Phylogenetic Investigation of Communities by Reconstruction of Observed States (PICRUSt), as well as the likely pollutants of source water. Bacteroidetes, Chlorobi and Fibrobacteres significantly differed (P < 0.05) between raw and distributed water. PICRUSt inferred a number of pathways involved in the degradation of xenobiotics such as Dichlorodiphenyltrichloroethane, atrazine and polycyclic aromatic hydrocarbons. More worryingly, was the presence of pathways involved in beta-lactam resistan

Published
2020

6. Combining physicochemical properties and microbiome data to evaluate the water quality of South African drinking water production plants

Author

Moitshepi Plaatjie, Lesego G. Molale-Tom, Tawanda E. Maguvu, Karabo Tsholo, Roelof Dirk Coertze, Charlotte Mienie, Rinaldo Kritzinger, Cornelius Carlos Bezuidenhout, 10067051 - Mienie, Charlotte Maria Susanna, 12540110 - Bezuidenhout, Cornelius Carlos, 34538356 - Maguvu, Tawanda E., 24168610 - Kritzinger, Rinaldo K., 20318634 - Molale-Tom, Lesego Gertrude, and 23535334 - Coertze, Roelof Dirk

Subjects
Water supply, Biochemistry, Turbidity, South Africa, Materials Physics, Natural Resources, Water Quality, RNA, Ribosomal, 16S, Food science, Water pollution, 0303 health sciences, Multidisciplinary, Physics, Genomics, Pollution, Chemistry, Medical Microbiology, Physical Sciences, Water Resources, Medicine, Engineering and Technology, Water treatment, Metabolic Pathways, Water Microbiology, Research Article, Environmental Engineering, Water Management, Science, Materials Science, Portable water purification, Microbial Genomics, Biology, Microbiology, Water Purification, 03 medical and health sciences, Water Supply, Genetics, Xenobiotic Metabolism, Humans, 030304 developmental biology, Nitrates, Bacteria, 030306 microbiology, business.industry, Drinking Water, Ecology and Environmental Sciences, Water Pollution, Chemical Compounds, Bacteroidetes, Biology and Life Sciences, biology.organism_classification, Water resources, Metabolism, Water quality, Microbiome, business
Abstract

Anthropogenic activities in catchments used for drinking water production largely contaminates source waters, and this may impact the quality of the final drinking water product. These contaminants may also affect taxonomic and functional profiles of the bacterial communities in the drinking water. Here, we report an integrated insight into the microbiome and water quality of four water treatment plants (NWC, NWE, WCA and NWG) that supply portable water to communities in South Africa. A new scoring system based on combined significant changes of physicochemical parameters and microbial abundance from raw to treated water was used to evaluate the effectiveness of the treatment plants at water purification. Physicochemical parameters which include total soluble solids, turbidity, pH, nitrites and phosphorus among others, were measured in source, treated, and distributed water. There were general statistically significant (P ≤ 0.05) differences between raw and treated water, demonstrating the effectiveness of the purification process. Illumina sequencing of the 16S rRNA gene was used for taxonomic profiling of the microbial communities and this data was used to infer functional attributes of the communities. Structure and composition of the bacterial communities differed significantly (P < 0.05) among the treatment plants, only NWE and NWG showed no significant differences (P > 0.05), this correlated with the predicted functional profile of the microbial communities obtained from Phylogenetic Investigation of Communities by Reconstruction of Observed States (PICRUSt), as well as the likely pollutants of source water. Bacteroidetes, Chlorobi and Fibrobacteres significantly differed (P < 0.05) between raw and distributed water. PICRUSt inferred a number of pathways involved in the degradation of xenobiotics such as Dichlorodiphenyltrichloroethane, atrazine and polycyclic aromatic hydrocarbons. More worryingly, was the presence of pathways involved in beta-lactam resistance, potential pathogenic Escherichia coli infection, Vibrio cholerae infection, and Shigellosis. Also present in drinking and treated water were OTUs associated with a number of opportunistic pathogens.

Published
2020

7. Heterotrophic bacteria in drinking water: evaluating antibiotic resistance and the presence of virulence genes.

Author

Molale-Tom LG, Olanrewaju OS, Kritzinger RK, Fri J, and Bezuidenhout CC

Subjects
Humans, Virulence, Bacteria genetics, Anti-Bacterial Agents pharmacology, Drug Resistance, Microbial, Drinking Water
Abstract

Heterotrophic bacteria, impacting those with infections or compromised immunity, pose heightened health risks when resistant to antibiotics. This study investigates heterotrophic plate count bacteria in water from North West-C (NWC) and North West-G (NWG) facilities, revealing prevalent β-hemolysis (NWC 82.5%, NWG 86.7%), enzyme production (98%), and antibiotic resistance, especially in NWC. NWG exhibits variations in hemolysin ( P = 0.013), lipase ( P = 0.009), and DNase activity ( P = 0.006). Antibiotics, including ciprofloxacin, persist throughout treatment, with high resistance to β-lactams and trimethoprim (47%-100%), predominantly in NWC. Multiple antibiotic resistance index indicates that 90% of values exceed 0.20, signifying isolates from high antibiotic usage sources. Whole genome sequencing reveals diverse antibiotic resistance genes in heterotrophic strains, emphasizing their prevalence and health risks in water.IMPORTANCEThis study's findings are a stark reminder of a significant health concern: our water sources harbor antibiotic-resistant heterotrophic bacteria, which can potentially cause illness, especially in individuals with weakened immune systems or underlying infections. Antibiotic resistance among these bacteria is deeply concerning, as it threatens the effectiveness of antibiotics, critical for treating various infections. Moreover, detecting virulence factors in a notable proportion of these bacteria highlights their elevated risk to public health. This research underscores the immediate need for enhanced water treatment processes, rigorous water quality monitoring, and the development of strategies to combat antibiotic resistance in the environment. Safeguarding the safety of our drinking water is imperative to protect public health and mitigate the spread of antibiotic-resistant infections, making these findings a compelling call to action for policymakers and public health authorities alike., Competing Interests: The authors declare no conflict of interest.

Published
2024
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