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Your search keyword '"Lei, Hongtao"' showing total 701 results
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701 results on '"Lei, Hongtao"'

45. A high affinity and selective DNA aptamer for copper ions.

Author

Wang, Jin, Liu, Yibo, Li, Xiangmei, Lei, Hongtao, and Liu, Juewen

Subjects
COPPER ions, APTAMERS, DETECTION limit, DNA, DETECTORS
Abstract

Capture-SELEX was employed for the selection of DNA aptamers for Cu2+. The best aptamer named Cu-1 has a Kd of 14.2 nM as determined using the strand-displacement assay, representing an approximate 3000-fold improvement over a previously reported Cu2+ aptamer. The sensor achieved a limit of detection of 2.1 nM. [ABSTRACT FROM AUTHOR]

Published
2024
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46. Selection of DNA aptamers for detecting metronidazole and ibuprofen: two common additives in soft drinks.

Author

Wang, Jin, Li, Xiangmei, Lei, Hongtao, and Liu, Juewen

Subjects
ISOTHERMAL titration calorimetry, SOFT drinks, ENVIRONMENTAL monitoring, IBUPROFEN, METRONIDAZOLE, APTAMERS
Abstract

To enhance the effects of some functional soft drinks, drugs, especially metronidazole (MNZ) and ibuprofen (IBF), are often illegally added. This poses a serious threat to the health of consumers. Therefore, developing simple and rapid detection methods for these additives is crucial. In this study, DNA aptamers of metronidazole and ibuprofen were selected using the library-immobilized method. The best aptamer for metronidazole, named MNZ-1, has a dissociation constant (Kd) value of 4.9 μM and the aptamer for ibuprofen, named IBF-1, shows a Kd of 9.3 μM, as determined by the thioflavin T (ThT) fluorescence assay. The Kd values measured using isothermal titration calorimetry (ITC) were 17.0 μM and 66.7 μM for these two aptamers, respectively. Selectivity experiments indicate that MNZ-1 demonstrates very weak binding to structurally similar drugs, whereas IBF-1 exhibits binding capability to some structurally similar compounds comparable to ibuprofen, enabling the simultaneous detection of these types of drugs. Neither MNZ-1 nor IBF-1 binds to other common drugs. Using ThT, a label-free fluorescent detection method was developed for metronidazole and ibuprofen in soft drinks, showing limits of detection (LODs) of 0.6 μM and 4.7 μM, respectively. Owing to their small size and well-defined secondary structures, these aptamers are expected to be utilized in analytical applications for food and environmental monitoring. [ABSTRACT FROM AUTHOR]

Published
2024
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47. Two-Stage Optimization of Mobile Energy Storage Sizing, Pre-Positioning, and Re-Allocation for Resilient Networked Microgrids with Dynamic Boundaries.

Author

Lei, Hongtao, Jiang, Bo, Liu, Yajie, Zhu, Cheng, and Zhang, Tao

Subjects
MICROGRIDS, ENERGY storage, DIRECT costing, ALGORITHMS
Abstract

Networked microgrids (NMGs) enhance the resilience of power systems by enabling mutual support among microgrids via dynamic boundaries. While previous research has optimized the locations of mobile energy storage (MES) devices, the critical aspect of MES capacity sizing has been largely neglected, despite its direct impact on costs. This paper introduces a two-stage optimization framework for MES sizing, pre-positioning, and re-allocation within NMGs. In the first stage, the capacity sizing and pre-positioning of MES devices are optimized before a natural disaster. In the second stage, the re-allocation and active power output of MES devices are adjusted post-disaster, with boundary switches operated based on the damage scenarios. The framework restores unserved loads by either forming isolated microgrids using MES or re-establishing connections between microgrids via smart switches. The proposed framework is modeled mathematically and solved using a customized progressive hedging algorithm. Extensive experiments on modified IEEE 33-node and 69-node systems demonstrate the model's effectiveness and applicability in improving system resilience. [ABSTRACT FROM AUTHOR]

Published
2024
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48. UAV Path Planning Based on Variable Neighborhood Search Genetic Algorithm

Author

Zhang, Guo, Wang, Rui, Lei, Hongtao, Zhang, Tao, Li, Wenhua, Song, Yuanming, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Woeginger, Gerhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Tan, Ying, editor, and Shi, Yuhui, editor

Published
2021
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