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Your search keyword '"Longechamp, Lucie"' showing total 10 results
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10 results on '"Longechamp, Lucie"'

1. Exploring the Therapeutic Potential of Ampelopsis grossedentata Leaf Extract as an Anti-Inflammatory and Antioxidant Agent in Human Immune Cells

Author

Chervet, Arthur, primary, Nehme, Rawan, additional, Decombat, Caroline, additional, Longechamp, Lucie, additional, Habanjar, Ola, additional, Rousset, Amandine, additional, Fraisse, Didier, additional, Blavignac, Christelle, additional, Filaire, Edith, additional, Berthon, Jean-Yves, additional, Delort, Laetitia, additional, and Caldefie-Chezet, Florence, additional

Published
2023
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2. Exploring the Therapeutic Potential of Ampelopsis grossedentata Leaf Extract as an Anti-Inflammatory and Antioxidant Agent in Human Immune Cells.

Author

Chervet, Arthur, Nehme, Rawan, Decombat, Caroline, Longechamp, Lucie, Habanjar, Ola, Rousset, Amandine, Fraisse, Didier, Blavignac, Christelle, Filaire, Edith, Berthon, Jean-Yves, Delort, Laetitia, and Caldefie-Chezet, Florence

Subjects
ANTI-inflammatory agents, REACTIVE oxygen species, NLRP3 protein, CELL communication, INFLAMMASOMES
Abstract

Inflammation is a vital protective response to threats, but it can turn harmful if chronic and uncontrolled. Key elements involve pro-inflammatory cells and signaling pathways, including the secretion of pro-inflammatory cytokines, NF-κB, reactive oxygen species (ROS) production, and the activation of the NLRP3 inflammasome. Ampelopsis grossedentata, or vine tea, contains dihydromyricetin (DHM) and myricetin, which are known for their various health benefits, including anti-inflammatory properties. Therefore, the aim of this study is to assess the impact of an extract of A. grossedentata leaves (50 µg/mL) on inflammation factors such as inflammasome, pro-inflammatory pathways, and macrophage polarization, as well as its antioxidant properties, with a view to combating the development of low-grade inflammation. Ampelopsis grossedentata extract (APG) significantly decreased ROS production and the secretion of pro-inflammatory cytokines (IFNγ, IL-12, IL-2, and IL-17a) in human leukocytes. In addition, APG reduced LPS/IFNγ -induced M1-like macrophage polarization, resulting in a significant decrease in the expression of the pro-inflammatory cytokines TNF-α and IL-6, along with a decrease in the percentage of M1 macrophages and an increase in M0 macrophages. Simultaneously, a significant decrease in NF-κB p65 phosphorylation and in the expression of inflammasome genes (NLRP3, IL-1β and Caspase 1) was observed. The results suggest that Ampelopsis grossedentata could be a promising option for managing inflammation-related chronic diseases. Further research is needed to optimize dosage and administration methods. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Aspalathus linearis (Rooibos) Targets Adipocytes and Obesity-Associated Inflammation

Author

Nehme, Rawan, primary, Chervet, Arthur, additional, Decombat, Caroline, additional, Longechamp, Lucie, additional, Rossary, Adrien, additional, Boutin, Rebecca, additional, Rousset, Amandine, additional, Senejoux, François, additional, Vachias, Caroline, additional, Auxenfans, Céline, additional, Fraisse, Didier, additional, Guyon, Jean-Baptiste, additional, Filaire, Edith, additional, Berthon, Jean-Yves, additional, Diab-Assaf, Mona, additional, Delort, Laetitia, additional, and Caldefie-Chezet, Florence, additional

Published
2023
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4. A bicellular fluorescent ductal carcinoma in situ (DCIS)-like tumoroid to study the progression of carcinoma: practical approaches and optimization

Author

Habanjar, Ola, primary, Maurin, Anne-Catherine, additional, Vituret, Cyrielle, additional, Vachias, Caroline, additional, Longechamp, Lucie, additional, Garnier, Cécile, additional, Decombat, Caroline, additional, Bourgne, Céline, additional, Diab-Assaf, Mona, additional, Caldefie-Chezet, Florence, additional, and Delort, Laetitia, additional

Published
2023
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5. Obésité et progression tumorale : Optimisation d’un modèle 3D de tumoroïdes bicellulaires fluorescents mimant le cancer canalaire in situ dans un microenvironnement adipeux et inflammatoir

Author

Habanjar, Ola, Maurin, Anne-Catherine, Vituret, Cyrielle, Longechamp, Lucie, Garnier, Cecile, Decombat, Caroline, Bourgne, Céline, Auxenfans, Celine, Diab-Assaf, Mona, Caldefie-Chezet, Florence, Delort, Laetitia, Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Faculté de Pharmacie, Role of intra-Clonal Heterogeneity and Leukemic environment in ThErapy Resistance of chronic leukemias (CHELTER), Université Clermont Auvergne (UCA), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), and Université Libanaise

Subjects
[SDV.CAN]Life Sciences [q-bio]/Cancer
Abstract

International audience

Published
2022

6. Développement d’un modèle 3D de tumoroïdes bicellulaires fluorescents mimant le cancer canalaire in situ dans un microenvironnement adipeux et inflammatoire

Author

Delort, Laetitia, Ola, Habanjar, Maurin, Anne-Catherine, Vituret, Cyrielle, Longechamp, Lucie, Garnier, Cecile, Nehme, Rawan, Decombat, Caroline, Bourgne, Céline, Auxenfans, Celine, Diab-Assaf, Mona, Caldefie Chezet, Florence, Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Role of intra-Clonal Heterogeneity and Leukemic environment in ThErapy Resistance of chronic leukemias (CHELTER), Université Clermont Auvergne (UCA), Banque de tissus et cellules, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), and Université Libanaise

Subjects
[SDV.CAN]Life Sciences [q-bio]/Cancer
Abstract

International audience

Published
2022

7. Prévention de la transition du cancer mammaire in situ vers un cancer invasif chez les femmes en surpoids/obèses : développement d'un modèle de tumoroïdes bicellulaires fluorescents

Author

Habanjar, Ola, Maurin, Anne-Catherine, Vituret, Cyrielle, Longechamp, Lucie, Garnier, Cecile, Decombat, Caroline, Bourgne, Céline, Auxenfans, Celine, Diab-Assaf, Mona, Caldefie Chezet, Florence, Delort, Laetitia, Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Faculté de Pharmacie, Role of intra-Clonal Heterogeneity and Leukemic environment in ThErapy Resistance of chronic leukemias (CHELTER), Université Clermont Auvergne (UCA), Banque de tissus et cellules, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), and Université Libanaise

Subjects
[SDV.CAN]Life Sciences [q-bio]/Cancer
Abstract

International audience

Published
2022

8. Practical approaches and optimization of a heterogeneous fluorescent tumoroid generation of ductal breast carcinoma in situ to study the impact of the adipose and inflammatory microenvironment

Author

Habanjar, Ola, Maurin, Anne-Catherine, Vituret, Cyrielle, Longechamp, Lucie, Garnier, Cecile, Decombat, Caroline, Bourgne, Céline, Auxenfans, C., Diab-Assaf, Mona, Caldefie Chezet, Florence, Delort, Laetitia, Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Role of intra-Clonal Heterogeneity and Leukemic environment in ThErapy Resistance of chronic leukemias (CHELTER), Université Clermont Auvergne (UCA), Banque de tissus et de cellules, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), and Université Libanaise

Subjects
[SDV.CAN]Life Sciences [q-bio]/Cancer
Abstract

International audience

Published
2022

9. Impact du microenvironnement inflammatoire et de l'obésité dans la transition du cancer canalaire in situ (CCIS) en carcinome invasif (CCI) via le développement d’un modèle 3D de tumoroïdes fluorescents hétérogènes

Author

Habanjar, Ola, Maurin, Anne-Catherine, Vituret, Cyrielle, Longechamp, Lucie, Garnier, Cecile, Decombat, Caroline, Bourgne, Céline, Auxenfans, C., Diab-Assaf, Mona, Caldefie Chezet, Florence, Delort, Laetitia, Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Université Clermont Auvergne (UCA), Role of intra-Clonal Heterogeneity and Leukemic environment in ThErapy Resistance of chronic leukemias (CHELTER), Banque de tissus et de cellules, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), and Université Libanaise

Subjects
[SDV.CAN]Life Sciences [q-bio]/Cancer
Abstract

International audience

Published
2022

10. A bicellular fluorescent ductal carcinoma in situ(DCIS)-like tumoroid to study the progression of carcinoma: practical approaches and optimization

Author

Habanjar, Ola, Maurin, Anne-Catherine, Vituret, Cyrielle, Vachias, Caroline, Longechamp, Lucie, Garnier, Cécile, Decombat, Caroline, Bourgne, Céline, Diab-Assaf, Mona, Caldefie-Chezet, Florence, and Delort, Laetitia

Abstract

Recently, many types of 3D culture systems have been developed to preserve the physicochemical environment and biological characteristics of the original tumors better than the conventional 2D monolayer culture system. There are various types of models belonging to this culture, such as the culture based on non-adherent and/or scaffold-free matrices to form the tumors. Agarose mold has been widely used to facilitate tissue spheroid assembly, as it is essentially non-biodegradable, bio-inert, biocompatible, low-cost, and low-attachment material that can promote cell spheroidization. As no studies have been carried out on the development of a fluorescent bicellular tumoroid mimicking ductal carcinoma in situ(DCIS) using human cell lines, our objective was to detail the practical approaches developed to generate this model, consisting of a continuous layer of myoepithelial cells (MECs) around a previously formed in situbreast tumor. The practical approaches developed to generate a bi-cellular tumoroid mimicking ductal carcinoma in situ(DCIS), consisting of a continuous layer of myoepithelial cells (MECs) around a previously formed in situbreast tumoroid. Firstly, the optimal steps and conditions of spheroids generation using a non-adherent agarose gel were described, in particular, the appropriate medium, seeding density of each cell type and incubation period. Next, a lentiviral transduction approach to achieve stable fluorescent protein expression (integrative system) was used to characterize the different cell lines and to track tumoroid generation through immunofluorescence, the organization of the two cell types was validated, specific merits and drawbacks were compared to lentiviral transduction. Two lentiviral vectors expressing either EGFP (Enhanced Green Fluorescent Protein) or m-Cherry (Red Fluorescent Protein) were used. Various rates of a multiplicity of infection (MOI) and multiple types of antibodies (anti-p63, anti-CK8, anti-Maspin, anti-Calponin) for immunofluorescence analysis were tested to determine the optimal conditions for each cell line. At MOI 40 (GFP) and MOI 5 (m-Cherry), the signals were almost homogeneously distributed in the cells which could then be used to generate the DCIS-like tumoroids. Images of the tumoroids in agarose molds were captured with a confocal microscope Micro Zeiss Cell Observer Spinning Disk or with IncuCyte® to follow the progress of the generation. Measurement of protumoral cytokines such as IL-6, IL8 and leptin confirmed their secretion in the supernatants, indicating that the properties of our cells were not altered. Finally the advantages and disadvantages of each fluorescent approach were discussed. This model could also be used for other solid malignancies to study the complex relationship between different cells such as tumor and myoepithelial cells in various microenvironments (inflammatory, adipose and tumor, obesity, etc.). Although, this new model is well established to monitor drug screening applications and perform pharmacokinetic and pharmacodynamic analyses.

Published
2023
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