1. Amino Acid Residues Controlling Domain Interaction and Interdomain Electron Transfer in Cellobiose Dehydrogenase
- Author
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Motycka, Bettina, Csarman, Florian, Rupp, Melanie, Schnabel, Karoline, Nagy, Gabor, Karnpakdee, Kwankao, Scheiblbrandner, Stefan, Tscheliessnig, Rupert, Oostenbrink, Chris, Hammel, Michal, and Ludwig, Roland
- Subjects
Biochemistry and Cell Biology ,Chemical Sciences ,Biological Sciences ,Electrons ,Amino Acids ,Fungal Proteins ,Electron Transport ,Carbohydrate Dehydrogenases ,Mixed Function Oxygenases ,Polysaccharides ,Cytochromes ,cellobiose dehydrogenase ,electron transfer ,multistate modeling ,molecular dynamic simulation ,small angle X-ray scattering ,Medicinal and Biomolecular Chemistry ,Organic Chemistry ,Biochemistry and cell biology ,Medicinal and biomolecular chemistry - Abstract
The function of cellobiose dehydrogenase (CDH) in biosensors, biofuel cells, and as a physiological redox partner of lytic polysaccharide monooxygenase (LPMO) is based on its role as an electron donor. Before donating electrons to LPMO or electrodes, an interdomain electron transfer from the catalytic FAD-containing dehydrogenase domain to the electron shuttling cytochrome domain of CDH is required. This study investigates the role of two crucial amino acids located at the dehydrogenase domain on domain interaction and interdomain electron transfer by structure-based engineering. The electron transfer kinetics of wild-type Myriococcum thermophilum CDH and its variants M309A, R698S, and M309A/R698S were analyzed by stopped-flow spectrophotometry and structural effects were studied by small-angle X-ray scattering. The data show that R698 is essential to pull the cytochrome domain close to the dehydrogenase domain and orient the heme propionate group towards the FAD, while M309 is an integral part of the electron transfer pathway - its mutation reducing the interdomain electron transfer 10-fold. Structural models and molecular dynamics simulations pinpoint the action of these two residues on the domain interaction and interdomain electron transfer.
- Published
- 2023