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9 results on '"Maryline Roques"'

1. Cultivar Diversity of Grape Skin Polyphenol Composition and Changes in Response to Drought Investigated by LC-MS Based Metabolomics

Author

Lucie Pinasseau, Anna Vallverdú-Queralt, Arnaud Verbaere, Maryline Roques, Emmanuelle Meudec, Loïc Le Cunff, Jean-Pierre Péros, Agnès Ageorges, Nicolas Sommerer, Jean-Claude Boulet, Nancy Terrier, and Véronique Cheynier

Subjects
grape berry, Vitis vinifera, phenolic compounds, UHPLC-QqQ-MS, metabolomics, water deficit, Plant culture, SB1-1110
Abstract

Phenolic compounds represent a large family of plant secondary metabolites, essential for the quality of grape and wine and playing a major role in plant defense against biotic and abiotic stresses. Phenolic composition is genetically driven and greatly affected by environmental factors, including water stress. A major challenge for breeding of grapevine cultivars adapted to climate change and with high potential for wine-making is to dissect the complex plant metabolic response involved in adaptation mechanisms. A targeted metabolomics approach based on ultra high-performance liquid chromatography coupled to triple quadrupole mass spectrometry (UHPLC-QqQ-MS) analysis in the Multiple Reaction Monitoring (MRM) mode has been developed for high throughput profiling of the phenolic composition of grape skins. This method enables rapid, selective, and sensitive quantification of 96 phenolic compounds (anthocyanins, phenolic acids, stilbenoids, flavonols, dihydroflavonols, flavan-3-ol monomers, and oligomers…), and of the constitutive units of proanthocyanidins (i.e., condensed tannins), giving access to detailed polyphenol composition. It was applied on the skins of mature grape berries from a core-collection of 279 Vitis vinifera cultivars grown with or without watering to assess the genetic variation for polyphenol composition and its modulation by irrigation, in two successive vintages (2014–2015). Distribution of berry weights and δ13C values showed that non irrigated vines were subjected to a marked water stress in 2014 and to a very limited one in 2015. Metabolomics analysis of the polyphenol composition and chemometrics analysis of this data demonstrated an influence of water stress on the biosynthesis of different polyphenol classes and cultivar differences in metabolic response to water deficit. Correlation networks gave insight on the relationships between the different polyphenol metabolites and related biosynthetic pathways. They also established patterns of polyphenol response to drought, with different molecular families affected either positively or negatively in the different cultivars, with potential impact on grape and wine quality.

Published
2017
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2. Exopolysaccharide (EPS) synthesis by Oenococcus oeni: from genes to phenotypes.

Author

Maria Dimopoulou, Marlène Vuillemin, Hugo Campbell-Sills, Patrick M Lucas, Patricia Ballestra, Cécile Miot-Sertier, Marion Favier, Joana Coulon, Virginie Moine, Thierry Doco, Maryline Roques, Pascale Williams, Melina Petrel, Etienne Gontier, Claire Moulis, Magali Remaud-Simeon, and Marguerite Dols-Lafargue

Subjects
Medicine, Science
Abstract

Oenococcus oeni is the bacterial species which drives malolactic fermentation in wine. The analysis of 50 genomic sequences of O. oeni (14 already available and 36 newly sequenced ones) provided an inventory of the genes potentially involved in exopolysaccharide (EPS) biosynthesis. The loci identified are: two gene clusters named eps1 and eps2, three isolated glycoside-hydrolase genes named dsrO, dsrV and levO, and three isolated glycosyltransferase genes named gtf, it3, it4. The isolated genes were present or absent depending on the strain and the eps gene clusters composition diverged from one strain to another. The soluble and capsular EPS production capacity of several strains was examined after growth in different culture media and the EPS structure was determined. Genotype to phenotype correlations showed that several EPS biosynthetic pathways were active and complementary in O. oeni. Can be distinguished: (i) a Wzy-dependent synthetic pathway, allowing the production of heteropolysaccharides made of glucose, galactose and rhamnose, mainly in a capsular form, (ii) a glucan synthase pathway (Gtf), involved in β-glucan synthesis in a free and a cell-associated form, giving a ropy phenotype to growth media and (iii) homopolysaccharide synthesis from sucrose (α-glucan or β-fructan) by glycoside-hydrolases of the GH70 and GH68 families. The eps gene distribution on the phylogenetic tree was examined. Fifty out of 50 studied genomes possessed several genes dedicated to EPS metabolism. This suggests that these polymers are important for the adaptation of O. oeni to its specific ecological niche, wine and possibly contribute to the technological performance of malolactic starters.

Published
2014
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3. A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization

Author

Lucie Pinasseau, Arnaud Verbaere, Maryline Roques, Emmanuelle Meudec, Anna Vallverdú-Queralt, Nancy Terrier, Jean-Claude Boulet, Véronique Cheynier, and Nicolas Sommerer

Subjects
grapes, tannins, phloroglucinolysis, quantitative UHPLC-QqQ-MS, method validation, metabolomics, Organic chemistry, QD241-441
Abstract

A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour. This method was applied to a set of 214 grape skin samples from 107 different red and white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The results of triplicate analyses confirmed the robustness of the method, which was thus proven to be suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape response to drought.

Published
2016
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4. A genome-wide association and prediction study in grapevine deciphers the genetic architecture of multiple traits and identifies genes under many new QTLs

Author

Timothée Flutre, Loïc Le Cunff, Agota Fodor, Amandine Launay, Charles Romieu, Gilles Berger, Yves Bertrand, Nancy Terrier, Isabelle Beccavin, Virginie Bouckenooghe, Maryline Roques, Lucie Pinasseau, Arnaud Verbaere, Nicolas Sommerer, Véronique Cheynier, Roberto Bacilieri, Jean-Michel Boursiquot, Thierry Lacombe, Valérie Laucou, Patrice This, Jean-Pierre Péros, and Agnès Doligez

Subjects
Plant Breeding, Phenotype, Quantitative Trait Loci, Genetics, food and beverages, Chromosome Mapping, Molecular Biology, Polymorphism, Single Nucleotide, Genetics (clinical), Genome-Wide Association Study
Abstract

To cope with the challenges facing agriculture, speeding-up breeding programs is a worthy endeavor, especially for perennial species such as grapevine, but requires understanding the genetic architecture of target traits. To go beyond the mapping of quantitative trait loci in bi-parental crosses, we exploited a diversity panel of 279 Vitis vinifera L. cultivars planted in 5 blocks in the vineyard. This panel was phenotyped over several years for 127 traits including yield components, organic acids, aroma precursors, polyphenols, and a water stress indicator. The panel was genotyped for 63k single nucleotide polymorphisms by combining an 18K microarray and genotyping-by-sequencing. The experimental design allowed to reliably assess the genotypic values for most traits. Marker densification via genotyping-by-sequencing markedly increased the proportion of genetic variance explained by single nucleotide polymorphisms, and 2 multi-single nucleotide polymorphism models identified quantitative trait loci not found by a single nucleotide polymorphism-by-single nucleotide polymorphism model. Overall, 489 reliable quantitative trait loci were detected for 41% more response variables than by a single nucleotide polymorphism-by-single nucleotide polymorphism model with microarray-only single nucleotide polymorphisms, many new ones compared with the results from bi-parental crosses. A prediction accuracy higher than 0.42 was obtained for 50% of the response variables. Our overall approach as well as quantitative trait locus and prediction results provide insights into the genetic architecture of target traits. New candidate genes and the application into breeding are discussed.

Published
2022

5. Genome-wide association and prediction study in grapevine deciphers the genetic architecture of multiple traits and identifies genes under many new QTLs

Author

Timothée Flutre, Loïc Le Cunff, Agota Fodor, Amandine Launay, Charles Romieu, Gilles Berger, Yves Bertrand, Nancy Terrier, Isabelle Beccavin, Virginie Bouckenooghe, Maryline Roques, Lucie Pinasseau, Arnaud Verbaere, Nicolas Sommerer, Véronique Cheynier, Roberto Bacilieri, Jean-Michel Boursiquot, Thierry Lacombe, Valérie Laucou, Patrice This, Jean-Pierre Péros, and Agnès Doligez

Subjects
Evolutionary biology, Genotype, Genetic variation, Trait, food and beverages, Single-nucleotide polymorphism, Genome-wide association study, Heritability, Quantitative trait locus, Biology, Genetic architecture
Abstract

To cope with the challenges faced by agriculture, speeding-up breeding programs is a worthy endeavor, especially for perennials such as grapevine, but requires understanding the genetic architecture of target traits. To go beyond the mapping of quantitative trait locus (QTL) in bi-parental crosses, we exploited a diverse panel of 279 Vitis vinifera L. cultivars. This panel planted in five blocks in the vineyard was phenotyped over several years for 127 traits including yield components, organic acids, aroma precursors, polyphenols, and a water stress indicator. The panel was genotyped for 63k single nucleotide polymorphisms (SNPs) by combining an 18K microarray and genotyping-by-sequencing (GBS). The experimental design allowed to reliably assess the genotypic values for most traits. Marker densification via GBS markedly increased the proportion of genetic variance explained by SNPs, and two multi-SNP models identified QTLs not found by a SNP-by-SNP model. Overall, 489 reliable QTLs were detected for 41% more response variables than by a SNP-by-SNP model with microarray-only SNPs, many new ones compared to the results from bi-parental crosses. Prediction accuracy higher than 0.42 was obtained for 50% of the response variables. Our overall approach as well as QTL and prediction results provide insights into the genetic architecture of target traits. New candidate genes and the application in breeding are discussed.

Published
2020
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6. A fast and robust UHPLC-MRM-MS method to characterize and quantify grape skin tannins after chemical depolymerization

Author

Anna Vallverdú-Queralt, Emmanuelle Meudec, Nancy Terrier, Véronique Cheynier, Arnaud Verbaere, Jean-Claude Boulet, Maryline Roques, Lucie Pinasseau, Nicolas Sommerer, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), European Community (FP7-311775), European Project: 270704, Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)

Subjects
0106 biological sciences, proanthocyanidine, Phloroglucinol, grapes, tannins, phloroglucinolysis, quantitative UHPLC-QqQ-MS, method validation, metabolomics, Pharmaceutical Science, 01 natural sciences, Polymerization, Analytical Chemistry, chemistry.chemical_compound, Tandem Mass Spectrometry, Drug Discovery, Tannin, Vitis, Chromatography, High Pressure Liquid, chemistry.chemical_classification, Vegetal Biology, Molecular Structure, food and beverages, oenologie, Proanthocyanidin, Chemistry (miscellaneous), Molecular Medicine, Mass spectrometry, Vineyard, Article, Catalysis, baie de raisin, lcsh:QD241-441, Metabolomics, lcsh:Organic chemistry, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Proanthocyanidins, Physical and Theoretical Chemistry, Chromatography, Depolymerization, fungi, 010401 analytical chemistry, Organic Chemistry, Selected reaction monitoring, High-Throughput Screening Assays, 0104 chemical sciences, flavane, chemistry, peau, analyse hplc uv, Biologie végétale, 010606 plant biology & botany
Abstract

A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour. This method was applied to a set of 214 grape skin samples from 107 different red and white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The results of triplicate analyses confirmed the robustness of the method, which was thus proven to be suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape response to drought.

Published
2016
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7. Screening germplasm for adaptation to drought and temperature

Author

Lucie Pinasseau, Agnes Doligez, Angelique Adiveze, Agnès Ageorges, Dalel Ahmed, Jean Francois Ballester, Brigitte Barthelemy, Gilles Berger, Yves Bertrand, Virginie Bouckenooghe, Jean Claude Boulet, Timothée Flutre, Agota Fodor, Jean-Luc Guiraud, Amandine Launay, Thierry Lacombe, Therese Marlin, Emmanuelle Meudec, Stephane Nicolas, Lollier, A., Charles Romieu, Maryline Roques, Rémi Guerin Schneider, Nicolas Sommerer, Patrice This, Anna Vallverdu Queralt, Arnaud Verbaere, Loic Lecunff, Veronique Cheynier, Nancy Terrier, Jean-Pierre Peros, Silvia Dal Santo, Sara Zenoni, Giovanni Battista Tornielli, Mario Pezzotti, Carvalho, Luis C., Emanuel Gonçalves, Sara Amancio, Antero Martins, Pablo Carbonell-Bejerano, Rafael Torres-Pérez, María Arrizabalaga, María Oyarzun, Carolina Royo, Elisa Baroja, Enrique García-Escudero, Fermín Morales, Ignacio Pascual, José Martinez-Zapater, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), UMT Qualinnov, Institut Français de la Vigne et du Vin (IFV), UMT Génovigne, Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), University of Verona (UNIVR), Instituto Superior de Agronomia, Instituto de Ciencias de la Vid y el Vino (ICVV), Universidad de La Rioja (UR)-Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Universidad de Navarra [Pamplona] (UNAV), and Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

8. Les tanins du raisin : MRM rapide pour explorer la réponse métabolique de résistance à la sécheresse

Author

Nicolas Sommerer, Lucie Pinasseau, Arnaud Verbaere, Emmanuelle Meudec, Veronique Cheynier, Anna Vallverdu Queralt, Nayla Ferreira Lima, Maryline Roques, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, changement climatique, tanin, tanins, polyphénols, mass spectrum analysis, raisin, tannin, résistance à la sécheresse, polyphénol, oenologie, tannins, spectrométrie de masse, sècheresse, grapes
Abstract

Les tanins du raisin : MRM rapide pour explorer la réponse métabolique de résistance à la sécheresse. Congrès français de Spectrométrie de Masse et d'Analyse Protéomique (SMAP 2015)

Published
2015

9. Exopolysaccharide (EPS) Synthesis by Oenococcus oeni: From Genes to Phenotypes

Author

Maryline Roques, Marion Favier, Virginie Moine, Marguerite Dols-Lafargue, Hugo Campbell-Sills, Magali Remaud-Simeon, Thierry Doco, Melina Petrel, Cécile Miot-Sertier, Marlène Vuillemin, Pascale Williams, Joana Coulon, Patricia Ballestra, Claire Moulis, Maria Dimopoulou, Etienne Gontier, Patrick Lucas, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), USC 1366 Oenologie, Institut National de la Recherche Agronomique (INRA), Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Sciences et Technologies - Bordeaux 1, Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), and Université Sciences et Technologies - Bordeaux 1 (UB)

Subjects
Genotype, bactérie lactique, [SDV]Life Sciences [q-bio], lcsh:Medicine, Biochemistry, Microbiology, oenococcus oeni, Homopolysaccharide, chemistry.chemical_compound, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Gene Order, vin, Genetics, Malolactic fermentation, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, lcsh:Science, Gene, Oenococcus, Phylogeny, Oenococcus oeni, 2. Zero hunger, fermentation alcoolique, Multidisciplinary, biology, lcsh:R, Polysaccharides, Bacterial, Glycosyltransferase Gene, Chromosome Mapping, Biology and Life Sciences, Genomics, Chromosomes, Bacterial, biology.organism_classification, Phenotype, Biosynthetic Pathways, oenologie, chemistry, Genes, Bacterial, Genetic Loci, Multigene Family, Galactose, exopolysaccharide, lcsh:Q, expression des gènes, Research Article, Biotechnology
Abstract

International audience; Oenococcus oeni is the bacterial species which drives malolactic fermentation in wine. The analysis of 50 genomic sequences of O. oeni (14 already available and 36 newly sequenced ones) provided an inventory of the genes potentially involved in exopolysaccharide (EPS) biosynthesis. The loci identified are: two gene clusters named eps1 and eps2, three isolated glycoside-hydrolase genes named dsrO, dsrV and levO, and three isolated glycosyltransferase genes named gtf, it3, it4. The isolated genes were present or absent depending on the strain and the eps gene clusters composition diverged from one strain to another. The soluble and capsular EPS production capacity of several strains was examined after growth in different culture media and the EPS structure was determined. Genotype to phenotype correlations showed that several EPS biosynthetic pathways were active and complementary in O. oeni. Can be distinguished: (i) a Wzy -dependent synthetic pathway, allowing the production of heteropolysaccharides made of glucose, galactose and rhamnose, mainly in a capsular form, (ii) a glucan synthase pathway (Gtf), involved in β-glucan synthesis in a free and a cell-associated form, giving a ropy phenotype to growth media and (iii) homopolysaccharide synthesis from sucrose (α-glucan or β-fructan) by glycoside-hydrolases of the GH70 and GH68 families. The eps gene distribution on the phylogenetic tree was examined. Fifty out of 50 studied genomes possessed several genes dedicated to EPS metabolism. This suggests that these polymers are important for the adaptation of O. oeni to its specific ecological niche, wine and possibly contribute to the technological performance of malolactic starters.

Published
2014
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