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3. Isolation and Detection of Murine iNKT Cells in Different Organs

Author

Xucai Zheng, Haopeng Fang, Xiang Li, Bofeng Li, Li Bai, and Mengqing Cong

Subjects
medicine.anatomical_structure, Immune system, medicine, INKT Cells, chemical and pharmacologic phenomena, Spleen, Biology, Phenotype, Cell biology
Abstract

The invariant NKT (iNKT) cells are innate-like lymphocytes that share phenotypic and functional characteristics with NK cells and T cells, playing an important role in both human and mouse physiology and disease and bridging the gap between the innate and adaptive immune responses. The frequency and subtypes of iNKT cells in major immune organs are different, which also determines the regional immune characteristics of iNKT cells. Here, we report a protocol about the isolation of iNKT cells in the thymus, spleen, and liver of C57BL/6, CD1d-/-, and Jα18-/- mice.

Published
2021

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